EMAGE mouse gene expression spatial database

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EMAGE:14395

Emilin2 elastin microfibril interfacer 2 ( MGI:2389136)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004216. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004216_01	euxassay_004216_02	euxassay_004216_03	euxassay_004216_04

euxassay_004216_05	euxassay_004216_06	euxassay_004216_07	euxassay_004216_08	euxassay_004216_09

euxassay_004216_10	euxassay_004216_11	euxassay_004216_12	euxassay_004216_13	euxassay_004216_14

euxassay_004216_15	euxassay_004216_16	euxassay_004216_17	euxassay_004216_18	euxassay_004216_19

euxassay_004216_20	euxassay_004216_21	euxassay_004216_22	euxassay_004216_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14395_wholemount_strong.wlz

14395_wholemount_moderate.wlz

14395_wholemount_weak.wlz

14395_wholemount_possible.wlz

14395_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14395_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7896
Entity Detected:	Emilin2, elastin microfibril interfacer 2 ( MGI:2389136)
Sequence:	sense strand is shown >T7896 GTTCAAGCCGTGTACTAGCTGCACTTCCGGAGGGGCTGGGGTCGCGCTGTTTGGAAGGTGAGAGGCGGAG CTGCTGGCGTTTGGGAAGAGACCAAAGGAATGTGTAATTGAAAAGTCTGTAACCAAGACCCCGGTTCCTA ACCCTTTGGCTCTTTCCCCGAACCCCCTGCCGAGAGGCGATGGGCCGAGAGATGGTGAAGGGAAGAGTCA CGTTTCGGAGAGGGAAGTGAATTAAAGAGGGCGGCACCCGGGGGGACCTGTGCGTCATTGAGGGACCGCG GAGCGGCCAGTGCGGAGGCGTGGGGACGAAGAGCCAGAGGGGCCGCCGAGCGCCCGAGCCCGCTGCCGGT TCCCGCGGCCCCGCCGCCTGCAGCCTTGTGGGGCTCAAGATGTGTCAGGAGACCCCGCCCCGACCCCGAG CACCCAGTCGCTGGACGCCGGCCCTGCTGGCCCTGCTGGCCCTGGGCGGCGCGGGACTGTGCCACGCGAG CTCGCAGCCTGGGTACCACGCGAGGCCCAGTGCCAGGAACAAAAACTGGTGCGCCTACATCGTGAATAAG AACGTGAGCTGCACGGTACAGGAGGGAAGCGAGAGTTTTATTCAAGCTCAGTACAACTGTCCCTGGAACC AGATGCCCTGTCCATCCGCCCTGGTGTATCGGGTAAACTTTAGACCCAGATTTGTCACTAGATACAAGAT AGTGACACAGTTGGAATGGAGATGCTGTCCTGGCTTTAGAGGACCAGACTGCCAAGAAGGTCCCAAAGAC CACATGAAGACCCCCCGGCCCCCATCAGCTCGACCAAAAAACAACCTGAAGAAAGCCACAGATACAGATC CAAGCCAAGTTTCTCAGCCTAAGAAAACGTTGTCCCCAACAAATGCAGTGGAACCAGGTCAGGTTGCAGA TGCAAAGCAAGGCCCTCCGGAACTTCAGCAATCCAAAGTCCAGGTGCTGGAGGAGAAGGTCGTCCGACTC ACCAGGATGGTGCTGGATCTCCAGTCCACCGTGGTAGGCCTGAAGGAGAACCTCAAACACACCATTCAAG ATGATGGCAGAAAAGAGCCAGACTCCTGGCTCGGTCCCCTGCACCCACAGCCAACGCCTGACAGCCCCCT TGCGGGGGACGCAGAGCCGAGCCAGCTTCCCGGAATCCCCAGCAGCAAGGAATCTGGTATGAAGGACATC AAGTCTGAGTTGGCTGAGGTCAAAGACACACTGAAGACCAAGAGTGACAAGCTAGAAGAGCTGGACGGGA AGGTGAAGGGCTATGAAGGACAGCTCAAGCAGCTGCAGGAGGCGGCTCAGGGTCCCACGGTCACCATGAC AACCAATGAGCTGTACCAAGCCTATGTGGATAGCAAGATCGATGCCCTGAGGGAGGAGCTCATGGAAGGC ATGGACCGGAAGCTGGCTGACCTGAAGAATACGTGCGAGTACAAGCTGGTGGGGCTCCAGCAGCAGTGTG ATGACTACGGCAGCAGCTACCTCGGAGTGATAGAGCTGATCGGGGAGAAGGAGGCGAGCCTGAAAAAAGA CATTGCTGACCTGCGAGCCCAGCTGCAGGATCCTGTAGCCCAACCAAGCTGTTGCAATGGTCAAAAGAGC AGCGATTTCGGCCCACAGATCAAGGCGCTAGACCAGAAAATCGAGAGAGTTGCCGAGGCCACCAGGATGC TGAATGGACGGCTGGACAATGAGTTCGACCGCCTCTCCGTTCCAGAGCCAGACGCAGACTTTGATGCGAG