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EMAGE:14514

Aplp1 amyloid beta (A4) precursor-like protein 1 ( MGI:88046)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14514 EMAGE:14514 EMAGE:14514 EMAGE:14514 EMAGE:14514
"Pseudo-wholemount" of euxassay_005371. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005371_01 euxassay_005371_02 euxassay_005371_03 euxassay_005371_04
EMAGE:14514 EMAGE:14514 EMAGE:14514 EMAGE:14514 EMAGE:14514
euxassay_005371_05 euxassay_005371_06 euxassay_005371_07 euxassay_005371_08 euxassay_005371_09
EMAGE:14514 EMAGE:14514 EMAGE:14514 EMAGE:14514 EMAGE:14514
euxassay_005371_10 euxassay_005371_11 euxassay_005371_12 euxassay_005371_13 euxassay_005371_14
EMAGE:14514 EMAGE:14514 EMAGE:14514 EMAGE:14514 EMAGE:14514
euxassay_005371_15 euxassay_005371_16 euxassay_005371_17 euxassay_005371_18 euxassay_005371_19
EMAGE:14514 EMAGE:14514 EMAGE:14514 EMAGE:14514 EMAGE:14514
euxassay_005371_20 euxassay_005371_21 euxassay_005371_22 euxassay_005371_23 euxassay_005371_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14514Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14514_wholemount_strong.wlz
14514_wholemount_moderate.wlz
14514_wholemount_weak.wlz
14514_wholemount_possible.wlz
14514_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14514_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
brain
strong strong
homogeneousstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 24 moderate expression: see section 21 22 23
adenohypophysis
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15
facial vii ganglion
strong strong
regionalstrong expression: see section 04 05 07 19 20
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 06 07 16 17
trigeminal v ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 17 18 19 20
vagus x ganglion
strong strong
regionalstrong expression: see section 08 16
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 06 07 08 17 18
trigeminal v nerve
strong strong
regionalstrong expression: see section 10 17
spinal cord
strong strong
homogeneousstrong expression: see section 06 07 08 09 10 11 12 13
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 08 09 14
cervical ganglion
moderate moderate
regionalmoderate expression: see section 08 09 16
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 09 10 11 12
dorsal root ganglion
strong strong
regionalstrong expression: see section 05 06 07 08 09 11 12 13 14 15
not examined not examined
regionalnot examined expression: see section 02 03 04 05 24
neural retina
strong strong
regionalstrong expression: see section 02 03 04 05 24
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 11 12 13 14 15 16 17 18 19 20 moderate expression: see section 10
nasal cavity respiratory epithelium
strong strong
regionalstrong expression: see section 14 15 17 18
not examined not examined
regionalnot examined expression: see section 15
male reproductive system
strong strong
regionalstrong expression: see section 10
