EMAGE mouse gene expression spatial database

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EMAGE:14458

Cul4b cullin 4B ( MGI:1919834)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004283. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004283_01	euxassay_004283_02	euxassay_004283_03	euxassay_004283_04

euxassay_004283_05	euxassay_004283_06	euxassay_004283_07	euxassay_004283_08	euxassay_004283_09

euxassay_004283_10	euxassay_004283_11	euxassay_004283_12	euxassay_004283_13	euxassay_004283_14

euxassay_004283_15	euxassay_004283_16	euxassay_004283_17	euxassay_004283_18	euxassay_004283_19

euxassay_004283_20	euxassay_004283_21	euxassay_004283_22	euxassay_004283_23	euxassay_004283_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14458_wholemount_strong.wlz

14458_wholemount_moderate.wlz

14458_wholemount_weak.wlz

14458_wholemount_possible.wlz

14458_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14458_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6125
Entity Detected:	Cul4b, cullin 4B ( MGI:1919834)
Sequence:	sense strand is shown >T6125 GTCGCAGTCGAAGACAGACGTCTTCCAGAAGGTCTCTGATTCACGAGGTATGAGAGCCCCAACGGATGAA TGTTAGAACAAAATCTTTGTTGATCCTCCAGTGACTCCATCAAAGAGAGGATGTCACGTTCAACTAGGTC TAAAGAAAGAAGAGAGAATGACACCGACTCAGAAGATAACAGTTCGGAAACCTCCAATCAAGAGCGCCGA AGGTGTCGCCAAGGTCCGCCCCGCCCTCCCTATCCACCTCTTTTACCCCCCGTGTTCCCTCCCCCAACCC CCCCACCTCAAGTCCGGCGGACCCGGGGGCTTCAGGATCTGGGAGCTATGAAAAGTGTCTGCCCAGGCAC TTCCGGTTTTTCTTCCCCCAATCCCTCTGCTGCTTCTGCTGCTGCTCAGGAGGTCAGATCTGCCACTGAT GGTAATACCAGCACCACTCCGCCCACCTCTGCCAAGAAGAGAAAGTTAAACAGCAGCAGTAGCAGCAGTA ACAGTAGTAACGAGAGAGAAGACTTTGATTCCACCTCTTCCTCTTCCACCCCTCCACAACCCAGAGATTC GGCGTCCCCTTCAACCTCGTCCTTCTGCCTTGGGGTTCCAGTGGCCACTTCCAGCCACGTACCTATACAG AAGAAGCTGCGGTTTGAAGACACCCTGGAGTTTGTAGGGATTGATACGAAAATGGCTGAGGAATCTTCCT CCTCATCTTCCTCATCTTCGCCAACTGCTGCAACCTCACAGCAGCAGCAGCAGCAACAACTTAAAACTAA GAGTATATTAATTTCTTCTGTGGCTTCAGTGCACCATGCAAACGGCCTGGCTAAATCTTCTACCGCGGTC TCTAGCTTTGCTAACAGCAAGCCCGGCTCAGCTAAGAAGTTAGTGATCAAGAACTTTAAAGATAAGCCTA AATTACCAGAAAACTATACGGATGAGACATGGCAAAAGCTAAAAGAAGCAGTGGAAGCCATTCAGAATAG TACTTCAATTAAGTACAATTTAGAAGAACTCTACCAGGCTGTAGAAAATCTTTGTTCTCATAAGATTTCT GCAAATTTGTATAAGCAGCTGAGGCAGATATGTGAAGATCACATCAAAGCACAGATTCATCAGTTCAGAG AGGATTCATTGGATAGTGTTCTTTTTCTCAAAAAGATCGATAGATGCTGGCAAAACCACTGTAGGCAAAT GATAATGATCAGGAGCATTTTTTTGTTTCTGGATAGAACTTATGTTCTTCAGAATTCAATGCTACCCTCC ATTTGGGACATGGGATTAGAGTTATTTAGGGCTCATATTATAAGTGATCAAAAAGTACAGACAAAAACGA TTGATGGCATCCTTCTCTTGATTGAGAGAGAGCGGAATGGTGAAGCAATTGATAGAAGTTTACTTCGAAG CCTTTTAAGTATGCTGTCTGATTTGCAAATTTACCAAGATTCTTTTGAACAGCAATTTTTGCAAGAAACT AATCGGCTTTACGCAGCCGAAGGTCAGAAGTTAATGCAAGAAAGAGAGGTTCCTGAGTATCTTCATCATG TTAATAAACGCCTAGAAGAAGAAGCTGACAGACTTATTACTTACTTAGATCAGACCACCCAGAAGTCACT AATTGCTTCTGTTGAAAAACAACTTCTAGGTGAACACTTAACGGCAATTCTTCAAAAAGGTTTAAATAGC CTCCTTGATGAAAACCGAATTCAAGATTTGTCTCTCCTGTATCAGCTCTTCAGTAGAGTTCGGGGTGGAG TTCAGGTTCTCTTACAGCAGTGGATTGAGTATATCAAGGCTTTTGGAAGTACCATTGTTATTAATCCTGA AAAAGATAAAACTATGGTTCAAGAATTATTGGACTTTAAAGATAAAGTTGACCATATAATTGATACCTGC TTTCTGAAAAATGAAAAATTTATCAATGCCATGAAAGAAGCTTTTGAAACATTCATTAATAAAAGACCAA ATAAGCCAGCTGAACTGATAGCTAAGTATGTGGATTCAAAACTTCGTGCAGGCAACAAAGAAGCTACAGA TGAAGAGCTTGAGAAAATGTTGGATAAAATAATGATTATATTTAGATTTATCTATGGTAAAGATGTTTTT GAGGCCTTCTATAAGAAAGATTTAGCCAAACGCCTATTAGTTGGCAAGAGTGCATCAGTAGATGCTGAAA AATCAATGCTGTCCAAACTGAAACACGAATGTGGAGCCGCTTTTACCAGCAAGCTTGAAGGAATGTTCAA AGACATGGAGCTTTCTAAAGATATCATGATTCAGTTTAAACAGTATATGCAGAACCAGAATGTACCTGGC AATATTGAATTAACTGTTAATATCCTGACAATGGGTTACTGGCCAACATATGTGCCTATGGAAGTCCATT TGCCGCCAGAGATGGTAAAGCTTCAGGAAATTTTCAAGACATTTTACTTAGGCAAGCACAGTGGCAGGAA ACTCCAGTGGCAGTCAACCTTGGGACACTGTGTGCTAAAAGCTGAATTTAAAGAGGGCAAAAAAGAACTT CAGGTCTCTCTTTTTCAAACCATGGTGCTGCTAATGTTTAATGAAGGAGAAGAATTCAGTTTAGAAGAGA TCAAGCATGCTACTGGGATAGAGGATGGAGAATTAAGAAGAACACTGCAGTCACTAGCCTGTGGCAAAGC TAGAGTTCTGGCTAAAAATCCTAAGGGCAAAGATATTGAAGATGGTGACAAATTTATTTGTAATGACGAT TTCAAACACAAACTATTCAGGATAAAGATCAATCAAATCCAGATGAAAGAAACGGTTGAAGAACAAGCAA GCACTACAGAAAGAGTATTTCAAGACAGACAGTACCAAATTGATGCTGCGATCGTTCGAATTATGAAGAT GAGAAAAACACTTAGCCACAATCTTCTTGTTTCAGAAGTCTACAATCAGCTAAAATTTCCAGTTAAACCT GCTGATCTTAAGAAGAGAATAGAATCCCTCATTGACCGGGACTACATGGAAAGAGATAAAGAAAATCCAA ATCAGTACAACTATATTGCATAGAATGTTGGCCTTGGAACTTGTCATAATGCTGTAGCCATTGGATAAAC TAATTTGTTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3494708 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3494708 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14459 same embryo
	EMAGE:14457 same embryo
	EMAGE:14456 same embryo
	EMAGE:14455 same embryo
	EurExpress:euxassay_004283 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source