EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14352

Crym crystallin, mu ( MGI:102675)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004117. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004117_01	euxassay_004117_02	euxassay_004117_03	euxassay_004117_04

euxassay_004117_05	euxassay_004117_06	euxassay_004117_07	euxassay_004117_08	euxassay_004117_09

euxassay_004117_10	euxassay_004117_11	euxassay_004117_12	euxassay_004117_13	euxassay_004117_14

euxassay_004117_15	euxassay_004117_16	euxassay_004117_17	euxassay_004117_18	euxassay_004117_19

euxassay_004117_20	euxassay_004117_21	euxassay_004117_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14352_wholemount_strong.wlz

14352_wholemount_moderate.wlz

14352_wholemount_weak.wlz

14352_wholemount_possible.wlz

14352_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14352_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall marginal layer

strong

regional

strong expression: see section 14 15 16

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 13 14 15

cerebral cortex marginal layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 22

olfactory cortex marginal layer

strong

regional

strong expression: see section 13 14 17 18

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 10

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 13

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 10

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 13

rest of cerebellum mantle layer

strong

regional

strong expression: see section 08 09

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15 16

pons mantle layer

strong

regional

strong expression: see section 06 07 17 18

pons ventricular layer

strong

regional

strong expression: see section 09 15 16

midbrain mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 17 18

midbrain ventricular layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

spinal cord ventricular layer

strong

regional

strong expression: see section 10 11

saccule mesenchyme

strong

regional

strong expression: see section 07 08 09 16 17 18 19

cochlea

strong

regional

strong expression: see section 06 07 08 09 16 17

utricle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 17 18 19 20

utricle mesenchyme

strong

regional

strong expression: see section 04 05 06 07 17 18 19 20

perioptic mesenchyme

strong

regional

strong expression: see section 05 06 07 08 09 22

nasal cavity

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

vomeronasal organ mesenchyme

strong

regional

strong expression: see section 12 13 16 17

lower jaw incisor

strong

regional

strong expression: see section 11 14

lower jaw molar

strong

regional

strong expression: see section 19 20

upper jaw incisor

strong

regional

strong expression: see section 12 13 15 16

upper jaw molar

strong

regional

strong expression: see section 19 20

renal medullary interstitium

strong

regional

strong expression: see section 09 10 11 12 13 18 19 20

genital tubercle of male

strong

regional

strong expression: see section 13 14 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5623
Entity Detected:	Crym, crystallin, mu ( MGI:102675)
Sequence:	sense strand is shown >T5623 CCCGGGTTCGAAGGCAGGCGGCGAGATGAAGCGGGCGCCAGCGTTCCTGAGCGCAGAGGAGGTGCAGGAT CACCTTCGCAGCTCCAGCCTTCTCATCCCACCCCTGGAGGCCGCACTGGCCAACTTCTCCAAAGGTCCCG ACGGAGGGGTCATGCAGCCAGTGCGCACCGTGGTGCCTGTAGCCAAGCACCGAGGCTTCCTGGGAGTCAT GCCTGCCTACAGTGCTGCTGAGGATGCGCTCACCACCAAGTTAGTCACCTTCTATGAGGGCCACAGCAAC ACAGCGGTCCCCTCCCATCAGGCATCGGTGCTTCTCTTTGATCCCAGCAATGGCTCCCTGCTGGCGGTCA TGGATGGAAATGTCATAACTGCAAAGAGAACAGCAGCGGTGTCTGCCATTGCCACAAAGCTGTTGAAGCC CCCAGGCAGTGATGTGCTGTGCATCCTTGGAGCGGGGGTCCAGGCGTACAGTCACTATGAGATCTTCACA GAGCAGTTCTCCTTCAAGGAGGTGAGAATGTGGAACCGCACCAGGGAAAATGCTGAGAAGTTTGCAAGCA CAGTGCAAGGAGATGTTCGGGTCTGTTCATCAGTGCAGGAGGCTGTGACAGGTGCTGATGTCATCATCAC AGTCACCATGGCAACAGAGCCCATTTTATTTGGTGAATGGGTAAAGCCGGGGGCTCACATCAATGCTGTT GGAGCCAGCAGGCCTGACTGGCGAGAACTGGATGACGAGCTCATGAGGCAAGCGGTGCTGTATGTGGACT CCCGGGAGGCTGCCCTGAAGGAGTCAGGAGACGTTCTGTTGTCAGGGGCTGACATCTTTGCTGAGCTTGG AGAAGTGATTTCAGGAGCGAAGCCTGCACACTGTGAGAAGACCACAGTGTTCAAATCTTTGGGGATGGCA GTGGAAGACCTGGTTGCAGCCAAATTAGTATATGATTCTTGGTCATCTGGCAAGTGAGTTGAAGGAACCG TGCCTGAGTTGGCCATCACAGCTCAACACTGTTTCACAAGTGTCAAAATCAAAGGAGGTCCAGTCCCCAG TGAATGGTAGTGATTGTCATTCATAAGTACTGACACCCCTATTCATGTTTGTGGTTGGATAGCTAAACCA GGTAACCATTTCTTCTGTTAAGGGGTGATGGCCACATTATCTACCCTTGATCTTACTAGTCTTGTATCTC TCTGAAATAAATCATTTCCACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6492374 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6492374 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14356 same embryo
	EMAGE:14351 same embryo
	EMAGE:14353 same embryo
	EMAGE:14355 same embryo
	EMAGE:14354 same embryo
	EurExpress:euxassay_004117 same experiment
	MGI:4824074 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source