EMAGE mouse gene expression spatial database

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EMAGE:14392

Mmp15 matrix metallopeptidase 15 ( MGI:109320)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004220. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004220_01	euxassay_004220_02	euxassay_004220_03	euxassay_004220_04

euxassay_004220_05	euxassay_004220_06	euxassay_004220_07	euxassay_004220_08	euxassay_004220_09

euxassay_004220_10	euxassay_004220_11	euxassay_004220_12	euxassay_004220_13	euxassay_004220_14

euxassay_004220_15	euxassay_004220_16	euxassay_004220_17	euxassay_004220_18	euxassay_004220_19

euxassay_004220_20	euxassay_004220_21	euxassay_004220_22	euxassay_004220_23	euxassay_004220_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14392_wholemount_strong.wlz

14392_wholemount_moderate.wlz

14392_wholemount_weak.wlz

14392_wholemount_possible.wlz

14392_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14392_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 07

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 17 18 19 20

cervico-thoracic ganglion

weak

regional

weak expression: see section 09 15

cervical ganglion

weak

regional

weak expression: see section 08 09 16 17

dorsal root ganglion

weak

regional

weak expression: see section 07 08 09 10 11 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7850
Entity Detected:	Mmp15, matrix metallopeptidase 15 ( MGI:109320)
Sequence:	sense strand is shown >T7850 CTCTGTCGGGCCGGTTCCCTGCGCCCCTCCCGGGACTGCCTGGGGATCCAGGCCCGATTGGGCGCGGCAG CTAGTGGAGTCGCCAGAGGGAAGCGCGCACTCTGGGCAGCGCGGAGGACCTTGGGCATGGACAGAGAGTG CAGGGCGCGCAGGGGGCGCCGCGGGACCGGGCGGCCGGAGCGCTGAGCCAGACAAAGGCTCTGGAGTTCT GCTTGCTCTCGGGGGCCGGGGCCAGGGCGCACCGCGCCGACGCTGCGAGACTCTGCGGCGTGCAGCTCGG CCGGTCCCCTTCTCCAGCGGGCGAGTCCCCGCGTGCCCCGACCTGCCCTGCCCGCTTCTCACCCGGTTTG GGAATTGGCAGAGGGGACCTGAGCTCCTCAGGCAGGCACCGGGAGCCGGAGGTCCGCCGCGCGCCCGCGG GGCCGGGAGCCAGGGAGCGTCGCAAGTTTCCCAGCCGCGTGCGAGGACGCGGTGCGGGCCAGCCAGCTAG CCCCGCGTGCAGCGCTCAGGACCGGGCTGACTGCCTAGGAGCATGGGCAGCGACCGGAGCGCACTCGGAC GTCCAGGCTGCACTGGCAGCTGCCTCAGCAGCCGAGCTTCGCTGCTCCCGCTGCTACTGGTGCTTCTGGA CTGTCTGGGCCATGGTACAGCGTCTAAAGACGCCGAAGTGTACGCCGCGGAGAACTGGCTGCGGCTCTAT GGCTACCTACCCCAGCCCAGCCGCCACATGTCCACCATGCGCTCTGCCCAGATCCTGGCCTCCGCCCTTG CCGAGATGCAGAGTTTCTATGGGATCCCTGTCACGGGTGTGCTTGATGAAGAGACGAAAACGTGGATGAA GCGGCCCCGATGTGGGGTTCCTGATCAGTTTGGGGTACATGTGAAAGCCAACCTGCGTCGACGGCGGAAA CGTTACACCCTGACAGGAAAGGCATGGAACAATTACCACCTGACCTTCAGCATCCAGAACTACACTGAGA AGCTGGGCTGGTACAACTCCATGGAGGCGGTGCGCAGGGCTTTCCAAGTGTGGGAGCAGGTCACACCATT GGTCTTCCAGGAAGTATCCTATGATGACATTCGGCTACGAAGGCGAGCGGAGGCTGACATCATGGTACTC TTTGCCTCTGGCTTCCATGGCGACAGCTCACCGTTTGATGGCGTGGGTGGCTTTCTGGCCCACGCTTATT TCCCCGGCCCTGGTCTGGGTGGGGACACCCATTTCGACGCAGATGAACCCTGGACCTTCTCCAGCACTGA CCTGCATGGAATCAGCCTCTTTCTGGTGGCCGTGCATGAGCTGGGCCATGCCCTGGGGCTAGAACACTCA AGTAACCCCAGCGCTATTATGGCACCCTTCTACCAGTGGATGGATACCGACAACTTCCAGCTGCCCGAAG ATGACCTTCGGGGCATCCAGCAGCTGTATGGCTCCCCAGATGGTAAGCCACAGCCCACCCGGCCTCTCCC CACTGTGAGGCCCCGGCGGCCAGGACGGCCAGATCACCAGCCACCTCGGCCTCCCCAGCCACCACATCCA GGTGGGAAGCCAGAAAGGCCCCCCAAACCAGGGCCCCCACCCCAGCCCCGAGCCACAGAGAGGCCTGACC