EMAGE mouse gene expression spatial database

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EMAGE:14296

Fxyd3 FXYD domain-containing ion transport regulator 3 ( MGI:107497)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004106. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004106_01	euxassay_004106_02	euxassay_004106_03	euxassay_004106_04

euxassay_004106_05	euxassay_004106_06	euxassay_004106_07	euxassay_004106_08	euxassay_004106_09

euxassay_004106_10	euxassay_004106_11	euxassay_004106_12	euxassay_004106_13	euxassay_004106_14

euxassay_004106_15	euxassay_004106_16	euxassay_004106_17	euxassay_004106_18	euxassay_004106_19

euxassay_004106_20	euxassay_004106_21	euxassay_004106_22	euxassay_004106_23	euxassay_004106_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14296_wholemount_strong.wlz

14296_wholemount_moderate.wlz

14296_wholemount_weak.wlz

14296_wholemount_possible.wlz

14296_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14296_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 13 14 15 16

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 18 19 20

vibrissa

moderate

regional

moderate expression: see section 03 04 05 18 19 20

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 07 08 09 10 15 16 17

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14

esophagus

moderate

regional

moderate expression: see section 14

pharynx epithelium

moderate

regional

moderate expression: see section 12 13 14 15 16

tongue epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16

stomach

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11

rectum

moderate

regional

moderate expression: see section 13

midgut

moderate

regional

moderate expression: see section 12 13 14 weak expression: see section 15 16 17 18 19

oral epithelium

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

bladder

strong

regional

strong expression: see section 11 12 13 14

genital tubercle of male

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

urethra of male

strong

regional

strong expression: see section 11 12 13

trachea

moderate

regional

moderate expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5587
Entity Detected:	Fxyd3, FXYD domain-containing ion transport regulator 3 ( MGI:107497)
Sequence:	sense strand is shown >T5587 GCTGCTTTCTCCCGGAACCACCTCTCAGCCTGTTGAGCTACTCAGGTCAAGGCTTTGACATGCAAGAGGT TGTTCTGAGCCTGTTGGTCCTTCTAGCAGGCCTGCCTACTTTGGATGCCAATGACCCTGAAAATAAAAAT GATCCTTTCTACTATGATTGGTACAGCCTCCGAGTCGGCGGGCTCATTTGTGCAGGGATTCTCTGTGCCC TGGGCATTATAGTCCTTATGAGTGGCAAATGCAAATGCAAGTTCAGACAGAAACCCAGTCACCGCCCAGG AGAAGGGCCACCTCTCATCACACCAGGCTCAGCTCACAACTGCTGAAGATGGACCAGTTAAAAGAGCACA GGTCCTGGCTCTGAAGGTGGGCTTGAACTCCGAGCTGGCTGTTCTCCTCCCCTCCTGACACTGCCTTCCC CGAGCCTCATCTCACCCCTCGTGGTAGCAGGCTCTTTGTTCAGTTTTTAATATAAAATGATTTCACATCA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6485767 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6485767 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14300 same embryo
	EMAGE:14297 same embryo
	EMAGE:14299 same embryo
	EMAGE:14301 same embryo
	EMAGE:14298 same embryo
	EurExpress:euxassay_004106 same experiment
	MGI:4824949 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source