Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14452

Tnnt3 troponin T3, skeletal, fast ( MGI:109550)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14452 EMAGE:14452 EMAGE:14452 EMAGE:14452 EMAGE:14452
"Pseudo-wholemount" of euxassay_004291. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004291_01 euxassay_004291_02 euxassay_004291_03 euxassay_004291_04
EMAGE:14452 EMAGE:14452 EMAGE:14452 EMAGE:14452 EMAGE:14452
euxassay_004291_05 euxassay_004291_06 euxassay_004291_07 euxassay_004291_08 euxassay_004291_09
EMAGE:14452 EMAGE:14452 EMAGE:14452 EMAGE:14452 EMAGE:14452
euxassay_004291_10 euxassay_004291_11 euxassay_004291_12 euxassay_004291_13 euxassay_004291_14
EMAGE:14452 EMAGE:14452 EMAGE:14452 EMAGE:14452 EMAGE:14452
euxassay_004291_15 euxassay_004291_16 euxassay_004291_17 euxassay_004291_18 euxassay_004291_19
EMAGE:14452 EMAGE:14452 EMAGE:14452 EMAGE:14452 EMAGE:14452
euxassay_004291_20 euxassay_004291_21 euxassay_004291_22 euxassay_004291_23 euxassay_004291_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14452Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14452_wholemount_strong.wlz
14452_wholemount_moderate.wlz
14452_wholemount_weak.wlz
14452_wholemount_possible.wlz
14452_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14452_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
diaphragm
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 moderate expression: see section 21 22 23 24
limb
strong strong
regionalstrong expression: see section 01 02 03 04 05 07 09 10 not examined expression: see section 06
vertebral axis musculature
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 10 11 12 13 14 15 16 17 18 19 20 21 moderate expression: see section 09 22 23 24
anterior abdominal wall musculature
strong strong
regionalstrong expression: see section 09
tongue
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16 17 18 moderate expression: see section 01
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6295
Entity Detected:Tnnt3, troponin T3, skeletal, fast ( MGI:109550)
Sequence:sense strand is shown

>T6295
TTGATGACATCCAGAAGAAGCGTCAAAACAAGGACCTCATGGAGCTCCAAGCCCTCATTGACAGCCACTT
TGAAGCTAGAAAGAAAGAAGAAGAGGAGCTAATCGCACTTAAAGAAAGAATTGAGAAGCGCCGTGCAGAG
AGGGCTGAGCAGCAAAGAATTCGCGCTGAGAAGGAGCGGGAACGCCAGAACAGATTGGCGGAGGAGAAGG
CCAGAAGAGAGGAGGAGGATGCCAAGAGGAGAGCTGAAGATGACATGAAGAAGAAAAAGGCTCTGTCCTC
CATGGGTGCCAACTACAGCAGCTACCTGGCCAAGGCTGACCAGAAGAGAGGCAAGAAACAGACAGCCCGG
GAAATGAAAAAGAAGATTCTTGCCGAAAGGCGCAAGCCTCTGAACATTGACCATCTTAGCGATGACAAGC
TGAGGGACAAGGCCAAGGAACTCTGGGATACCTTGTACCAACTGGAGACTGACAAATTCGAGTTTGGGGA
GAAGCTGAAACGTCAGAAATATGATATCAC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GAAACCAAGACCCAAACTTAC; Reverse Primer - name:unspecified, sequence:TTTATTCCTAGACCCCAGAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14451 same embryo
 EMAGE:14450 same embryo
 EMAGE:14454 same embryo
 EMAGE:14449 same embryo
 EMAGE:14453 same embryo
 EurExpress:euxassay_004291 same experiment
 MGI:4828861 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS