EMAGE mouse gene expression spatial database

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EMAGE:14514

Aplp1 amyloid beta (A4) precursor-like protein 1 ( MGI:88046)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005371. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005371_01	euxassay_005371_02	euxassay_005371_03	euxassay_005371_04

euxassay_005371_05	euxassay_005371_06	euxassay_005371_07	euxassay_005371_08	euxassay_005371_09

euxassay_005371_10	euxassay_005371_11	euxassay_005371_12	euxassay_005371_13	euxassay_005371_14

euxassay_005371_15	euxassay_005371_16	euxassay_005371_17	euxassay_005371_18	euxassay_005371_19

euxassay_005371_20	euxassay_005371_21	euxassay_005371_22	euxassay_005371_23	euxassay_005371_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14514_wholemount_strong.wlz

14514_wholemount_moderate.wlz

14514_wholemount_weak.wlz

14514_wholemount_possible.wlz

14514_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14514_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 24 moderate expression: see section 21 22 23

adenohypophysis

moderate

regional

moderate expression: see section 10 11 12 13 14 15

facial vii ganglion

strong

regional

strong expression: see section 04 05 07 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 16 17

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20

vagus x ganglion

strong

regional

strong expression: see section 08 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 17 18

trigeminal v nerve

strong

regional

strong expression: see section 10 17

spinal cord

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 09 14

cervical ganglion

moderate

regional

moderate expression: see section 08 09 16

thoracic ganglion

moderate

regional

moderate expression: see section 09 10 11 12

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 09 11 12 13 14 15

not examined

regional

not examined expression: see section 02 03 04 05 24

neural retina

strong

regional

strong expression: see section 02 03 04 05 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19 20 moderate expression: see section 10

