EMAGE mouse gene expression spatial database

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EMAGE:14483

Sln sarcolipin ( MGI:1913652)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005363. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005363_01	euxassay_005363_02	euxassay_005363_03	euxassay_005363_04

euxassay_005363_05	euxassay_005363_06	euxassay_005363_07	euxassay_005363_08	euxassay_005363_09

euxassay_005363_10	euxassay_005363_11	euxassay_005363_12	euxassay_005363_13	euxassay_005363_14

euxassay_005363_15	euxassay_005363_16	euxassay_005363_17	euxassay_005363_18	euxassay_005363_19

euxassay_005363_20	euxassay_005363_21	euxassay_005363_22	euxassay_005363_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14483_wholemount_strong.wlz

14483_wholemount_moderate.wlz

14483_wholemount_weak.wlz

14483_wholemount_possible.wlz

14483_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14483_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 23

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 23

heart atrium

moderate

regional

moderate expression: see section 08 09 12 13 14 15 16 weak expression: see section 06 07 10 11

tongue

strong

regional

strong expression: see section 12

tongue muscle

strong

regional

strong expression: see section 12 13 14 15 16 17

tail

strong

regional

strong expression: see section 19

tail mesenchyme

strong

regional

strong expression: see section 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8286
Entity Detected:	Sln, sarcolipin ( MGI:1913652)
Sequence:	sense strand is shown >T8286 TGGAGGTGGAGAGACTGAGGTCCTTGGTAGCCTGAGTGTGCCCCTGCTCCTCTTCAGGAAGTGAAGACAA GCCTTGGTGTGCAATCACAAGTCCTTCTGGAGTTCTCATCCAGACATTCTGAAGATGGAGAGGTCTACTC AGGAGCTGTTTATCAACTTCACAGTTGTCCTCATCACCGTTCTCCTTATGTGGCTCCTCGTGAGGTCCTA CCAATACTGAGGGGCCATGCTATACTCCAGGGAGTGACTGCTGTGTGCCCCGAGCTTCCAATGCTCTATT GTCATGAGATGCTGCTTCTGGCTCCTCCAGCATCTCTGACCCACACTCACAATGCCTGACACACCGCTGC ACTAGGTCCTTGGCATGTTTTCTAAAGATGCTGTTCTTGTAGGCTGCCCAAAGCTTGCCAGCCAGTGAGC CTTAGCTTTGTTCCCTAGCAAATGTACTATTCAAGTCACCAGGTTAAAATTAAACTGGATTCTTATGATG CAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1397989 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1397989 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14484 same embryo
	EMAGE:14480 same embryo
	EMAGE:14482 same embryo
	EMAGE:14481 same embryo
	EMAGE:14478 same embryo
	EMAGE:14479 same embryo
	EurExpress:euxassay_005363 same experiment
	MGI:4828310 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source