EMAGE mouse gene expression spatial database

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EMAGE:14353

Chst3 carbohydrate (chondroitin 6/keratan) sulfotransferase 3 ( MGI:1858224)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004138_01

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14353_wholemount_possible.wlz

14353_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14353_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5626
Entity Detected:	Chst3, carbohydrate (chondroitin 6/keratan) sulfotransferase 3 ( MGI:1858224)
Sequence:	sense strand is shown >T5626 CTTAGCCACTGAACCATCTCTCCAGCCTCTGTTTTGGGGTGTTTGTTTGAGACAGAATCAAAAGGCTGGT CTAAAGTACATATTGTAGCTAAAGATGGCTTCCAACTCCTGCCTTGCCTCCAGAGTGTTAGGGTGTGACC TTTAAATGACCTATTAAAGAAACCCCAACAGGTGTAGAGTACACCTGTGATCCCAGCTCTTAGGAGGTTG GACAGATGGATAAAGAGGTCCAGGTGTCCCGGACTACATAGAGAGACCCTATCTCAAAAGAGAGGCGAAG ACTTAGGTTTTGAGAGTGGGCCTGAGGTCTCTCTTCAAAAGTTTTATAGAAAGATGTCTCTGTTCCCTGT CTATGAACACGGATGGCCTGAGCACCTGTCTCTTCACAGGATCAGAGTGTCCCCCACCTGAAGAGGGCTG ATTGGGTCCCAAGCTATGCTCCTGAGCTGAGTGCCTGCAGCCAGTCTGAGGAACTCCATGGCGCCCCCTC TCCCCATGGAGAAAGGACTCGCTTTGCCTCAGGATTTCCGGGACCTTGTACACAGCCTAAAGATTCGAGG CAGATACGTCTTGTTCCTGGCATTTGTGGTCATAGTTTTTATCTTCATTGAAAAGGAAAATAAAATCATA TCCAGGGTCTCCGACAAGCTGAAGCAGATCCCTCATTTTGTGGCAGATGCCAACAGCACTGACCCAGCCC TGCTCTTATCGGAGAATGCATCTCTCTTGTCCCTGAGCGAGTTGGATTCCACCTTTTCCCATCTGCGGAG CCGCCTGCACAACCTGAGCCTGCAGCTGGGCGTGGAGCCAGCAATGGAGAGCCAGGAGGCTGGGGCAGAG AAGCCATCCCAGCAGGCTGGAGCAGGGACCCGGCGCCACGTGCTTCTCATGGCCACCACCCGCACGGGTT CCTCGTTCGTGGGCGAGTTCTTCAACCAGCAGGGCAATATCTTCTACCTCTTCGAGCCACTGTGGCACAT CGAGCGCACCGTGTTCTTCCAGCAGCGAGGCGCCAGCGCGGCTGGTTCAGCCTTGGTCTACCGTGATGTC CTCAAGCAGTTGTTGCTATGCGACCTGTATGTGCTGGAGCCCTTCATCAGCCCTCCGCCCGAGGACCACT TGACTCAGTTCCTGTTCCGCCGGGGATCCAGCCGTTCACTCTGCGAGGATCCGGTGTGCACACCCTTCGT CAAGAAGGTCTTTGAGAAGTACCACTGCAGGAACCGTCGCTGCGGGCCACTCAACGTGACCTTGGCGGGC GAGGCCTGCCGCCGCAAGGACCACGTGGCCCTCAAGGCTGTGCGCATCCGTCAGCTGGAGTTCCTGCAGC CGCTAGTTGAGGACCCGAGGTTGGATCTACGAGTCATTCAGCTGGTGCGCGACCCCCGGGCCGTGCTGGC TTCACGCATAGTGGCCTTTGCGGGCAAGTATGAGAACTGGAAGAAGTGGCTGTCCGAGGGGCAGGACCAG CTGAGCGAGGATGAGGTGCAGCGATTGCGGGGCAACTGTGAGAGCATCCGCCTGTCTGCAGAGCTGGGCT TGCGGCAGCCAGCCTGGCTGCGCGGTCGTTACATGCTGGTGCGCTATGAGGATGTGGCACGCAGGCCACT GCAGAAGGCCCGAGAGATGTACAGCTTTGCGGGCATCCCCTTGACCCCGCAGGTGGAGGACTGGATCCAG AAGAACACGCAGGCGACACGCGACAGCAGCGATGTCTACTCCACTCAGAAAAACTCTTCTGAGCAGTTTG