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 14 15 17 18

not examined

regional

not examined expression: see section 15

male reproductive system

strong

regional

strong expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4135
Entity Detected:	Aplp1, amyloid beta (A4) precursor-like protein 1 ( MGI:88046)
Sequence:	sense strand is shown >T4135 GGAGGCGGCGGTGGCGCTGGGAGCTCCTGTCACCGCTGGGGCCGGGTAGGGCCGGGCGGGAGCGCAGGGG ACGTGAGGGCCCGAGGCCCGGACATGGGGCCCACCAGCCCCGCCGCTCGCGGTCAGGGTCGCCGCTGGCG ACCGCCGCCGCTGCCGCTGTTGCTGCCACTGTCATTGCTGCTTCTGCGCGCGCAGCTCGCCGTCGGGAAC CTGGCTGTTGGGAGCCCCAGCGCGGCCGAGGCTCCGGGGTCGGCTCAAGTGGCTGGACTATGTGGGCGTC TAACCCTTCACCGGGACCTGCGCACCGGCCGCTGGGAACCAGACCCACAGCGATCACGACGCTGTCTTCT GGACCCGCAGCGCGTGCTGGAGTACTGCAGACAGATGTACCCCGAGCTGCACATAGCACGCGTGGAGCAG GCTGCACAGGCCATCCCGATGGAGCGCTGGTGTGGGGGTACCCGGAGTGGCAGATGCGCCCACCCCCACC ATGAGGTTGTGCCCTTCCATTGCCTGCCTGGCGAATTCGTGAGTGAAGCCCTGCTAGTGCCCGAAGGCTG TCGGTTCTTGCACCAGGAGCGTATGGACCAGTGTGAGAGTTCAACCAGGAGGCATCAGGAGGCTCAGGAG GCCTGCAGCTCTCAGGGCCTCATCCTGCATGGCTCTGGCATGCTTTTGCCCTGTGGCTCTGATCGGTTCC GAGGTGTGGAGTATGTATGCTGTCCACCTCCTGCAACTCCCAACCCATCTGGGATGGCAGCTGGTGACCC CTCTACCCGGTCCTGGCCCCTGGGGGGCAGAGCAGAGGGAGGTGAGGATGAAGAGGAGGTGGAATCTTTC CCTCAGCCAGTAGACGATTACTTCGTAGAGCCCCCTCAGGCTGAAGAAGAAGAGGAAGAGGAGGAAGAAA GGGCCCCACCTCCCAGCTCCCACACCCCTGTCATGGTTAGCAGAGTCACTCCCACCCCAAGGCCTACTGA TGGTGTGGATGTTTACTTTGGCATGCCTGGGGAAATCGGCGAGCATGAGGGTTTCCTGAGGGCCAAGATG GACCTGGAGGAGCGTAGGATGCGCCAGATTAATGAGGTGATGCGTGAATGGGCCATGGCTGACAGCCAAT CTAAGAACCTGCCAAAGGCGGACAGGCAGGCCCTGAATGAGCACTTCCAGTCCATTCTGCAGACCCTGGA AGAACAAGTGTCTGGTGAACGGCAACGCCTGGTGGAGACCCACGCCACCAGAGTCATCGCTCTGATCAAC GACCAGCGCCGAGCAGCCCTGGAAGGTTTCCTGGCAGCCTTACAGGGCGATCCGCCTCAGGCTGAGCGAG TTCTGATGGCCCTGAGGCGCTACCTGCGCGCCGAGCAGAAAGAGCAGAGGCACACTCTGAGGCACTACCA GCACGTGGCCGCAGTGGATCCTGAGAAGGCCCAGCAGATGCGCTTTCAGGTCCAGACCCACCTTCAGGTG ATCGAAGAGCGAATGAATCAGAGCCTGGGGCTGCTCGACCAGAACCCTCACCTGGCTCAGGAGCTGCGGC CACAGATCCAGGAGCTTCTCCTTGCTGAACACTTGGGTCCCAGTGAACTGGACGCCTCTGTGCCCGGGAG CAGCAGTGAGGACAAAGGTAGCCTCCAGCCTCCCGAATCCAAGGACGATCCCCCAGTGACCCTTCCAAAA GGGTCCACAGATCAAGAGTCATCCTCCTCTGGGAGAGAGAAGCTAACTCCACTGGAGCAGTATGAGCAAA AGGTGAATGCATCCGCCCCGAGGGGGTTTCCCTTCCACTCGTCAGATATCCAGCGGGATGAACTGGCTCC TTCCGGGACTGGAGTGTCCCGAGAGGCCTTGTCAGGTCTGCTGATCATGGGAGCTGGAGGAGGCTCCCTC ATTGTCCTATCCTTGCTGCTTCTGCGCAAGAAGAAACCCTATGGGACTATCAGCCATGGAGTGGTGGAGG TGGACCCCATGCTGACCCTGGAGGAGCAGCAGCTCCGGGAACTTCAGAGGCATGGCTATGAGAACCCCAC CTACCGCTTCCTGGAAGAACGACCTTGACCCCTACCCTAGCTGCCTTCAGCTGAGCCCTACTGCCCTTCT TCCGGCCCCCCAAACCCAACTCCCAGCTTCCGGTGGGGGAGGGAGATCTTGACAAATTCATTCTTGTTTC CCCTTCCTAGTTCCAAATTCCACACCCTTAGAAATCCCCAGCTCCTGTCCCACAAAGGACCTCTTCACCT TAATTTATTTTACGTTAATTTATTGCTCCTTAAGGTGACCTGGGTCCCAGGTATGTATGTCACTCCCTGG AATTCACCATCCCACGTTTCTTCACTAACATCCCAATAAAGTCCTCTTTCCCACCCGGCAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4481838 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4481838 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14515 same embryo
	EMAGE:14513 same embryo
	EMAGE:14511 same embryo
	EMAGE:14516 same embryo
	EMAGE:14512 same embryo
	EurExpress:euxassay_005371 same experiment
	MGI:4823179 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source