AGTATGGCCCCAACATCTGCGATGGCAACTTCGACACAGTGGCTGTGCTCCGCGGAGAGATGTTTGTGTT CAAGGGCCGCTGGTTCTGGCGAGTCAGGCACAACCGCGTTCTAGACAACTACCCCATGCCAATTGGCCAC TTCTGGCGCGGTCTGCCGGGGAACATCAGTGCTGCCTACGAGCGCCAGGATGGACATTTTGTCTTCTTCA AAGGTAACCGCTACTGGCTTTTCCGAGAAGCCAATCTGGAGCCCGGCTACCCACAGCCGCTGAGCAGCTA TGGCACAGACATCCCCTATGACCGCATTGACACAGCCATCTGGTGGGAGCCCACAGGTCACACCTTCTTC TTTCAAGCAGACAGGTACTGGCGCTTCAACGAGGAGACACAGCATGGAGACCCTGGCTACCCCAAGCCCA TCAGTGTCTGGCAGGGGATCCCCACCTCTCCCAAAGGGGCCTTCCTCAGCAACGATGCAGCCTACACCTA CTTCTACAAGGGCACCAAGTACTGGAAATTCAACAACGAACGCCTACGGATGGAACCCGGCCACCCCAAA TCCATCCTGCGGGACTTCATGGGCTGCCAGGAGCACGTGGAGCCCCGATCGCGATGGCCCGATGTGGCTC GTCCACCCTTCAACCCCAACGGGGGTGCCGAGCCTGAGGCAGATGGTGACAGCAAGGAAGAGAACGCGGG TGACAAGGATGAGGGCAGCCGCGTGGTGGTGCAGATGGAGGAGGTGGTCCGGACAGTGAACGTGGTGATG GTGTTGGTCCCCTTGCTGCTGTTGCTCTGTATTCTGGGCCTGGCCTTTGCTCTGGTGCAGATGCAGCGCA AGGGCGCCCCTCGCATGCTGCTCTACTGCAAGCGCTCACTGCAGGAGTGGGTGTGATCAGGTAGCACCCG CAGCACCCGGTGCTCAGGTCTGCAGGGACAGGAGACGTGTGGATCCCAGATGCTCCCTCGGCGCGGCAGG ATGCCCTCCTAGCCACACACATCCCCCTGCCAGCCTTGCCCCCCACCCCCGTCCTCCTTTATTTATGCCC AGGTGCCTCTTCTTTTTGGCACCCTCTCTCAGCCTTTGGTTCCGCTTTCCTGACTCGGGCCAGGAGATGC TTTGAGATCTCCCGGAGTGTAGTACCGCCCCAGGCAGGAACGAAGGGACAGAGGCTCACACCTCTGGAAG ATGGCAGAGGGCTGGGAAGCTTCTCTGGCTCTGGCTTTTCAGCCTGAACCTCTCCTCCCACGACAAGAAC TGTGTTTGCCTTGAAGGAGCCCACCTCGGGCTCTGCCCTCCTGAATTTGCCTTAGGGAGAGTATATGCTC CCCCCCCTGGGGGGGGCACCCTGATAACCTTGAGACTTGGTTCCTTCTTCTTAGGCTCCTAAGAAGCCCA GAAGCCTAAAAGCCCTACCCCATCCTAGCATTCTGCAGCTCCTACTTGCCCCGGGCCTGCAGCTTCTTTC CTCTAGGAAGGGGCCCTGGACATGTCTCACCTAGGACCAAAGGGAGCCTGCCAAGCTCCCCTCCCAATGC TAGTGACTAGCTCCTCCTTCCCTAGGCTGCCCATATTCTCTTTCAGTGCCCCATAGGTCTCCGGAGGCTG GGCACTCCCCGCCCGCCCCCACCCCCAGCTTCTGCAGTCCTTGTCCCAACTCTTAATGACATGGGGCAGA CACACCCACTAGGGTACCAGAGGTCCCTGGGCAGTGTGTGTGATTGTGGATGCTCAGCAGGTCACAGACC AAGCTTATGGGCTTCCTCCCTTCCAGATTCCTGTCCCCACACAACACTACTAGGCTCAGGCCAGCAGCTC ACCCCACACAGAGTCTGTGGAACCCCCAAAGGAATCCACTAAGGCCTGGGAAGTCTGTCCTAAGGGTGAA AGGGGCCCTCCCTTCCTCTTCTCATCCTGGAGTCAGCAGGAGGCTTCTGTGGGAAATGGTACTGTACAGC TGTGGCTCTGTCTCCTCTGCCTCCCTAAAGCCTTGAGCAGTAAAGGGCTGCCAGCCAGGATCCTCTGGGT CACCGCCGCTTTCCAAGTGGTGGATTCTTATAGCCTGGCACTAGAGGTGACAGATCGGAGGGGCATGTCC TGCAGCACTCTAGATGTCCCCTCTGCAGAACCTGAGAGTCAGCAAGGGGTGGGGCGTCCACCCCCCCACT CCCCATCGTTTAGTTCTGTAGATAATCAGCACTGATTAAATGTCCAGCCAGCGAGCGTGGCTGCACTTGT ACGCTCAGACCCAAGCATGCCGCAAGGAAGACCTGCTTGGCAGGGTCGGGGCCAAGCGAGGATGCCGAGG AATACCCTCCTCCCAGTTTGGTAGGACCTTTGGGATGGAGTCCAGGAAGGTCAGGGAAGAGTGGGGGGGG GGGGGCAGTAGGAGTCTGGTTGGGCTGAGGTTTACTGGGTTTACTTAACATTTGGTAGTGATAAGAGGAG AGGACAGCCCAGCTTCCCAAATGACTCCACATCTGGCGGTCTGGAAGAACCAGACAAGGCCGCCCAATCG TCCCACCCACCTGGATTGGATTTTGGGCAGCCTGAGAGGTGGCTTGAACAAGAACTCAGGGATGGGTCTG GAGAGAATTGTGTAACGGGGCCACTTTACCTCCAGAATCTTCCCTCCATGTTCAGAGAATAAAATGCACT GGAACCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6390113 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6390113 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14393 same embryo
	EMAGE:14391 same embryo
	EMAGE:14395 same embryo
	EMAGE:14394 same embryo
	EurExpress:euxassay_004220 same experiment
	MGI:4826406 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source