ATGGACCGAGCTCGACGCAAGGATCAATGTGACGGAGAAGAACGCAGAGGAGCATTGCTTTTACATTGAG GAGACCCTGCGGGGGACCATCAACGGCGAAGTGGATGACTTGAGGAAGCTTCTGAATGAGAAGATCCACT CCCTGGAAGACCGTCTGGGAATTGTTCTGCAAGCGGCTAACAGCTCAGACGTGGAGTTGACACCCATGGG CCCAGCCTTGCCAGAGCAGCCAGGGGCAGAGAATGAGCAGGTCCTAATGGAGTTGAGCCGCCTGAAGGAC AAAGTTCAAGTGGTTGAGGACTTCTGCCTGCAGAGCTTGCCCCACGGGATAGATGGAGCTTTGCCTAGTG TAGAGGACCTCACGCATGTCAGCCTGAGCCTTTTAGAATCCCTCAATGATACGATGCACAGGCAGTTCCA AGAAACGAGTCACAGCATCCAGAAACTCCAAGAGGACGTCAATGCCCTCCATTCTCAACTGAATCACTCC GAATGTACGGGGACTTACTTGCAGAACGGAGTGAGCGACAGCCGAACAGGTGACAGCATGGAAGCAAGTG GGTTTACTAAGACAGGGGAGCAAGAGAGGACAGTGGGGACTGTCCCGTCTCCTGGGACCCCCGCGGCTCC ATGCTGTGGTCAGCTGGAGGAGCGCTGGCAGAAGCTGCAGAACCAAATGTTGGCTGAGCTAGACACGTGT AAGGAGAGTGCTCACGGGGTTCAGAGTGGGGTGTCTGCGATCGAGGGCAGGGTGTTCCAGCTGGAGCAGA CATGCAGAAGGCTGGATACCATCTCAGGGAGTCTGCAGAGGATCAAGGAGGGGCTCGGTAAGCATGTGGG CAGCTTGTGGAACTGCATCCGGCAGATGAACGGGACGCTCAAATCGCACTCCAGAGACATCTCTGGCCTC AAGAATTCTGTGCAGCAATTCTATAGCCATGTTTTCCAGATTTCTACCGACTTGCAAGATCTGGTCAAGT TTCAGCCATCAGCAACAGAGGAACCCTCAGAAGCAACCGAGGGACCGTCTGGGAAGACTCCCCTAGAATC CACCAGACCTTCAGAGGAGGCCCCCACTGAACCACCCCGGCTGACCCCACTGCCAGAAGACCCTGCAGGA CCACCACAGACAGGCCAGCAGCCAGTATTGCCCCAGAGGCCCCTGCAGCCACCACCTTTGCCTGCCTGGC CTGGTCGCACGGGGTTGCCCTTCTTGCCTGGCTCTAGTGGCGTCATCATGGAGACTGGAGAGGCGGGGCC TCCAGGCAGGATGGGCGTGTCCGGAAGGGGCCTGCCTCGGGGAGTGGATGGCCAGATGGGGCAGGGGCCC ATTCATAGCTCGGAAGGCTATGCAGGGGCACCAGGCTACCCCAAGTCACCTCCTGTAACCACCCCAGGGG TGCCGCTGCCCACTCTGGTGTCCTTTTCAGCCGGTCTTACCCAGAAGCCTTTTCCCAGCGATGGAGGCGT TGTTCTCTTCAATAAGGTGCTGGTGAACGATGGGGATGTATACAACCCCAACACTGGGATCTTCACCGCA CCATACGACGGGCGCTACTTGATCACAGCCACCCTCACCCCTGAGAGAGACACCTATGTGGAAGCCGTCC TGTCTGTCTCCAACGCCAGTGTTGCCCAGCTGCACACGGCTGGGTACAGGAGAGAGTTCCTGGAATACCA TCGTCCCCCTGGGGCTGTGCATACCTGTGGGGGCCCGGGGGCCTTCCACCTCATTGTGCACTTGAAGGCA GGAGATGGAGTCAATGTTGTGGTGACTGGGGGCAGACTGGCTCACACAGACTTTGATGAAATGTACTCCA CCTTCAGTGGTGTTTTCTTGTACCCTTTCCTTTCTCACCTCTAGGGAGACCAGGAAGGGAAGAAAGGCTG TGAAAACTCCAAAGCTTTAATATATTTGGTTTATGTAACTGGAGCACTGATCTAGCTTGCTGCACACACT CTGCCTGCCCCCAGGATAAAGGCCTGCCATCCCTCGTTTCCAGACAGCTGTGGAAGTGACACATGGTTAC TTCTGTCACTGGACAACCAGAAGACTCGTTATGTCCCCTTGGCTGGAAGCTGGCTTCTCCTGTGCCGCCT ATAAGCCAGCTCTGCTCGAAGTTCTTGGGTTGAATCTACTTGGTGCTGTTCCGACTGGCCTGGGCCTGAT GCAGCTGGAAGACACTGTAGCGGATGCGCCTCTCTACACTGCACGTAGAAGATTTAAACACTGAAGTGCT TATGTGGCCTGGACCCAGAGAGTTTAACATGGGCTGTTGTTCCACACTTGGTTGCTGTTACCGTCTGGTA CTGATCTCTGTTCCCCTGGGCCCTGCTACCAAGGTGGATGTGAGAGACATTATTTATGAAACTTGCCAGG CTCCTTAAATGACATGTACAATATAAGTGCAAAGACAGGGAGCGTCAATAAAGATGTTGGAATCACTAAA AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6510220 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6510220 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14393 same embryo
	EMAGE:14391 same embryo
	EMAGE:14392 same embryo
	EMAGE:14394 same embryo
	EurExpress:euxassay_004216 same experiment
	MGI:4824543 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source