AGAAGTGGCGCTTCAGCATGCCTTTCAAGCTGGCACAGGTGGTACAGGCTGCCTGTGGCCCGACCATGCA CCTCTTTGGCTACAAGTTGGCCAGGGATGCCGCCTCACTCACCAACCGCTCCATCAGCCTGCTGGAGGAG CGGGGCACCTTCTGGGTCACGTAGTGGGGGATGTCTGGGACCCTTGGAACTCCTTCTTGTGAAAGGCTGG CCCTGCTTCCCTCACACCCAGCCTGGCAGTGAGACATAGCCCTGGCAGAAAGTCAAAATGGGGAGCGATG ATGGGAACATAGCCCCTGGCTGTAGCGGTAGGGCCCCCTGCCAGCTAGACTCCCCAGAGCAGCTACAGCT AGGGCCTTGGGCTCCTCTGAGGACACCCTGTCTCCTTAGTGGTACTGGTCATAAGGTGTCCTCACCCTAT GACCTGCAGTGTCCCGAGCAGGTCAAGGTAGGTTCCTGTGTGTGGACACACCTCCCTAGCTCTTTCTCAC ACAGTCTACACGAAGCCTGTAAAGGCCTGTAGGTTGTGTGGGCTGGAGTCCAAGTATTTAACAACCAGAA GGGGTGTAGACCCTCTGCAGCCTCTCAGACCTCCTACTGTATTAAGTGTTAACCTCTTCCGCTCTGAGTC AGAGAAGCTGGGATCTGGCTGAGTCCTGGGAAGGAGGAGGACAGCCTAGGGTGAGGAAGGGGACCTTTGA AGCTCCTCAGGGAACAGTGGCTGTGTACCAGCTCATAGAAAATGGTTTGATCAGCTGTTCTAGTCACTCA TGAGTATCAATCAGCCTGTGTAGAGCAAGACACACAAAGTGCATTGAAAACAGACAAGGCCAGGGGTGTG GCTCAGCGGTAAAGCCCTTGCCTCTCTCATGCCCAGGGCCCTAGGTGTGATTCTTAGCACTATAAATATA AGGGAAAATAAGCCATACATACGTGTGCAGTACAGAGAGAGACAGAAAAGCGTAATAAACATTCCCCCCT CTCATAAGCACACGCTCTGCCTCTGTCTCTGTCTCTGTCTCTCTCTGTCTCTGTCTCTCTCTGTCTCTCT CTCTCACTCTCTCTCTCTCTCTCTCTCTCTCACACACACACACACACACACACACACAAACACACACACA CACACTGGTACAGAAAAGCTTCCCTTCTCCACTCTTTATGCTCACCATGTTTTTAGGATTTTTGTTTGTT TGTTTTGTTTTGGGTTTCTATATATCTAAACAAGGAGCCTTAAATTACATCTTTTTGGGATTACGTGGCG AGGGGGAGCAGCAGGGAGCATTTGCTTCTGGTAAAAGGATAGACCAGAAAGGACTTCCCCTTGGATGATC GGAGTCATGCAGAGCCCCCAGGGTCCCACATGTTCTGTGTGAACTTCATGTGGAATGACTCACAGAAGTG ACTACCTTAGGCGCTCGCTCTGTAGCTCAATGTGCGTAGAGTTTGTCTATTGTATAGGAAGCCCTGGGTT CTGTGCCCTGCCCCACACCATATAGGTATGATGGCGTGTACCCATATCCCAGCATTCAGGAAATCAAAGG TTGGCGGTCAAAAGTTCAAGGTGCAGCTATGTATCTTTTGAGATGTCCGAGTCTATAAGCTCCCTCTACT GAGAGCTGCTAGTATTCCTTCTCAAGAACAGGTTATTTGAAAATCTCTTCTTGTAAGCCCCAACATTTGG GGGAGGGGAGGATGGGGGAACTCTTGACTGAGCAGCTAGGTTTCCCCAAGCAGAAGTTCTTCTCTCCCAG TGCAAGCTGAGGTTTAGTGCCCCGGCTAGCCTCGGAACTGGCTCAGTGGGGGTTGGCACGCTGACTTCCT GGACAATTGCTGCATCTGACACTGTGAGCGCCCAGGACACCGGATGTGTAGCATGGATGTGGGCCTAGCA CAGGTGTGGAAGAGGACAGTCAGGACACTCACTGTTGGTGTTTGTTATCCACCTGTTCAGCCGCCGGGTC CCACACATAGGTCATCTTTCGTCACTCAAGCCGGCCTCTGTTTTTGATTTTAACAATCCAAGAGCAAGTG TGTAGGGGACAAAGAGACACCAGCTCTTCATACTTGATGGAGACCGGGACAGGATGCTGCAGGCAGGCTC GGGAGTGCATTATCGTTTCTTCTGAGCTCTTCATCCAGGCCATATTCCCTTTTCTGCATCGCATCTGGGG TGGGAGGGGCTGAGCTGGGAGTTTCCGTCCTTCTTCCCTGTGGGGGTGGGAGATGGGGCTCAAGGTCTCC ATCTCTCGGCTCCCTGAGGACAGAACCCCCACAGTGGACCTTTGGGCCTTCTCAGGACATTGACAATGTT