EMAGE mouse gene expression spatial database

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EMAGE:14393

Adam9 a disintegrin and metallopeptidase domain 9 (meltrin gamma) ( MGI:105376)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004213. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004213_01	euxassay_004213_02	euxassay_004213_03	euxassay_004213_04

euxassay_004213_05	euxassay_004213_06	euxassay_004213_07	euxassay_004213_08	euxassay_004213_09

euxassay_004213_10	euxassay_004213_11	euxassay_004213_12	euxassay_004213_13	euxassay_004213_14

euxassay_004213_15	euxassay_004213_16	euxassay_004213_17	euxassay_004213_18	euxassay_004213_19

euxassay_004213_20	euxassay_004213_21	euxassay_004213_22	euxassay_004213_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14393_wholemount_strong.wlz

14393_wholemount_moderate.wlz

14393_wholemount_weak.wlz

14393_wholemount_possible.wlz

14393_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14393_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5594
Entity Detected:	Adam9, a disintegrin and metallopeptidase domain 9 (meltrin gamma) ( MGI:105376)
Sequence:	sense strand is shown >T5594 ACCTGCCGAAGCCCTCGCTATGGGGCCGCGCGCGCTCTCGCCCCTTGCCTCTCTGCGACTAAGGTGGCTG CTGGCGTGTGGCTTGCTGGGCCCAGTCCTCGAGGCCGGGCGACCAGACTTGGAACAGACTGTCCATCTTT CTTCTTATGAAATTATTACTCCTTGGAGATTAACTAGAGAAAGAAGGGAAGCTCTGGGGCCCAGTTCACA GCAGATCTCTTACGTCATCCAGGCCCAAGGAAAACAGCATATTATTCACTTGGAAAGAAACACAGACCTT TTACCTAATGATTTTGTAGTTTACACCTACGACAAGGAAGGCTCCCTACTCTCTGACCATCCCAACGTAC AGAGCCATTGTCACTATCGAGGCTATGTGGAGGGAGTGCAGAATTCCGCGGTTGCTGTGAGCGCCTGCTT TGGACTCAGAGGCTTGCTGCATTTGGAGAATGCCAGTTTTGGAATTGAACCTCTGCACAACAGCTCACAC TTTGAGCACATATTTTACCCCATGGATGGCATCCACCAGGAGCCTCTGAGATGTGGAGTCTCTAACAGGG ACACAGAGAAGGAAGGCACACAGGGGGATGAGGAGGAGCATCCGAGTGTCACTCAGCTGCTGCGCAGAAG AAGAGCTGTTCTACCACAGACCCGCTATGTGGAGCTGTTCATTGTTGTAGACAAGGAAAGGTACGACATG ATGGGACGGAACCAGACTGCTGTGAGAGAAGAGATGATTCGCTTAGCAAACTACCTGGATAGCATGTACA TCATGTTAAACATTCGAATTGTGCTGGTTGGACTAGAAATTTGGACAGACAGAAATCCTATCAATATAAT TGGAGGAGCTGGAGATGTGCTGGGCAACTTTGTTCAGTGGCGGGAAAAGTTCCTTATAACTCGTCGGAGA CACGACAGTGCACAGTTGGTTTTGAAGAAAGGCTTTGGTGGAACTGCAGGAATGGCGTTTGTAGGAACAG TATGTTCAAGGAGCCACGCAGGTGGGATCAATGTGTTTGGGCAAATCACTGTGGAGACATTTGCATCCAT TGTTGCTCATGAATTGGGGCATAACCTTGGAATGAATCATGATGATGGGAGAGAGTGTTTCTGTGGAGCA AAGAGCTGTATCATGAATTCAGGAGCATCCGGGTCCAGAAACTTTAGCAGTTGCAGTGCGGAGGACTTTG AGAAGTTAACGTTGAATAAGGGAGGAAGCTGCCTGCTTAACATCCCGAAGCCTGACGAAGCCTACAGCGC GCCCTCCTGTGGTAATAAGCTGGTGGACCCTGGAGAGGAGTGTGACTGCGGCACAGCGAAGGAGTGTGAG GTGGACCCATGCTGTGAAGGAAGCACTTGTAAGCTCAAGTCATTTGCTGAGTGTGCATATGGCGACTGTT GTAAAGATTGCCAGTTCCTTCCAGGAGGCTCCATGTGCAGAGGGAAGACCAGTGAGTGTGATGTTCCTGA GTACTGCAACGGTTCCTCTCAGTTCTGCCCGCCAGATGTCTTCATTCAGAATGGATATCCTTGCCAGAAC