EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14299

Slc38a3 solute carrier family 38, member 3 ( MGI:1923507)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004113. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004113_01	euxassay_004113_02	euxassay_004113_03	euxassay_004113_04

euxassay_004113_05	euxassay_004113_06	euxassay_004113_07	euxassay_004113_08	euxassay_004113_09

euxassay_004113_10	euxassay_004113_11	euxassay_004113_12	euxassay_004113_13	euxassay_004113_14

euxassay_004113_15	euxassay_004113_16	euxassay_004113_17	euxassay_004113_18	euxassay_004113_19

euxassay_004113_20	euxassay_004113_21	euxassay_004113_22	euxassay_004113_23	euxassay_004113_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14299_wholemount_strong.wlz

14299_wholemount_moderate.wlz

14299_wholemount_weak.wlz

14299_wholemount_possible.wlz

14299_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14299_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5630
Entity Detected:	Slc38a3, solute carrier family 38, member 3 ( MGI:1923507)
Sequence:	sense strand is shown >T5630 GCTCAGCCTGGAGCAGAGCGGATCTCACCCTCTGGAACGGAGTGTGCTGAACGTGCCCACCACGAGGCCA GGTACGGCCGGGGGATATAGAGCCACCAGCACAGGGGCGCTGGGCAGAAGGAGCCCACAATACCTCCCCC AGTGCATAGTCGGCTCTGGGAAGCTCTCCTTGTACCCCGAGACCCGGGCAGGCGTCTCAGCTTTCCGAGA GTCAGAATCTTGCGAGCTCCAGAGCACAGATACCTGACTGTCAGTGTGAGAGCAGTGTTCTGGGGTGGTC TCTGAGCCATGGAGATACCCCGACAGACAGAGATGGTGGAGCTGGTGCCCAATGGCAAACACTTGGAGGG GCTTCTACCAGTGGGCGTGCCTACAACAGACACCCAGAGGACTGAAGACACCCAACACTGTGGAGAGGGC AAGGGCTTCCTTCAGAAGAGTCCCAGCAAGGAGCCACACTTCACCGATTTCGAGGGGAAGACATCATTTG GGATGTCAGTGTTCAATCTCAGCAACGCCATCATGGGCAGTGGAATTCTGGGGCTCGCCTACGCCATGGC CAATACGGGCATCATCCTTTTCCTGTTCCTGCTTACAGCGGTCGCCCTGTTGTCTAGCTATTCCATCCAC CTGCTCCTCAAGTCTTCGGGGATTGTGGGCATCCGTGCCTACGAGCAGTTGGGCTACCGTGCCTTTGGGA CCCCGGGGAAGCTGGCAGCAGCCCTGGCCATTACGCTTCAGAACATTGGAGCCATGTCCAGCTACCTATA CATCATCAAGTCTGAATTGCCTCTTGTCATACAGACCTTCCTGAATCTGGAGAAGCCGGCCTCGGTGTGG TACATGGATGGCAACTACCTTGTGATCCTGGTTTCTGTCACCATCATTCTGCCCCTAGCACTGATGCGAC AGCTCGGCTACCTGGGTTACTCCAGTGGCTTCTCTCTCAGCTGCATGGTGTTCTTCTTGATCGCAGTCAT CTATAAGAAGTTCCAAGTTCCTTGCCCATTGGCACACAACCTGGCCAATGCCACCGGCAACTTCAGCCAC ATGGTGGTGGCAGAGGAGAAGGCACAGCTGCAGGGCGAGCCTGACACTGCTGCTGAGGCCTTCTGTACCC CAAGCTACTTCACCCTCAACTCACAGACAGCATACACCATCCCCATCATGGCTTTCGCCTTCGTCTGCCA CCCTGAGGTGCTGCCCATATATACAGAGCTCAAGGACCCCTCCAAGAGGAAGATGCAGCACATCTCCAAC CTGTCCATTGCTGTCATGTATGTCATGTACTTCCTGGCCGCCCTCTTCGGCTACCTCACCTTCTACGACG GGGTGGAGTCGGAGCTGCTGCACACCTACAGCAAGGTGGACCCGTTTGATGTGCTGATCTTGTGTGTGCG AGTGGCCGTGCTGATAGCGGTCACACTTACAGTTCCGATTGTTCTGTTCCCGGTACGACGTGCTATCCAG CAGATGCTGTTTCAGAACCAGGAGTTCAGCTGGTTGCGGCACGTGCTCATTGCCACTGGCCTGCTTACGT GCATCAATCTGCTGGTTATCTTCGCCCCCAACATCTTGGGCATATTTGGGATCATTGGTGCCACATCTGC TCCGTGCCTCATCTTCATCTTCCCTGCCATCTTCTACTTCCGAATCATGCCCACTGACAAGGAGCCTGCA AGATCCACCCCTAAAATCCTGGCCCTTTGTTTCGCTGCGGTTGGCTTCCTGCTGATGACCATGAGCCTGA GTTTCATCATCATTGACTGGGTCTCTGGGACCAGCCAGCATGGAGGAAACCATTAGGATGACCTCCATCT TGTCCTGTCTATTCATCCTAGCACCTCCTGCCCTCTCTCTCTCCAGCCCCTGCTCCCAGCCAGGGGCTAA GTAAGGGGAGAGAGACCGGGGAGAAAGACTCTGTAAATACTATGGCCTCTGGCTCCACCCATATCCTCTC CGTTGGAAGGTTTTTGTTGAAGAGCCAGGACCAAGGCCCATGGACCACCCCTGCTGGGCTCCAGAGCTGC AGGGGCTTCCTGCACTAGGAACAGGGTGGGGCTGTCATCGCCTTAGATCCCATGAAACCCTCCATCCTCC TCCAGCAGCTGTGCACACTGAGGCCTAGCGGCCACCTCCTGAGGTCACACAGGCAGTAGGGGCACACAGA GCCAGAAAACCCAGGCCTGAAGACATCCCCTAGTCCTGCTGAGAGACCACAGTCTAAACCCAAGGGCTCA TCCCTGATCTCCAGCCCCGTTTCCAACACTTGGGCCCCAAATCTTGGGTACTCTTGTCTTCTTCCCTCTC CTCCATAGGGGACCCTATCCCATCCCATATTTGGGGTCAGCAGGTTCTGCTGAGGATGGGGCTGGTATGC ACCCCAAAAGATCCCTGCTGTGGGCCTTCACAGTTCAGGGGAGGCTGGGACTCCCACACCTCACGCCTGG GCCATGACTTACATTCCACTGCTGGGAGAAGGGAGGCCAGGCCCAGAGTAGCCTGCCCTTGGGAGTCAAA GACCCTAGAAGCCAGGCTGGCACAGGAATGGGGAGGCTTGCCTTTTATAAATATTATACACAAGAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6493334 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6493334 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14300 same embryo
	EMAGE:14297 same embryo
	EMAGE:14296 same embryo
	EMAGE:14301 same embryo
	EMAGE:14298 same embryo
	EurExpress:euxassay_004113 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source