EMAGE mouse gene expression spatial database

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EMAGE:14372

Sdf2l1 stromal cell-derived factor 2-like 1 ( MGI:2149842)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004189. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004189_01	euxassay_004189_02	euxassay_004189_03	euxassay_004189_04

euxassay_004189_05	euxassay_004189_06	euxassay_004189_07	euxassay_004189_08	euxassay_004189_09

euxassay_004189_10	euxassay_004189_11	euxassay_004189_12	euxassay_004189_13	euxassay_004189_14

euxassay_004189_15	euxassay_004189_16	euxassay_004189_17	euxassay_004189_18	euxassay_004189_19

euxassay_004189_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14372_wholemount_strong.wlz

14372_wholemount_moderate.wlz

14372_wholemount_weak.wlz

14372_wholemount_possible.wlz

14372_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14372_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 13 14

diencephalon lateral wall ventricular layer

moderate

homogeneous

moderate expression: see section 12 13 14 15

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 10 14 15

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

medulla oblongata basal plate ventricular layer

moderate

homogeneous

moderate expression: see section 10 11 13 14 15 16 17 18

rest of cerebellum ventricular layer

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11

pons ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 13 14 15 16 17 18 19

midbrain ventricular layer

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15 16 17 18 19

spinal cord ventricular layer

moderate

homogeneous

moderate expression: see section 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7879
Entity Detected:	Sdf2l1, stromal cell-derived factor 2-like 1 ( MGI:2149842)
Sequence:	sense strand is shown >T7879 GTTGAGGACGGAAGCGCAGAGGTCTAGAGCAGCTGGAACCTGGCCGGCTGGCGGGATGTGGGGCGCGAGC CGGGGCAGGGTAGCGGGGCCAACGCTGCTGGGGCTGCTGCTGGCGCTCTCAGTGCGGAGTGGGGGCGCGT CCAAGGCCAGCGCCGGGCTAGTGACCTGCGGGTCAGTGCTGAAGCTGCTCAACACCCACCACAAAGTGCG GCTGCACTCACACGACATCAAATACGGATCTGGCAGCGGCCAACAGTCGGTAACCGGCGTGGAGGAGTCC GACGATGCCAATAGTTACTGGCGGATTCGCGGCGGCTCCGAGGGTGGGTGCCCGCGCGGACTCCCAGTGC GCTGTGGGCAAGCAGTGCGGCTCACGCACGTGCTCACCGGCAAGAACCTGCACACGCACCACTTCCCGTC GCCGCTATCCAACAACCAGGAGGTGAGTGCTTTTGGGGAAGACGGTGAGGGTGATGACCTGGACCTGTGG ACAGTCCGATGTTCTGGGCAACACTGGGAGCGAGAGGCCAGTGTCCGCTTCCAGCACGTGGGCACCTCTG TGTTCCTGTCGGTCACTGGTGAACAGTATGGTAACCCCATCCGTGGGCAGCATGAGGTTCATGGCATGCC TAGTGCCAATGCTCACAACACGTGGAAGGCAATGGAAGGAATCTTCATCAAGCCTGGAGCAGATCTCTCC ACGGGTCACGATGAACTCTGAGAACCAGATGAGTGATGGGAGGGTGGCTGAGTGGCAATCTCGGGGTCAC TCTTGTGTAAGACTTTGTTTGTAGGGGCCCTCAAGTGCCTTTATGATTAAAGAATGTTGGTCTGGGATTA TTTTTGCTGTAACCCTGGGGAAGATCTGAAGGTGCCAGCCATATCTGTCCAAGTTACCATCTCACCTGTC TCAAGCACCTGCTGAAATTTTTTTTTTTTTTTTTTTTTGAGACTGTCTCACTATGTATCCTTGGCAGGCC TGGAACTCACAGAGAGATCCACCTGCCTCTGTCTCCTGAGTGCTGGTATTAAAGGTGTACACCACCACAA CTGGCCCCAACCTGTTCAATAAACTCATTTTTATTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6506821 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6506821 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14371 same assay
	EMAGE:14375 same embryo
	EMAGE:14373 same embryo
	EMAGE:14374 same embryo
	EMAGE:14369 same embryo
	EurExpress:euxassay_004189 same experiment
	EMAGE:14370 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source