AGCAAAGCCTACTGCTACAATGGCATGTGCCAATATTATGACGCGCAGTGTCAGGTCATCTTTGGTTCAA AGGCTAAGGCTGCCCCAAGAGATTGCTTCATTGAAGTCAATTCTAAAGGTGACAGATTTGGCAACTGTGG TTTCTCCGGCAGTGAGTACAAGAAGTGTGCCACTGGGAACGCGCTGTGTGGAAAGCTTCAATGCGAGAAT GTACAGGACATGCCGGTGTTTGGAATAGTACCAGCTATCATTCAGACACCCAGTCGAGGCACCAAATGCT GGGGTGTGGATTTCCAGCTTGGTTCCGACGTTCCAGACCCAGGGATGGTGAATGAAGGCACCAAATGTGA TGCTGGCAAGATTTGCAGGAATTTTCAGTGTGTAAATGCTTCTGTCCTGAATTATGACTGTGACATTCAG GGAAAATGTCATGGCCATGGGGTATGTAACAGCAATAAGAATTGTCACTGTGAAGATGGCTGGGCTCCCC CACACTGTGACACCAAAGGATATGGAGGAAGCGTGGACAGCGGGCCGACGTATAATGCAAAGAGCACAGC ACTGAGGGACGGGCTTCTGGTCTTCTTCTTCCTAATCGTCCCCCTTGTTGCGGCTGCCATTTTCCTCTTT ATCAAGAGAGATGAACTACGGAAAACCTTCAGGAAGAAGAGATCACAAATGTCAGATGGCAGAAATCAAG CAAACGTCTCTAGACAGCCAGGAGATCCTAGTATCTCCAGACCACCAGGGGGCCCAAATGTCTCCAGACC ACCAGGGGGCCCAGGTGTCTCCAGACCACCAGGGGGCCCAGGTGTCTCCAGACCACCAGGGGGCCCAGGT GTCTCCAGACCGCCACCTGGGCATGGAAACAGATTCCCAGTACCAACCTACGCCGCCAAGCAGCCTGCGC AGTTCCCGTCAAGGCCACCTCCACCACAACCGAAAATATCTTCTCAGGGAAACTTGATTCCGGCTCGGCC CGCTCCTGCACCTCCTTTATATAGCTCCCTCACCTGATAGTAGAATATTAGAATCTTATTTTTTAAATGT CTTCAGGGAACTGAGCAAATGTTTGTTGTTTTTTTTTTTCCTGATGTTTTCTTGAAAAGCCTTTCTCTTC CAACCATGAATGAACACAAACCACCACAAAACAAGCTTTATTAACACAGGAGCCTAGTGGGGATTGCGAA ACACAGGAATGTGCAGGCGCTCCGGGGGGTGTAAAGTGAACGTTTCCATCGTTAGAATGTTTTCTCTGGC CATTTGTGGATTTAATGCACTTGACGTGGATTAAGTTATTCTGAGCATGTTACTGTAATGATTCTCAAAT TAACTGTATTAGTGTAAGCTTTGTCACTATGCGCTAAACGTAATCCTGACTTTTTGACCCCAGTTACCAT TAATAGTTTCTGGTTGACCATTTGAATATGTATTAACTTAGGAAGACTAATTGCCAATAACGTCTGCATT TTCATCTTGCATGGATTAACAGCCATTTATATGGACTTATGTCTCTTAATGCACAAAGAAGCAGATATCT CGAAGGAGCTTACACAAGAACCACAATTACTAGATCATGATATACTTGGAAAGTGTGAAATATGGTGTGT ACTCAGTTATTGGCTTCCATTTTTTATGATCTTTCAACTATAACAATTATGATAGAAATCGATTTAACAC AATCAGTTATGGGCTTCCATTTTCAAATATCTTTTCAACTGTAATGATTATGACAGGAACTGATTCAACT CTCAATTTTCTTTATGCATCATGGTAAAGCATTGCAGCAGTGTTGTTTTGTTTGAAGTGCACACTCTATG GTACGAGGTGTTTAGTATACCCAAGCAGATAGGTGTCGATCGAACAGGAGCAGGGAGAATACTTCCAACA GTTGAGGTGTTACCAAACCACTTGAGAATTCATGAGCACTTTAACTCTAAACTCTGAATTTCAAAGCTTG ATGTGAAGTCCTCTAGAATGTTTACATTTACTAAGGTGTGCTGGGTCCTGTCTCTTTTGACTAATATTTT CGTAAACATTAGGCTGGAGAAAGGAAGGAAGCAGTGGTTTCCTTAGATAACTACAGAATTATACTGGTCT CTGGGATTACTCTCTCAGCTGTATTAAAATGAATTTGTACTTTGAAAGGAATGATATTGACACTAAAATT TTAAACATTTAAATTTTTTCATAATCTTTCATAAAGAAGTTTAATAATAGGTATATTAACTGAATTTCAT TAGTTTTTTAAAATAATATTGTTTGTGTATATATACATATTAAAATAAAAACATTTACAACAAATAAAAT ACTTGAAATTCTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6488707 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6488707 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14391 same embryo
	EMAGE:14395 same embryo
	EMAGE:14392 same embryo
	EMAGE:14394 same embryo
	EurExpress:euxassay_004213 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source