EMAGE mouse gene expression spatial database

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EMAGE:14373

Txnrd2 thioredoxin reductase 2 ( MGI:1347023)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004191. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004191_01	euxassay_004191_02	euxassay_004191_03	euxassay_004191_04

euxassay_004191_05	euxassay_004191_06	euxassay_004191_07	euxassay_004191_08	euxassay_004191_09

euxassay_004191_10	euxassay_004191_11	euxassay_004191_12	euxassay_004191_13	euxassay_004191_14

euxassay_004191_15	euxassay_004191_16	euxassay_004191_17	euxassay_004191_18	euxassay_004191_19

euxassay_004191_20	euxassay_004191_21	euxassay_004191_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14373_wholemount_strong.wlz

14373_wholemount_moderate.wlz

14373_wholemount_weak.wlz

14373_wholemount_possible.wlz

14373_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14373_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

strong

regional

strong expression: see section 13 14 15 16 17 18 22 weak expression: see section 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7876
Entity Detected:	Txnrd2, thioredoxin reductase 2 ( MGI:1347023)
Sequence:	sense strand is shown >T7876 CCGGACCATGGCGGCGATGGTGGCGGCGATGGTGGCGGCGCTGCGTGGACCCAGCAGGCGCTTCCGGCCG CGGACACGGGCTCTGACACGCGGGACAAGGGGCGCGGCGAGTGCAGCGGGAGGGCAGCAGAGCTTTGATC TCTTGGTGATCGGTGGGGGATCCGGTGGCCTAGCTTGTGCCAAGGAAGCTGCTCAGCTGGGAAAGAAGGT GGCTGTGGCTGACTATGTGGAACCTTCTCCCCGAGGCACCAAGTGGGGCCTTGGTGGCACCTGTGTCAAC GTGGGTTGCATACCCAAGAAGCTGATGCATCAGGCTGCACTGCTGGGGGGCATGATCAGAGATGCTCACC ACTATGGCTGGGAGGTGGCCCAGCCTGTCCAACACAACTGGAAGACAATGGCAGAAGCCGTGCAAAACCA TGTGAAATCCTTGAACTGGGGTCATCGCGTCCAACTGCAGGACAGGAAAGTCAAGTACTTTAACATCAAA GCCAGCTTTGTGGATGAGCACACAGTTCGCGGTGTGGACAAAGGCGGGAAGGCGACTCTGCTTTCAGCTG AGCACATTGTCATTGCTACAGGAGGACGGCCAAGGTACCCCACACAAGTCAAAGGAGCCCTGGAATATGG AATCACAAGTGACGACATCTTCTGGCTGAAGGAGTCCCCTGGGAAAACGTTGGTGGTTGGAGCCAGCTAT GTGGCCCTAGAGTGTGCTGGCTTCCTCACTGGAATTGGACTGGATACCACTGTCATGATGCGCAGCATCC CTCTCCGAGGCTTTGACCAGCAAATGTCATCTTTGGTCACAGAGCACATGGAGTCTCATGGCACCCAGTT CCTGAAAGGCTGTGTCCCCTCCCACATCAAAAAACTCCCAACTAACCAGCTGCAGGTCACTTGGGAGGAT CATGCTTCTGGCAAGGAAGACACAGGCACCTTTGACACTGTCCTGTGGGCCATAGGGCGAGTTCCAGAAA CCAGGACTTTGAATCTGGAGAAGGCTGGCATCAGTACCAACCCTAAGAATCAGAAGATTATTGTGGATGC CCAGGAGGCTACCTCTGTTCCCCACATCTATGCCATTGGAGATGTTGCTGAGGGGCGGCCTGAGCTGACG CCCACAGCTATCAAGGCAGGAAAGCTTCTGGCTCAGCGGCTCTTTGGGAAATCCTCAACCTTAATGGATT ACAGCAATGTTCCCACAACTGTCTTTACACCACTGGAGTATGGCTGTGTGGGGCTGTCTGAGGAGGAGGC TGTGGCTCTCCATGGCCAGGAGCATGTAGAGGTTTACCATGCATATTATAAGCCCCTAGAGTTCACGGTG GCGGATAGGGATGCATCACAGTGCTACATAAAGATGGTATGCATGAGGGAGCCCCCACAACTGGTGCTGG GCCTGCACTTCCTTGGCCCCAACGCTGGAGAAGTCACCCAAGGATTTGCTCTTGGGATCAAGTGTGGGGC TTCATATGCACAGGTGATGCAGACAGTAGGGATCCATCCCACCTGCTCTGAGGAGGTGGTCAAGCTGCAC ATCTCCAAGCGCTCCGGCCTGGAGCCTACTGTGACTGGTTGCTGAGGCTAAGTCACCATCCCTGCTGAGC TAAGGACACACAGTGTGCCATGTGCCCAATAGAAGGCTCCTCAGACACCTGGATCCAGGACTGGAAGGCC TTGCTGCCTCTCAGAGATCTGAGAAGATGTGGATGGAACATCGTGTGTCCTGCAGGGATGACTATACCCC TACATTCTCTGGCCAGCCTCTGACACTCCCAGCGTCAGATGATGATGGCCTGGGCAGAAACCCCATGTGG GCCGCCCAGGTTTGAACCCCTGGCATTTCTAGAGCACTAATAAAGAGGATGTTTTACTAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6506286 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6506286 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14375 same embryo
	EMAGE:14371 same embryo
	EMAGE:14372 same embryo
	EMAGE:14374 same embryo
	EMAGE:14369 same embryo
	EMAGE:14370 same embryo
	EurExpress:euxassay_004191 same experiment
	MGI:4829036 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source