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EMAGE:14512

Rnaseh2a ribonuclease H2, large subunit ( MGI:1916974)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14512 EMAGE:14512 EMAGE:14512 EMAGE:14512 EMAGE:14512
"Pseudo-wholemount" of euxassay_005379. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005379_01 euxassay_005379_02 euxassay_005379_03 euxassay_005379_04
EMAGE:14512 EMAGE:14512 EMAGE:14512 EMAGE:14512 EMAGE:14512
euxassay_005379_05 euxassay_005379_06 euxassay_005379_07 euxassay_005379_08 euxassay_005379_09
EMAGE:14512 EMAGE:14512 EMAGE:14512 EMAGE:14512 EMAGE:14512
euxassay_005379_10 euxassay_005379_11 euxassay_005379_12 euxassay_005379_13 euxassay_005379_14
EMAGE:14512 EMAGE:14512 EMAGE:14512 EMAGE:14512 EMAGE:14512
euxassay_005379_15 euxassay_005379_16 euxassay_005379_17 euxassay_005379_18 euxassay_005379_19
EMAGE:14512 EMAGE:14512 EMAGE:14512 EMAGE:14512
euxassay_005379_20 euxassay_005379_21 euxassay_005379_22 euxassay_005379_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14512Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14512_wholemount_strong.wlz
14512_wholemount_moderate.wlz
14512_wholemount_weak.wlz
14512_wholemount_possible.wlz
14512_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14512_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8315
Entity Detected:Rnaseh2a, ribonuclease H2, large subunit ( MGI:1916974)
Sequence:sense strand is shown

>T8315
GTCAGCATCAAGAGCCGCTGCAGTTTTCGCGGAAAACGCGCGCTGGGACCTGCGCTTGCAGTGTTGTTTT
CTACAATTGTTGGCTGTCGCAGAGGCAGCATGGATCTCAGCGAGCTGGAGAGGGACAATACGGGTCGTTG
TCGTCTGAGTTCTCCTGTACCTGCTGTGTGTCTCAAGGAGCCGTGCGTTCTGGGCGTGGATGAAGCGGGC
CGAGGCCCTGTGCTTGGTCCCATGGTCTACGCCATCTGTTACTGCCCCCTGTCTCGCTTGGCAGATCTGG
AGGCTCTGAAAGTGGCAGACTCTAAGACCTTGACAGAGAACGAGCGGGAGAGGCTCTTTGCGAAAATGGA
GGAGGATGGAGACTTTGTGGGTTGGGCTTTGGACGTCCTGTCTCCAAACCTGATCTCTACCAGCATGCTT
GGGCGAGTCAAGTACAACCTCAACTCCCTGTCACACGATACAGCAGCGGGGCTGATACAGTACGCACTGG
ACCAGAATGTGAATGTCACTCAGGTATTTGTGGACACTGTAGGAATGCCAGAGACATACCAGGCTCGATT
ACAACAGCACTTTCCCGGGATAGAGGTGACAGTCAAGGCCAAAGCTGACTCCCTGTTCCCTGTGGTCAGT
GCTGCCAGCATCTTTGCCAAGGTGGCCCGAGACAAGGCTGTGAAGAACTGGCAGTTTGTGGAAAATTTAC
AGGATCTGGACTCCGATTATGGCTCAGGCTATCCCAATGATCCCAAGACCAAAGCCTGGCTGAGGAAACA
TGTGGACCCTGTGTTTGGCTTCCCCCAGTTTGTACGGTTCAGTTGGAGCACAGCCCAGGCCATCCTGGAG
AAGGAGGCAGAAGATGTCATTTGGGAGGACTCAGAAGCTGAGGAGGATCCTGAAAGACCGGGAAAAATCA
CCTCCTACTTCAGCCAGGGCCCGCAGACCTGCCGCCCTCAGGCCCCCCACAGATACTTCCAGGAGCGAGG
CCTGGAGGCAGCCAGCAGCCTCTAGGAGCCCACATGTGCACCACCTGCCCTTCTAACCCAGGAATAAAAG
CTGTTCAAGAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:1493985 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1493985 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14515 same embryo
 EMAGE:14513 same embryo
 EMAGE:14514 same embryo
 EMAGE:14511 same embryo
 EMAGE:14516 same embryo
 EurExpress:euxassay_005379 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS