EMAGE mouse gene expression spatial database

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EMAGE:14355

Rundc3b RUN domain containing 3B ( MGI:2685286)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004139. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004139_01	euxassay_004139_02	euxassay_004139_03	euxassay_004139_04

euxassay_004139_05	euxassay_004139_06	euxassay_004139_07	euxassay_004139_08	euxassay_004139_09

euxassay_004139_10	euxassay_004139_11	euxassay_004139_12	euxassay_004139_13	euxassay_004139_14

euxassay_004139_15	euxassay_004139_16	euxassay_004139_17	euxassay_004139_18	euxassay_004139_19

euxassay_004139_20	euxassay_004139_21	euxassay_004139_22	euxassay_004139_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14355_wholemount_strong.wlz

14355_wholemount_moderate.wlz

14355_wholemount_weak.wlz

14355_wholemount_possible.wlz

14355_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14355_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5613
Entity Detected:	Rundc3b, RUN domain containing 3B ( MGI:2685286)
Sequence:	sense strand is shown >T5613 CGGAGCCCGCCCTCCCGCCCGCCCGCCCGCGCGCGCTTCTCTCGTGGACGGCGGCGGCGGCGCGCGCGCG CGCGAGCGAGGGTGGAGGTGGCTCGTGCGTGCGTGTGAGTGAGCGCGTGTGTATGTGTGAGAGAGCGTGT GTGAGTGTGTGTGCACGCGCGCGTGGAGCCTGGGTACCGGTGCCCGACTGACTGCCTTCCACACCCTGCC TACTCCGCGCCTCCTTCCTCGCCTCTCTCCCGCCCGGCGGGCTGCCATCGTTGAGAGAAGGCCGGGGGAC ACAGGTGTGCGAGGCTTGCGGGACCGCTGGAGACAAAGGGGGCGCGGAGGTGAGCCCGCCATGGCCTCCC GGAGCCTGGGGGGCCTGAGCGGGAGCCGCGGCGGCGGCGGCGGCGGCGGCGGCAAGAAGAGCCTGAGCGC CCGCAACGCTGCGGTGGAGAGGAGGAACCTGATCACCGTGTGCAGATTTTCTGTGAAGACCCTGATTGAT CGGTCTTGCTTTGAAACAATTGATGATTCATCTCCTGAATTTAATAACTTTGCAGCTGTTCTGGAACAAA TTCTAAGTCACCGGCTAAAAGGTCAAGTAACATGGTTTGGCTATGAAAGCCCACGTAGCTTCTGGGACTA TATCAGAGTGGCTTGCCGGAAAGTTTCACAAAACTGTATCTGTAGTATTGAAAATATGGAAAATGTCAGC TCCTCCAGAGCTAAGGGTAGAGCCTGGATCAGAGTAGCACTCATGGAAAAACATTTGTCTGAATACATCT CTACAGCTTTGAGAGACTTCAAAACAACCAGGAGGTTTTATGAAGATGGAGCCATTGTCTTGGGTGAAGA AGCAAATATGCTTGCTGGCATGCTGCTTGGACTCAATGCTATTGACTTCAGCTTCTGCCTAAAGGGAGAA GGATTAGATGGCACCTTCCCTGCTGTGATAGACTATACACCATATTTGAAGTTTGAACAAAGTTCTGACA GCATCAGCAGTGACGAGGAGGAACTCAGGACGTTTGGGAGCAGTGACAGTGAGAGCAGCACCCCAGAGAA TGTGGGACCGCCTCTCATCCTAGACGAGAACACCTGGTTCAACAAGTGCAAGAGAGTAAGGCAGAAATAC CAGCTTACCCTGGAGCAGAAGGGTTATCTTGAAGAACTCTTGCGGCTTCGTGAGAACCAACTTTCTGAAT CTGTCTCTCAGAATAAAATCCTACTTCAGAGGATCGAAGATTCTGATCTGGCCCACAAATTGGAGAAAGA ACAGTTAGAATATATCATTGTGGAGCTTCAAGATCAGCTTAAAAGTTACCAAAGCCTTGACCAGTTATCA GCAGAAGTTAGCCTTTCTCAGGCCTCATTGGATCCCAGCCACTCACAAGAAGGAGATGGAAAGCAAGACT CATTGAATTTCATTGGTGAAGGTAAAGAAGATACCCCCTCACTTCTTGGCCTCTGTGGGTCCCTGACATC AGTGGCAAGTTACAAGTCCCTAACAAGCCTAAAATCCAACGACTGCCTTGCAAGCCCCACCACGGAGCTA ACCAGCCCAGGCCTGACTCCATCCTGAAAGACTACGTAAAAACCCAAAGTTTCTGCATTGTAAATATCCT GTTTACATAAGTTTGGCTGCTGGGAGATTTTGAAATTTTATATTCTGGTACATGCGTAACATTTTGTTGT TTGGAGATTTTAGTCCAGCCCATGTAAATTTCTGAGGGAAAAGCATAGTAGTCCCCTATTTTTCTTTTTA AAATATTACATTTGTCACTGAGGATGTTTAAAGTTGATTAGGCTTTAAAAATCTTTCAAAGAGTGTCTGA ATTCATTATAAATTGTACATAGTTGTTATTCAGCTGAATTTTTATATTTGTTTAAATGCGTCTTGGTGTC CAGAGACCAGGTCCAGGTTTTACTCCAGAGACTTAAGCTTAGCAGTTTCTTTCGCTCCTTTATTTGGTGG TTAAATTTGCACAAAAGGCAACTAATTCCATGAAGTACTTCCTGCACTCTGATGTCTGTGTACCATGCTG TAAGGGGAAGTCTCCAGCCACAACTACACAGTGGCTCGTTAAGCTTTCCTTTTACCAGTTGGTACCAACT TTTAGCATTTCATAAAGTTTGTAGAATTGTGCCTTTTTAGGCGGTTGCTGACTTAATTTGTCAGGTATGG AGGAAAAGCATCTTTTCCCAGGTATGCCTAAGACATTGGAAACATTTCAAACTTCAGAACTAATGTTGGC AAGGGGTTACTAACCTCAGAAGTTTCCAGTTTAATGAATGACTTGATAATGATTTTTAAAGAGCTGCAGA GTAACCAGGATGTCTGACTGTGGATATACATTGGAGCTACAATGCTATTTTCCCTGTGTGCAACAATGAC TTACATACTGGGTGTTTCATAGCCATTACCACTATGCCATGATTTCTCATTTTTCAAATTGATCTTAGTG TTTAAAAAAAAACAAAACTATACTTCTGTAGGAAAACAGGTTTTTTAAAAAGAGCTGTGTTTCAATTTTT TTTTTTACAATTTTTAAAACAGCTACATGTGTCCCTAAAAATTCCACCCTATTGAAAATAAACACGTATG AAGTGTGTATTCTATAGTGAGTCCTAGGAGGAAAACAAGTGTGCCAAAGACATTTCCTATACTGTTTCAT GAAGGGGCCACAGTGCTCACTCTTGTCTGTTGATTTTTATATTTAAACTTAGCAGCTGTGACAAGTAGGA AGTGCATCCCTCCTAAAGCTTCCTGGGGAGTGCTGAACTCACAGGTTCACTCCTTCTATCCAAAGTGTAG GGGTGGGAGGTTCTGGGAAGTGCTTGGAGCGCAGTCTCATGGTGTGGAATCAATCAATAGTAACATGACC AGTGTCACATGTGAGGCAGTGCAGACACTAATCTCTGGGGCAAAGGAGGCCTGCAAGCTCATGACAGACA TTGCATCGGGTGACTCAGAACAAACTGAGCAATTTCTTCCTGAGGCTGATGCATTAAAACAGAGCACCTC CATCTTCTCTGATCTGTGTGCACCATATTAAGTGTACAGTGGCCAAAGCCACGTTTAAGTAGTGTCAGGA ATAGCAAATAAAGACAGCAGGCCTGCAGCAGGCTGCATGAGGACTTAGATGGTCATGAGAACATTGGGAA TTTAGGCTGGCCGTGGGCGCACCTCTACTTTCCTAAGAAAGTCAAAGTGTTTACTGCTTTATCACCACGG CCTGCTCTCACCTGTCATTTATGATTTTTCAGATATAAAACCTTGGTAAGATCTTTTCATACATTTTTAT AAAGTAGAAGGGAATACAAGATTCCCCAGTTGGTACACTTTTTCAGATCTGTCCGATAGAAGCACTAGTT TGTGACATATCCCTGCTGCGAACTGCGGACTGAGACATGACACCTGAGGATCATGGCTTTCCCTCCATTA TTGGAGCTCTTGATATAAAGTTCATCTGCTACTGTGGAACCCAAGTTTCTGCAGTTGGTTTCGAGCTACA GGCAGTTTGTAACGCTTGCATGAGTGAACAGTGTCTCAGTCTTTAATTTTCAGTGGTACTGTTTATTTTT CAGAACTCCACAAAGAATGCTGGCTATGTCTATCTTTCCATCTGTATTTTGTTGCCTCGATGTACTATAT TTGTTGCATGTATATTAAAGATTTTTTTAACCATAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6491326 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6491326 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14352 same embryo
	EMAGE:14356 same embryo
	EMAGE:14351 same embryo
	EMAGE:14353 same embryo
	EMAGE:14354 same embryo
	EurExpress:euxassay_004139 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source