EMAGE mouse gene expression spatial database

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EMAGE:14297

Clstn3 calsyntenin 3 ( MGI:2178323)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004100. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004100_01	euxassay_004100_02	euxassay_004100_03	euxassay_004100_04

euxassay_004100_05	euxassay_004100_06	euxassay_004100_07	euxassay_004100_08	euxassay_004100_09

euxassay_004100_10	euxassay_004100_11	euxassay_004100_12	euxassay_004100_13	euxassay_004100_14

euxassay_004100_15	euxassay_004100_16	euxassay_004100_17	euxassay_004100_18	euxassay_004100_19

euxassay_004100_20	euxassay_004100_21	euxassay_004100_22	euxassay_004100_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14297_wholemount_strong.wlz

14297_wholemount_moderate.wlz

14297_wholemount_weak.wlz

14297_wholemount_possible.wlz

14297_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14297_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5629
Entity Detected:	Clstn3, calsyntenin 3 ( MGI:2178323)
Sequence:	sense strand is shown >T5629 GGACGCTGGGCTGGAGACTGAAGAGAGAAGCTACTGCAAGTCCCCCATCTCAGCCACCCAGCGGGTCTGA ACACATCTGCTATATGGACTAGAGTGGTCGGCAGGCTCCTTTCCGATCTTGTCCCCCTGCCCTTCCCTCG TAGTCTCTGCATTTCTCCCCCAAGGTGGCATTAGCAGGCTTGAGGCTCCCAGGGGATAGGAACGCCTGGC CTGGCGTGCTTAGCACCGCACCATGACCCTCCTGCTGGTGTCCCTTTTGCTGGCCTCTCTGCTTCAAATC AGCTCTGGCAACAAAGCCAACAAGCACAAGCCATGGATCGAGGCAGAGTACCAGGGCATTGTCATGGAGA ATGACAACACGGTCCTGTTGAACCCACCACTCTTTGCCTTGGATAAAGATGCTCCACTGCGCTATGCAGG TGAGATCTGCGGCTTCCGGCTCCATGGATCTGGGGTACCCTTCGAGGCTGTGATCCTGGACAAGGCAACG GGTGAAGGGCTGATCCGGGCTAAGGAGCCCGTGGACTGTGAGGCCCAGAAGGAACACACTTTTACCATTC AGGCTTATGACTGTGGCGAGGGACCCGATGGCACCAATACCAAGAAGTCTCACAAGGCAACCGTGCATGT TCGGGTCAATGATGTGAATGAGTTTGCCCCGGTCTTTGTGGAGCGTCTGTACCGTGCTGCAGTGACGGAG GGGAAGCTGTATGATCGGATCTTACGTGTGGAAGCCATTGATGGCGACTGCTCCCCGCAGTACAGCCAGA TCTGCTACTATGAGATCCTTACGCCCAACACCCCTTTCCTCATTGACAATGATGGCAACATTGAGAACAC AGAGAAGCTACAGTACAGTGGCGAGAAACTCTACAAGTTCACAGTGACAGCGTATGACTGTGGGAAGAAG CGAGCGGCAGATGATGCTGAGGTGGAGATTCAGGTGAAGCCCACCTGCAAACCCAGCTGGCAAGGCTGGA ACAAAAGGATTGAATATGCCCCAGGAGCCGGGAGCTTGGCTTTGTTCCCTGGCATCCGTCTGGAGACCTG TGATGAGCCACTGTGGAACATTCAGGCCACCATAGAGCTGCAGACCAGCCATGTGGCCAAGGGCTGCGAC CGAGACAACTATTCAGAGAGGGCTCTGCGGAAGCTCTGTGGTGCTGCTACTGGGGAGGTGGACCTGTTGC CCATGCCCGGCCCCAATGCCAACTGGACAGCGGGGCTGTCGGTGCACTACAGCCAGGACAGCAGCCTCAT CTACTGGTTCAATGGCACACAAGCTGTGCAAGTTCCCCTGGGTGGTCCAGCAGGGCTGGGCTCTGGGCCG CAGGATGGCTTCAGCGACCACTTTACCCTGTCCTTCTGGATGAAGCATAGTGTGACTCCCAGCAAGGGGA AGAAGGAGGAGGAGACCATTGTGTGTAACACAGTCCAGAATGAGGACGGCTACTCTCACTACTCACTGAC TGTCCATGGCTGCAGAATTGCCTTTCTCTACTGGCCGTTGCTTGAGAGTGCTCGTCCTGTCAAGTTCCTC TGGAAGCTGGAGCAGGTGTGTGATGATGAATGGCATCACTACGCCCTGAACCTGGAGTTCCCCACGGTCA