EMAGE mouse gene expression spatial database

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EMAGE:14558

Ano6 anoctamin 6 ( MGI:2145890)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005440. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005440_01	euxassay_005440_02	euxassay_005440_03	euxassay_005440_04

euxassay_005440_05	euxassay_005440_06	euxassay_005440_07	euxassay_005440_08	euxassay_005440_09

euxassay_005440_10	euxassay_005440_11	euxassay_005440_12	euxassay_005440_13	euxassay_005440_14

euxassay_005440_15	euxassay_005440_16	euxassay_005440_17	euxassay_005440_18	euxassay_005440_19

euxassay_005440_20	euxassay_005440_21	euxassay_005440_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14558_wholemount_strong.wlz

14558_wholemount_moderate.wlz

14558_wholemount_weak.wlz

14558_wholemount_possible.wlz

14558_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14558_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8805
Entity Detected:	Ano6, anoctamin 6 ( MGI:2145890)
Sequence:	sense strand is shown >T8805 GCAGGCTGAGCAGCGCCTGCTGAAGCAGAGCGCGGGCGGAGCGCAGCGGGGCAGTGCCGAGAGGGCAGAG CGGAGCTTGCGGCCCGAGCCGAGCGGAGCCGAGCGGAGCCGAGAGCAGCCGAGTGACGGTCTCCGGGAGA CATGCAGATGATGACTAGGAAGGTCCTGCTGAACATGGAGCTGGAGGAGGACGACGATGAGGATGGAGAC ATTGTGCTGGAAAACTTTGACCAGACAATTGTCTGCCCCACCTTTGGATCACTGGAGAATCAGCAGGACT TCAGGACTCCAGAGTTTGAAGAATTTAACGGGAAGCCCGACTCCCTCTTTTTCACCGATGGCCAGAGGCG AATCGACTTCATCCTCGTGTATGAAGATGAGAGCAAAAAGGAGAACAATAAGAAAGGGACAAATGAGAAA CAGAAGAGGAAAAGACAAGCATACGAATCTAACCTTATCTGCCATGGGCTGCAGCTGGAAGCAACAAGAT CTGTTTCTGATGACAAGCTTGTGTTCGTAAAAGTGCACGCGCCCTGGGAAGTGCTGTGCACCTATGCTGA GATCATGCACATCAAACTCCCGCTAAAGCCAAACGACCTGAAAACGCGCTCGCCCTTTGGCAACCTCAAC TGGTTCACCAAGGTCCTCCGGGTGAACGAGAGTGTCATCAAGCCAGAGCAGGAGTTCTTCACTGCCCCTT TTGAGAAGAGCCGGATGAATGATTTCTACATCCTCGATAGAGATTCCTTCTTCAACCCTGCCACCAGAAG CCGCATTGTTTATTTCATCCTCTCTCGGGTCAAATACCAAGTGATGAACAACGTTAACAAATTTGGGATT AATAGACTGGTCAGCTCTGGAATCTACAAAGCAGCGTTTCCTCTGCACGACTGCAGATTCAACTATGAGT CGGAGGACATCAGTTGTCCTAGCGAGCGTTACCTCCTGTACAGAGAATGGGCTCACCCTCGGAGTATATA CAAGAAGCAGCCCTTGGATCTTATCAGGAAGTATTACGGCGAGAAGATTGGAATCTACTTTGCTTGGCTG GGCTATTACACGCAGATGCTCCTTCTCGCAGCTGTGGTGGGCGTGGCCTGCTTCCTCTATGGATATCTTG ATCAGGATAACTGCACTTGGAGCAAAGAGGTCTGTGATCCTGACATCGGTGGCCAGATCCTGATGTGCCC CCAGTGTGACAGGCTGTGTCCGTTCTGGAGGCTGAATATCACCTGTGAATCTTCTAAGAAATTGTGCATC TTTGACAGTTTTGGAACCCTGATCTTCGCTGTATTTATGGGAGTGTGGGTTACATTGTTTTTGGAGTTTT GGAAGCGGCGCCAGGCAGAGCTCGAATATGAATGGGACACCGTTGAGCTACAGCAAGAGGAACAGGCCCG GCCAGAGTATGAGGCCCAGTGCAATCACGTGGTGATAAACGAGATCACTCAGGAGGAGGAGCGCATCCCA TTTACCACCTGTGGGAAGTGCATCCGGGTCACGCTGTGTGCGAGCGCCGTCTTCTTCTGGATCCTGCTCA TCATCGCCTCTGTGATCGGGATCATTGTCTACAGGCTGTCCGTGTTCATCGTATTTTCCACAACGCTCCC CAAGAACCCCAACGGGACAGACCCGATCCAGAAGTACCTGACCCCACAGATGGCCACATCCATCACAGCC TCCATCATCAGCTTCATCATCATCATGATCCTCAACACGATCTACGAGAAGGTGGCCATCATGATCACCA ACTTCGAGCTCCCAAGGACCCAGACGGATTATGAGAACAGCCTGACCATGAAGATGTTCTTGTTCCAGTT TGTCAACTATTACTCCTCATGCTTCTACATCGCATTCTTCAAGGGCAAATTCGTGGGCTATCCCGGGGAC CCAGTGTACTTGCTGGGCAAATACAGAAGCGAAGAGTGTGACCCGGGGGGCTGCCTCCTTGAACTGACCA CACAGCTGACGATCATCATGGGGGGAAAGGCAATCTGGAACAACATACAAGAAGTCTTGCTCCCATGGGT TATGAATCTAATTGGACGATATAAAAGAGTCTCGGGATCAGAAAAGATAACCCCACGATGGGAACAGGAT TACCATCTGCAGCCCATGGGCAAGCTGGGATTGTTCTATGAGTATCTTGAAATGATTATTCAGTTTGGGT TCGTCACTTTATTTGTGGCCTCTTTCCCACTGGCTCCCCTGCTGGCTCTGGTGAACAATATTTTGGAAAT AAGAGTGGACGCGTGGAAGCTCACAACCCAGTTTAGGCGCATGGTGCCCGAAAAAGCCCAGGATATCGGC GCGTGGCAGCCCATCATGCAAGGAATAGCCATCCTGGCCGTGGTGACCAACGCCATGATCATTGCTTTCA CATCAGACATGATCCCCCGCCTGGTGTATTATTGGTCTTTCTCTATCCCTCCCTACGGGGACCACACTTA TTACACCATGGACGGCTACATCAACAACACTCTCTCTGTCTTCAACATCACGGACTTCAAGAACACAGAC AAAGAAAACCCATACATTGGGCTTGGTAACTATACCTTGTGCAGGTACCGTGACTTCCGAAACCCACCTG GGCACCCACAAGAGTATAAACACAACATCTACTATTGGCACGTGATTGCCGCCAAGCTGGCCTTCATCAT TGTCATGGAGCACATCATCTACTCTGTGAAGTTCTTCATTTCCTACGCAATTCCAGATGTGTCCAAAATC ACGAAAAGCAAGATCAAGAGGGAGAAATACCTCACGCAGAAGTTGCTTCACGAGAGTCACCTCAAAGACC TGACCAAAAACATGGGGATCATCGCCGAGAGGATAGGAGGAACGGTTGACAACAGCGTGCGGCCAAAACT CGAATGAGCGGGAGCTTTAGGCCTGGAGAAGCACTTTAAAGAGAATTAGCTGTCAGACGTGTGTGAAGCA CTAGAGAGGATGGCATTGGAACTCCTAGGCAGGCGGAGTCTTGCCCATGCCCATTTCTGCCTGTGTCATC TTCCAGTTTCAGCAAGCAGTGTGGAAACTGGAGAACAGAAAACTGATCAGGCAGAGCCTAAAACCACCTA CCTGGAGGTCCTGAACATGCTCCCTCTTTTGATAAATTGGAATTTTAGTAACAGGAAAAAAAGCCCTGAG GTGTGCCCAAGTTCTCCCCGGGACTTCGCTTTTCTGACCTCACGTGTGCAGAGCTCCTTTTCTGTGCTTA ATTTTGCATTTTGCCAAATCTATCCCAGACCTATTTTGTCCGGACAGCACCATCACCAAAGACCAGGCAG ACCTCAGCCTTAATCGCCTCTGTGTTATCAACAATAGCCAGCTCAGGATTGAGTTTGGATGCTGTGTTAG GTCCCAGGTAGGCTCAAGAGTTCACAGTATTTCAGTGTCTTACTGTACACTCCAAGGAAGGAGACTCCGC TGCGTATGAAACAGCTTCTGTATCATTTCAGCCTGTATTTCCACCTCCAAGAAATACTCGTCTTAGATTG GCATGAAGATGGAGGGTATTGAATGAAGAAGCCTGTCCCTGTTACTCCACGGTGACATTTGCTCTCTAGC AATACTCCCAGCATGCTCTGGGTCTGACCAATGTTGACTGGCTTTCAGATCTTAAGGGTATATAAGATGA AAAGGTAGCAGCCCTTGTTGATCTGAACGGTTAATTTATCTCTTCTTGGTAGATGATAATCAAATGATTG TAACTGTCTCGTCCTCAGCATCATGGGCCGGGAAGCCCATCCACTTCATGAGAACGGACTTAGCAGATGC AGAAGGGTGGCACTACCAATCACCAAGCGCCTTCTGCCTTGCTCGCTGAAAAAGGCTGGTGGCCTGACGA CACTGGGCGTAGTCAGAGCAACAGCCAGCTACCCAATCACTAGCCATATCCTGTGATCCCTCTGTGGCAG ATGTCACTCACTGGGAACCCTACTGACTTCTCTGCTTGCCACGGTGACCAGCTTCCTTGCCCAGCTCCAT TCGTTGCCTGCTAACTCGTTTGTAATCACAGCTGGGCTGGAGACAAGTCTACCAAGATTGTTGCCCTTTG AGGAAGGAAACCCTGGGTTGAATTTGAGCCCCAAGCCTCCTCTCAAGCCATCAGGGGGTTATGGGAAAGG CTGGTTTATCTGCTGGTGGCTTGCTGCGAAGCCTCAGCTTCCTAGTGCCTTGCCCTCCCTGTTGCTTGGC TGAGCAGTCTCTCTGCAGCCTGTGCTCCGCTTCTCCTCCAGTCCATCTGGAGGAGACATGGCGATACCTC GCACCCTTGACTGATCACAGAGGTCAGTGCTGGGTGAAGAAGGGAGGACCTGCCTCACACTGTTTGGGGA TCGGGCCGCTGCTGACTGGATGGGTGTGGAATGCCTCGGTGCCTAGGAACCAAGCCAAGGAAAGTGAGCG GTTTTAGAGACTGCCTTCTCAACACCCGTGGTATTAAATGCCCACCACGAATGATTTACTAGAGAATTGA GAATCAACATGATTAATCAAAGCAGTGTTCTGGGCTTTTGAGTAGCCCAGGGGGTCAGTGTGGGAGTATT TCCCCTCCTCCCTTGTCCTGGGAGTCCAGAGCCCGCCATACAGTGTCTATCTGTGCTGAGATAAAAATGA CTACCTTCCTCCCTGAGGGATGCTCTCATTGCACAGCTGAAACCAAGTGCCACTCCTTGCCCCTACCCCC AGATTGCCCTGGAGTGCCCTGCTCCCTCTGTTACCTCTTTTCCAGAGCCTGTGCTCACTTCCTAAGGACA GGAAAGTACTCCCACTCCATACCATAGAAAGAGGAAATTTTCCCTTCCTTTCTTTGTAGACACAAAGAAA AATGTCAAATAAAAAGTGCATTTCTTCATCTATATTCCCCAGCCAAGAACTCCCTTCACAAAGTAGAAAG TACCACTATGGACAGAAGAAGGAGGAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6409332 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6409332 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14557 same embryo
	EMAGE:14562 same embryo
	EMAGE:14561 same embryo
	EMAGE:14560 same embryo
	EMAGE:14559 same embryo
	EurExpress:euxassay_005440 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source