EMAGE mouse gene expression spatial database

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EMAGE:14600

Cdc42ep1 CDC42 effector protein (Rho GTPase binding) 1 ( MGI:1929763)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005560. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005560_01	euxassay_005560_02	euxassay_005560_03	euxassay_005560_04

euxassay_005560_05	euxassay_005560_06	euxassay_005560_07	euxassay_005560_08	euxassay_005560_09

euxassay_005560_10	euxassay_005560_11	euxassay_005560_12	euxassay_005560_13	euxassay_005560_14

euxassay_005560_15	euxassay_005560_16	euxassay_005560_17	euxassay_005560_18	euxassay_005560_19

euxassay_005560_20	euxassay_005560_21	euxassay_005560_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14600_wholemount_strong.wlz

14600_wholemount_moderate.wlz

14600_wholemount_weak.wlz

14600_wholemount_possible.wlz

14600_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14600_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5531
Entity Detected:	Cdc42ep1, CDC42 effector protein (Rho GTPase binding) 1 ( MGI:1929763)
Sequence:	sense strand is shown >T5531 CGACTCTCGGGTAGCGCTGGGACGCGCCGCCGCCGCGCCCGCCCCTGGACCCCCCGGGCTCGACGACACG AGGTCTCCACTCCAGGGTAGGGATCCTTTTAAGGCCCTTCTCCTGCCACCTTTGATCAGCCTCACTAGGA CCAGAAGCAGAGAGAGAACCCACAGACTCCCTCAGCCTCAGCACTGGGGACAGCGCCTTAGTAAAGTCAA GGGTCGCCTTGTGCTCCGCCATCTTCATTGAGAGGAAGGGGCTGAGAAGGATCCCTGAGTCCAGCCTGCC ATCTTCCCTGGCCCCAAGGTGGCATGGACTAACAGTTGTAAGCAATCAAAGCTGATGCCCGGTCCCCAGG GGGGCACAGGAGCCCCCACCATGAGCCTGGGCAAGCTGTCTCCTGTGGGATGGGTGTCCAGTTCCCATGG AAAGAGACGCCTGACAGCAGACATGATCAGCCCCCCACTTGGGGACTTCCGCCACACCATGCACGTAGGC CGTGGTGGGGATGTCTTCGGAGATACGTCCTTCCTCAGCAACCACGGAGGCCGCTCTGGAAACACCCACC GCTCACCCCGAAGTTTCCTGGCCAGGAAGCTCCAACAGGTTCGCAGAGTCGGGGTGCCACCTCGAAGGAT GGCTTCCCCGGCTGCACCATCACCTGCTCCGCCTCCCATCTCGCCCATCATCAAGAACGCCATCTCCCTG CCTCAGCTCAACCAGGCCACCTATGACAGTCTGGTGATGGGCAAGCTTAGTTTTGACAGCACGCCGGCTA GCTCCACAGATGGCCACTCTGGTTACGGCCTGGAGTCTGGCTTCTGTACCATCTCACGCCTGCCACGCGT GGAAAAGCACAGCAACCGTGACCGTGACCGTGACCCGGACCATTCTCAAGACCGAGAGCAAAGTTCTTTC CCCTCTGAGCCTACCCCTAACCCTGAGCTCCGACGCTCTGACTCTCTCTTGTCCTTCCGTTTTGACCTTG ACCTTGGGCCCTCACTCCTCAGTGAGCTGCTAGGGGTCATGAGCCTTTCAGAAGCTCCAGCTGCTGAAAC TCCAGTCCCTACTGCCAACCCCCCTGCTCCTGCAGCAAACCCTGCCCCTACTGCAAAACCCCCAGCCCAT GCGATAACCACTCTAGATGCTGTGACAAGCCTTCCAGCCTCTGCTGTGACAAGCCTTCCAGCCCCTGCGG CGGCAAGCTCCCCATCCCGTGGACATTTCCCCAATGGGGTAACTTCTGTGTTGGGCCCTGCGGCTGAGGC AAAGCCCAGTCCTGTGGGAGAGGGTCCCCAGGTACCCTCCAACATGACCTTTGACAGGCATGGAGCCAGC TGGGGGGCCAGCCGGGCCAGCTGGGGGGCCAGCCGGGCCAGCCGCCACTACACTGAGATGGATGCCCGGC GGGAGCTGGCAGGGGTGTTACCCCAAGTTCATGGCTCCTGGGAGAGTCTGAATGAAGACTGGAGCACGCC TCCAGCCAGTGTCAGGGCTCCTGTGCCCACCTCGGTGCAGGTCAATGCCTTTGAGTTTGCTGATGCTGAG GAAGATGACGAGGTCAAGGTGTAAGGGACTGGCCATGGGCTCCAGGTCCTGTCAGCCTCGGGGCTCACAG AACAGAGGGGCTGAGTTCATTTGCCTAACAGCTACAAGATGTGGTCAACGGGAGTTGCCCCAGATCCTTC CTGGTCTATGCAAGGCAGGCATGGGTACCCGCTGACCTGACCCAGCTCCCTCCACTGTCCCTAGGCCCAC TAGCCCTTCAGTTATAACCTACGCCACCCCCTGTGGTCTGACAGTATGGTCTTTGTGCCCCAGGACACTT CCCTAGTGGGGTGGGGGTTGGGGCAAGGACCACATAGGGCCATTGTCTGTGAGTGGGACTGGCTGCCCCT ACTCATTGAGAGCTACCGCAGAAGGCCTCCACTGTGTTCTCAGTAGCGCGGTTCCAGGCTCCTCCTCAGT TCCCAGAGAAAGGTCAGGCATGACACAGGAGCAAATCTTCTGTGTACCCCAGACCTGCCTCCCATACCTG ATTGTTAAAGCTTCTCTGTTAAGATCTGCCTGCCCAAGGAGGCAGGCCCGGCCCCTGCTGACCTGTGCTT TCTCCTCCTGAGAGTTTGGAAAGTTGAGGACCCCCTTCTGGTGCCCCCCCTCTAGCTAGGAGAGAAGGGG GGCCAGACGAGCTAATGAGTAACCAGCCTGGAATGTTCAACGATAAGAAGCCCAGGTGGGACCCTTATCT GCAGTTCCTGGAACACCCTGGCCCCAGGTGCCAGGAACCAGTATCTGCCCCATAATCCCATGGGGCTGCT TGGCAGGTGCTCAGATCACCAGGAGGGAGACAGGTCCCCATGCAGAGGGTGGCTCAGGAGCACCTCCCAT CCTCTCTTCTCAACAGTACTATACCCCATGCCTCAGTTTCCTGCCCCCCCCCCCCGTACCAAAGCCGGGG GGTGGGGAGCATTAACATGTGTGGAAAATGCTAACAGTTCTTGCTGTGGAAATAATAAATGTGGATTCTT TTATTTGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6441478 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6441478 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14599 same embryo
	EMAGE:14602 same embryo
	EMAGE:14603 same embryo
	EMAGE:14601 same embryo
	EMAGE:14598 same embryo
	EurExpress:euxassay_005560 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source