GTGTGCACTGCAAGTTGACTTTATTTATTTTGTAGGAAAAAGAGATGGTATTCTTTAGCAGGATCTGAAA CTGTACCCTAGTCAAGAAGTACAATTAATAACATTATTATTAATAACAATTGGGATGATTCAAAGGTCAC ACACACACCAGACCACCCAAGAACCATGTAGGAGATGAGGCGGAGCCAGGTCATGGAAGCCCAGAATGCT ACAGAGATTCTGTGGGTCTATTATTGATCAGAAAATACAACATGGGGGGGCCTTTTCTATGGCATAACTC AGGTGTCTGCAAAGACAGAGCAGCTCAGGTGGGTGGATAGGCAGGCAGATGGAGCCTTGAATCACATCCC CTGAGGCTCAGCCAGCCAGTGCGAATCCTCAAGGTTCGGAAAGGGACAGTGTAGCGGCAAACTACTGGGC ATCTTCTCTGATGCTTAGAGGCTCTTACCAAGGAGCTTGATGGAGAAAGTGGCCATGTTGGTGGCATGGG ACAACTGCTCACCCAACTTCAAAGATCAAAGGGCACCCAGGTGGCCTATGACAGTGACACTCTTTCCTAC CATTAGGGGTGTCTGCCCCCACTGAAAGCCATGAAATTTCTGGCCCTAAGGGGGTAGGAAGGACTTAGGA GTAGCAGATGGTTTGATCCCATCCCCCCCACACACACACACACATCCCTCAGCTGTCTTCCACATTAGAG CCACTTCAGTTGTCCATGGACTTGTCCCTTTGAGACATCCTGGATTTTGAAGGATAGAAACATCCCCAAG ATGGTCTCGTGTTAATCCCACAACAGAGGCAGAAGGGTCATACTGAGAGAGAGAGAGAGAGAGAGAGAGA GAGAGAGAGGAGTGGGGGCCAGACCCTCAAGAAGCCAAGTGGGTCTGGTCAACCTGTGCACATGAGAAGG AGGGAACATCACTAAAATCAGGGCCTGGGCTGGTGTGTTGTTGGTGAGATCCCGTGGAGTGGCTGGCTAG ATATGGATGAGTTTTCTGAGCATGCTCACACACCCCCAACTTCACATTCTTAGAAATAGCACAACCATAA TGCCTTACCTCAAAAGGATGAGGCAAAGCTTGCAATTAATTCCCAGTGTCTGAAGACCTCCGGGATCCTT TGAGCTGTGTGTCCTTGTCAGTACATGGGGACAGGCTCCTTAGGTATTCCTGACATAGAGGTAAGGTGCT GCCCTTGCTGCCTTGCATAAGCTGTGACAAGCTTCTTACTGGGCACATAGAACGGCTCTGTCATCTGCTT CCACTAAATTTGGGCTTGGACTTTGCCTCCTGCCAAATCTCCATCTCTGCTGGATAGTCTAGTCCCTAGG GTCTAACCACCACCCTCCACTTCCTGGTGGGTCCTACAAGCGCTGTCTTTGTGCCAGTACCCGGATGGTG TCCTGCCTCATGGCTAAATGGTACAGGACATCTTCCCAGACTGAGTGGTCATGGCATGTGCATGTATGTT CATGAGTACATGCATGGTGTGTGGGGGTGGATGGGTGCATTCTGTTCTGCTTCTGGTGCAGCTACACAGC CACAACCTCTTTCTGTCATTGACCTTCTTGGTCTTCTTGTAGCCACAGATAATCTTCCAATGCCCGATTC TGCTGTTCTCATCTGAGAGCTGACAACCCAGCGCTCAGAGTAGAGTTCATGACCTAGGAAACCCTTCTCC TGGTAGCTTACTGAACTTATTTAATTAAAAACGAACATATAGGGGTTGGAGAGATGGTTCATTGGTTAAG AGCACCGGCTGCTTTTGCAAAGGACCTGAGTTCAAATCCCAGCAACCATATGGTGGCTTACAACCATCTG TAATGAGTTCTGGTGCCCTCTTCTGGCATGCAGGTGTACATGCAGATAGAGCACTCCTATGCATAAAATA AATAAATCTTTTAAATAAATAAATAAATGAACATGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6493234 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6493234 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14352 same embryo
	EMAGE:14356 same embryo
	EMAGE:14351 same embryo
	EMAGE:14355 same embryo
	EMAGE:14354 same embryo
	EurExpress:euxassay_004138 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source