EMAGE mouse gene expression spatial database

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EMAGE:14387

Gadd45g growth arrest and DNA-damage-inducible 45 gamma ( MGI:1346325)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004217. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004217_01	euxassay_004217_02	euxassay_004217_03	euxassay_004217_04

euxassay_004217_05	euxassay_004217_06	euxassay_004217_07	euxassay_004217_08	euxassay_004217_09

euxassay_004217_10	euxassay_004217_11	euxassay_004217_12	euxassay_004217_13	euxassay_004217_14

euxassay_004217_15	euxassay_004217_16	euxassay_004217_17	euxassay_004217_18	euxassay_004217_19

euxassay_004217_20	euxassay_004217_21	euxassay_004217_22	euxassay_004217_23	euxassay_004217_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14387_wholemount_strong.wlz

14387_wholemount_moderate.wlz

14387_wholemount_weak.wlz

14387_wholemount_possible.wlz

14387_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14387_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14 not examined expression: see section 06 07 08 09 10 11

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 15 16

4th ventricle

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

midbrain ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6111
Entity Detected:	Gadd45g, growth arrest and DNA-damage-inducible 45 gamma ( MGI:1346325)
Sequence:	sense strand is shown >T6111 GGCGCGCATCGGACTCTGGGAATCTTTACCTGCGCTCCGGTTCCCTCCGCACTCTTTTGGATAACTTGCT GTTCGTGGATCGCACAATGACTCTGGAAGAAGTCCGTGGCCAGGATACAGTTCCGGAAAGCACAGCCAGG ATGCAGGGCGCCGGGAAAGCACTGCACGAACTTCTGCTGTCGGCGCAGCGCCAGGGCTGTCTGACCGCTG GCGTCTACGAGTCCGCCAAAGTCCTGAATGTGGACCCTGACAATGTGACCTTTTGCGTGCTGGCTGCCGA TGAAGAAGATGAGGGCGACATAGCGCTGCAGATCCATTTCACGTTGATTCAGGCGTTCTGCTGTGAGAAC GACATTGATATCGTGCGCGTGGGAGACGTGCAGAGGCTGGCGGCGATTGTGGGCTCCGACGAAGAGGGGG GCGCGCCGGGAGACCTGCATTGCATCCTCATTTCGAATCCTAATGAGGACACATGGAAGGACCCTGCCTT GGAGAAGCTCAGTTTGTTCTGCGAGGAGAGCCGCAGCTTCAACGACTGGGTGCCCAGCATCACCCTTCCC GAGTGACAGCCTGGCAGGGACCTTGGTCTGATCGACTTGGTAACACTCTAGCGCGCTGCTGGCTCTGGAG TGGCCCTCCGAGGGCGCTCGAGTGCGCATGGAGACTGGCAGGCGATGTTGCCTGGAGAGCGAGGAGCGCG ACCTCCCAAGAAGGGGGTCTGGCGGCAGCGGGGACACCTTGTTCCGAGCCCAGGACTCTGCCAGTGTCCG GAGAGGCTGCTAGCACAGGAAGGCCTAGGCGAGGACGTTGGCCCCAGGGCCGGGAAGAACCGACCAGCGA GGCAGGTGTGACTCAGCAAGCAGCCTTCCAGTGAAAGGAGGGGAAAGAAAGGCAGGCGACCGCCTGGACT TGGTACAGCGGCAGGAGCGGCCACTGCAGGAGCGAGCTGGACTTAGCCGACTGCACTGCTCTTTCAAAAA ACGGATCCCGGGCAATGCTTTCATTTTCTAAAGGACGCTATCGTGGAAGCTTTGAATATCACAATAAACT TATTGAAACAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3493618 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3493618 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14386 same embryo
	EMAGE:14390 same embryo
	EMAGE:14388 same embryo
	EMAGE:14389 same embryo
	EurExpress:euxassay_004217 same experiment
	MGI:4824974 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source