CACTGTATACTGACGGTATCTCCTTCGACCCGGCTCTCATCCACGACAATGGTCTCATCCACCCACCCAG GAGGGAACCTGCACTCATGATTGGGGCCTGCTGGACTGAAGAGAAGAACAAAGAGAAGAAGGGGGGAGAG AACAGTACAGACACAGCCTCAGGAGACCCCTTGCTGATCCACCACTACTTTCATGGCTACCTAGCTGGTT TCAGCGTGCGCTCAGGTCGCCTGGAGAGCCGTGAGGTCATCGAGTGCCTCTATGCATGTCGGGAGGGGCT GGACTATAGGGATTTCGAGAGCCTGGGCAAAGGCATGAAGGTCCACGTGAACCCCTCGCAGTCCCTGCTC ACCCTGGAGGGGGATGATGTGGAGACCTTCAACCATGCCCTGCAGCATGTGGCTTACATGAACACTCTGC GCTTTGCCACACCCGGCGTCAGGCCCCTGCGCCTCACCACCGCTGTCAAGTGCTTTAGTGAAGAGTCCTG TGTCTCCATCCCTGAAGTGGAGGGCTATGTGGTGGTTCTTCAGCCCGACGCCCCCCAGATCCTTCTGAGT GGCACAGCTCATTTTGCCCGCCCAGCTGTGGACTTTGAGGGACCCGAGGGAGTCCCCTTGTTCCCCGATC TTCAGATCACCTGCTCCATTTCTCACCAGGTGGAGGCCAAAGCAGATGAGAGTTGGCAGGGCACAGTGAC AGACACACGCATGTCAGATGAGATTGTACACAACTTGGACGGCTGTGAGATTTCTCTGGTGGGGGATGAC CTAGACCCTGAACGAGAGAGCCTGCTCTTGGACATGGCTTCCCTGCAGCAGCGAGGCCTGGAACTCACCA ACACATCTGCCTACCTCACCATTGCTGGGGTGGAGACCATCACTGTGTATGAAGAGATCCTGAGGCAGGC TCGTTATCAGCTTCGGCATGGAGCTGCCCTTTATGCCAGGAAATTCCGGCTTTCTTGCTCGGAGATGAAC GGCCGATACTCCAGCAACGAATTCATTGTGGAGGTCAACGTCCTGCATAGCATGAACCGGGTGGCCCATC CCAGTCATGTGCTCAGTTCACAGCAGTTCCTGCACCGGGGTCACCAGCCTCCTCCTGAGATGGCTGGACA CAGCCTGGCCAGCTCCCACCGGAACTCCATGGTCCCCAGTGCTGCGACTCTCATCATTGTGGTATGCGTG GGCTTTCTGGTGCTTATGGTTATCCTCGGCCTCGTTCGGATCCACTCCCTTCATCGCCGCGTCTCAGGAA CTGGCGGACCTTCAGGGGCTTCCACTGACCCGAAGGACCCTGACCTCTTCTGGGATGACTCTGCTCTCAC CATTATTGTCAATCCTATGGAGTCCTACCAGAACCAGCAGACCTGTGTGGCAGGGGTGGCTGGTGGCCAG CAAGAGGAAGAGGACAGCAGTGACTCGGAAGCAGCCGACTCCCCCAGCAGTGATGAGAGACGCATCATTG AGAGCCCCCCACACCGCTATTGAGGCGCCAACCCTGCCAAGAGAGAGAGGCCTGCCCTGGGGAGACAGGC ACTCAGGAAAAAAAAGGGAGAACGGCATTTTGAGAGAAGAGAGACCGAGGGGGGAGAAAGAGCTCTTCAT TTGGAGCAGTCCTTCAATCTCAGAACCCCAGGAGGCCCCACAGGAGTTGCCCTCCTCCTTCAGCCTGCCC CTGCCTTGGGCTGCCCTTCCCCTGGTGTACTCCTGAGGTGTCTGCCCCAGCTGGCTGCCCTGGGAGAGTG GCTGGATGGCTTTGGCTGCCCTGTAGCCTACCTGTGGCAGCATACTGTGCCAGCCATGCACTGTATTAGG GAGGGGGATGTCAACAGCCTTGGTTGAGGTGGGAATGCAATTGATGGGAGCCTGCTGCTGTGTCCTTCTC TACTCTGTTCTGTCCGTGAGGGCTTTCCTTCTGTTGCCCCTCCCCCTCCCGCTTCCCCTAGGATCCCCTC GCCTCTATTTCCATTTCACTCTGGGGATCCCTGCTCCTCCCTCTTCTCAGCCTCCTCCCTCTTTTGTCCT CTTTTCCTCTCCTGCCTGAGCAGTACCAAGGTCTTACTGTCTCTATTCCTTTCTCCATGGCCTGCTTTGC CTGGGTTGTGGGGAAGGAGGAGGAGGGGGACCCTGGGGGTGTATTATATATAGTGTATATTTTTTCAATG TTGTCGTGAGCGCAGCTCCTGTGTTTCTGTGTGCAGCTCACAGCCTTGTATGTGTGCGTGTGCGTGGCAT TGTCATGTCCTGGGATGAGGGTAGGGGGTGGGTGTGGTAAAGGAGGGCCGCACCCTAGGGTTTTCAAATA AAACAACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6493096 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6493096 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14300 same embryo
	EMAGE:14296 same embryo
	EMAGE:14299 same embryo
	EMAGE:14301 same embryo
	EMAGE:14298 same embryo
	EurExpress:euxassay_004100 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source