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4135
Entity Detected:Aplp1, amyloid beta (A4) precursor-like protein 1 ( MGI:88046)
Sequence:sense strand is shown

>T4135
GGAGGCGGCGGTGGCGCTGGGAGCTCCTGTCACCGCTGGGGCCGGGTAGGGCCGGGCGGGAGCGCAGGGG
ACGTGAGGGCCCGAGGCCCGGACATGGGGCCCACCAGCCCCGCCGCTCGCGGTCAGGGTCGCCGCTGGCG
ACCGCCGCCGCTGCCGCTGTTGCTGCCACTGTCATTGCTGCTTCTGCGCGCGCAGCTCGCCGTCGGGAAC
CTGGCTGTTGGGAGCCCCAGCGCGGCCGAGGCTCCGGGGTCGGCTCAAGTGGCTGGACTATGTGGGCGTC
TAACCCTTCACCGGGACCTGCGCACCGGCCGCTGGGAACCAGACCCACAGCGATCACGACGCTGTCTTCT
GGACCCGCAGCGCGTGCTGGAGTACTGCAGACAGATGTACCCCGAGCTGCACATAGCACGCGTGGAGCAG
GCTGCACAGGCCATCCCGATGGAGCGCTGGTGTGGGGGTACCCGGAGTGGCAGATGCGCCCACCCCCACC
ATGAGGTTGTGCCCTTCCATTGCCTGCCTGGCGAATTCGTGAGTGAAGCCCTGCTAGTGCCCGAAGGCTG
TCGGTTCTTGCACCAGGAGCGTATGGACCAGTGTGAGAGTTCAACCAGGAGGCATCAGGAGGCTCAGGAG
GCCTGCAGCTCTCAGGGCCTCATCCTGCATGGCTCTGGCATGCTTTTGCCCTGTGGCTCTGATCGGTTCC
GAGGTGTGGAGTATGTATGCTGTCCACCTCCTGCAACTCCCAACCCATCTGGGATGGCAGCTGGTGACCC
CTCTACCCGGTCCTGGCCCCTGGGGGGCAGAGCAGAGGGAGGTGAGGATGAAGAGGAGGTGGAATCTTTC
CCTCAGCCAGTAGACGATTACTTCGTAGAGCCCCCTCAGGCTGAAGAAGAAGAGGAAGAGGAGGAAGAAA
GGGCCCCACCTCCCAGCTCCCACACCCCTGTCATGGTTAGCAGAGTCACTCCCACCCCAAGGCCTACTGA
TGGTGTGGATGTTTACTTTGGCATGCCTGGGGAAATCGGCGAGCATGAGGGTTTCCTGAGGGCCAAGATG
GACCTGGAGGAGCGTAGGATGCGCCAGATTAATGAGGTGATGCGTGAATGGGCCATGGCTGACAGCCAAT
CTAAGAACCTGCCAAAGGCGGACAGGCAGGCCCTGAATGAGCACTTCCAGTCCATTCTGCAGACCCTGGA
AGAACAAGTGTCTGGTGAACGGCAACGCCTGGTGGAGACCCACGCCACCAGAGTCATCGCTCTGATCAAC
GACCAGCGCCGAGCAGCCCTGGAAGGTTTCCTGGCAGCCTTACAGGGCGATCCGCCTCAGGCTGAGCGAG
TTCTGATGGCCCTGAGGCGCTACCTGCGCGCCGAGCAGAAAGAGCAGAGGCACACTCTGAGGCACTACCA
GCACGTGGCCGCAGTGGATCCTGAGAAGGCCCAGCAGATGCGCTTTCAGGTCCAGACCCACCTTCAGGTG
ATCGAAGAGCGAATGAATCAGAGCCTGGGGCTGCTCGACCAGAACCCTCACCTGGCTCAGGAGCTGCGGC
CACAGATCCAGGAGCTTCTCCTTGCTGAACACTTGGGTCCCAGTGAACTGGACGCCTCTGTGCCCGGGAG
CAGCAGTGAGGACAAAGGTAGCCTCCAGCCTCCCGAATCCAAGGACGATCCCCCAGTGACCCTTCCAAAA
GGGTCCACAGATCAAGAGTCATCCTCCTCTGGGAGAGAGAAGCTAACTCCACTGGAGCAGTATGAGCAAA
AGGTGAATGCATCCGCCCCGAGGGGGTTTCCCTTCCACTCGTCAGATATCCAGCGGGATGAACTGGCTCC
TTCCGGGACTGGAGTGTCCCGAGAGGCCTTGTCAGGTCTGCTGATCATGGGAGCTGGAGGAGGCTCCCTC
ATTGTCCTATCCTTGCTGCTTCTGCGCAAGAAGAAACCCTATGGGACTATCAGCCATGGAGTGGTGGAGG
TGGACCCCATGCTGACCCTGGAGGAGCAGCAGCTCCGGGAACTTCAGAGGCATGGCTATGAGAACCCCAC
CTACCGCTTCCTGGAAGAACGACCTTGACCCCTACCCTAGCTGCCTTCAGCTGAGCCCTACTGCCCTTCT
TCCGGCCCCCCAAACCCAACTCCCAGCTTCCGGTGGGGGAGGGAGATCTTGACAAATTCATTCTTGTTTC
CCCTTCCTAGTTCCAAATTCCACACCCTTAGAAATCCCCAGCTCCTGTCCCACAAAGGACCTCTTCACCT
TAATTTATTTTACGTTAATTTATTGCTCCTTAAGGTGACCTGGGTCCCAGGTATGTATGTCACTCCCTGG
AATTCACCATCCCACGTTTCTTCACTAACATCCCAATAAAGTCCTCTTTCCCACCCGGCAAAAAAAAAAA
AAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4481838 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4481838 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14515 same embryo
 EMAGE:14513 same embryo
 EMAGE:14511 same embryo
 EMAGE:14516 same embryo
 EMAGE:14512 same embryo
 EurExpress:euxassay_005371 same experiment
 MGI:4823179 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS