EMAGE mouse gene expression spatial database

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EMAGE:14515

1500015O10Rik RIKEN cDNA 1500015O10 gene ( MGI:1926146)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005368. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005368_01	euxassay_005368_02	euxassay_005368_03	euxassay_005368_04

euxassay_005368_05	euxassay_005368_06	euxassay_005368_07	euxassay_005368_08	euxassay_005368_09

euxassay_005368_10	euxassay_005368_11	euxassay_005368_12	euxassay_005368_13	euxassay_005368_14

euxassay_005368_15	euxassay_005368_16	euxassay_005368_17	euxassay_005368_18	euxassay_005368_19

euxassay_005368_20	euxassay_005368_21	euxassay_005368_22	euxassay_005368_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14515_wholemount_strong.wlz

14515_wholemount_moderate.wlz

14515_wholemount_weak.wlz

14515_wholemount_possible.wlz

14515_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14515_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 14 15 16 17 18 19

humerus

moderate

regional

moderate expression: see section 02 03 04 05 17 18 19 20

lateral ventricle choroid plexus

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 moderate expression: see section 18

4th ventricle

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 moderate expression: see section 17 18 19

midbrain ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 moderate expression: see section 13 14

trachea

strong

regional

strong expression: see section 11 12 13

cranium

strong

regional

strong expression: see section 01 02 03 04 05 moderate expression: see section 06 07 15 16 17 18 19 23

clavicle

moderate

regional

moderate expression: see section 07 08 09 10 15 16

scapula

moderate

regional

moderate expression: see section 03 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6112
Entity Detected:	1500015O10Rik, RIKEN cDNA 1500015O10 gene ( MGI:1926146)
Sequence:	sense strand is shown >T6112 CCCACGCGTCCGCGGACGCGTGGGTTGCAGCAGACCCGCAGGGCAGTGTGCCTCGGTGGCATTGAACTGA AGCTTGGCTCTCGGCCTGGCCTGCTGGCTAGTTGCCCACCCTGTGGGTCCCGCCCAGAGCAAGGATACTG GAGCTTTCGCCTGCCTCCCTGAGCCTGGGTCTCCACTCCAGTCATCCCTCCAGCTACTTTGCAGCACTCT GTCGCCATGAGCACCTCGTCTGCGCGGCCTGCAGTCCTGGCCCTTGCCGGGCTGGCTCTGCTCCTTCTGC TGTGCCTGGGTCCAGATGGCATAAGTGGAAACAAACTCAAGAAGATGCTCCAGAAACGAGAAGGACCTGT CCCGTCAAAGACTAATGTAGCTGTAGCCGAGAACACAGCAAAGGAATTCCTAGGTGGCCTGAAGCGTGCC AAACGACAGCTGTGGGACCGTACGCGGCCTGAGGTACAGCAGTGGTACCAGCAGTTCCTCTACATGGGCT TTGATGAGGCTAAATTTGAAGATGATGTCAACTATTGGCTAAACAGAAATCGAAACGGCCATGACTACTA TGGTGACTACTACCAGCGTCATTATGATGAAGATGCGGCCATTGGTCCCCACAGCCGGGAAAGCTTCAGG CATGGAGCCAGTGTCAACTATGATGACTATTAAGCTTCCTGAGGTGCCCACAGAGCTTGTGCCTGCTTCA GTAGGCCTTCTCTACCTATACCACGTGACCATCAGGCTAAAGGAAAGAATATAAGTGCTTTTTGCATTTC ATGCATGTGCTTAACGATATGTCTCACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3493553 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3493553 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14513 same embryo
	EMAGE:14514 same embryo
	EMAGE:14511 same embryo
	EMAGE:14516 same embryo
	EMAGE:14512 same embryo
	EurExpress:euxassay_005368 same experiment
	MGI:4822608 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source