EMAGE mouse gene expression spatial database

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EMAGE:14603

Nap1l1 nucleosome assembly protein 1-like 1 ( MGI:1855693)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005511. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005511_01	euxassay_005511_02	euxassay_005511_03	euxassay_005511_04

euxassay_005511_05	euxassay_005511_06	euxassay_005511_07	euxassay_005511_08	euxassay_005511_09

euxassay_005511_10	euxassay_005511_11	euxassay_005511_12	euxassay_005511_13	euxassay_005511_14

euxassay_005511_15	euxassay_005511_16	euxassay_005511_17	euxassay_005511_18	euxassay_005511_19

euxassay_005511_20	euxassay_005511_21	euxassay_005511_22	euxassay_005511_23	euxassay_005511_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14603_wholemount_strong.wlz

14603_wholemount_moderate.wlz

14603_wholemount_weak.wlz

14603_wholemount_possible.wlz

14603_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14603_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nucleus pulposus

strong

regional

strong expression: see section 09 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9604
Entity Detected:	Nap1l1, nucleosome assembly protein 1-like 1 ( MGI:1855693)
Sequence:	sense strand is shown >T9604 GAGACAGCTATATCAGGGTCATTTCAGCAAAATCTTGCTAAGTGTATGCAATGATGTCAGCGTTTGGAGG CTGATTATGGGATGGATCCCTGGTTATGGCAGTCTCTTAGATTTGGGGTTTTATTGATCATCTGGTTTCA CTTCAAATAAATGTTTTTCTTACCTTTAAGCAACTCAGTCTTGTTTTTAACACTGTCCTTTTTCCTTGTA GACATTTGGAGTTCTTACAAGATGGCCGACATTGACAACAAAGAACAGTCTGAACTTGATCAAGATTTGG AAGATGTTGAAGAAGTAGAAGAAGAAGAAACGGGTGAAGAAACAAAAATCAAAGCACGTCAGCTAACTGT TCAGATGATGCAAAATCCTCAGATTCTTGCAGCTCTTCAGGAAAGACTTGATGGTCTGGTAGACACACCA ACAGGATACATTGAAAGCTTGCCTAAGGTAGTCAAAAGACGAGTGAATGCTCTCAAGAATCTTCAGGTTA AATGTGCACAGATAGAAGCCAAATTCTATGAGGAAGTTCATGATCTCGAGAGGAAGTATGCTGTTCTCTA TCAGCCTTTATTTGATAAGCGATTTGAGATCATTAATGCAATCTATGAACCTACAGAAGAAGAATGCGAG TGGAAACCAGATGAGGAGGATGAAGTTTCGGAGGAGCTGAAAGAAAAGGCCAAGATTGAAGATGAGAAAA AGGATGAAGAAAAAGAAGACCCGAAAGGAATTCCTGAATTTTGGTTGACAGTGTTTAAGAATGTTGACTT GCTCAGTGACATGGTTCAGGAACATGATGAACCTATTCTGAAGCACTTGAAAGATATTAAAGTGAAGTTT TCGGATGCTGGCCAGCCTATGAGTTTTGTCTTAGAATTTCACTTTGAACCCAATGACTATTTCACAAATG AAGTGTTAACAAAGACTTACAGGATGCGGTCAGAGCCAGATGATTCTGACCCCTTTTCTTTTGACGGACC AGAGATTATGGGCTGTACAGGGTGCCAGATAGATTGGAAAAAAGGAAAGAATGTTACTTTGAAAACCATT AAGAAGAAGCAGAAACACAAGGGGCGTGGGACAGTTCGTACTGTGACTAAAACAGTTTCCAATGATTCTT TCTTTAATTTTTTTGCTCCTCCTGAAGTTCCTGAGAATGGCGATCTGGATGATGATGCTGAGGCGATACT GGCTGCAGACTTTGAAATTGGTCACTTTTTACGTGAGCGCATAATCCCAAGATCAGTGCTGTACTTCACT GGAGAGGCTATCGAGGACGATGACGATGATTATGATGAAGAAGGTGAAGAAGCTGACGAGGAAGGGGAAG AAGAAGGAGATGAGGAAAACGATCCAGACTATGACCCAAAGAAGGATCAGAACCCAGCCGAGTGCAAGCA GCAGTGAGCAGTGACTGGCCTTGAGGACGGCCGCCCTGTAATAGCCTAAACATGACTCATTTACTTACAG CCTTATGGTTTTGTATTTTCTTGGTAGAATCAGTAAGTTTTTAAGGGAAAGGAGATTGATATTTGAAAGA CGAGTTTGTTCTACCTAGCACCCTAGCTCTAGCTCTGTCAGATACGTTAATGCATACATCCTCTCTAATG CATGTTCATTTATTGCTGCAGTTTGGTTCTTCTGGAGTATTTTCATCATTTAGCTATTGGAATACAATTA TGAAAACCAACTGTTGAACATACTTGGAGTAGCTGTTTCTTTCCTAAAGAACCAAAGTTGTTTTCAGCTA ATAGAACAGGTTGAAGTCCGCCTGCATTAGCTGTGTTTTCCCTCATCTTGTTAGAGGGATGCACAGGGCA CGGTGACATCATTTCCCTCATGTTGTTAGAGGGATGCACAGGGCACGGTGACATCATTTGAGGGAGGAAA ATTGTCGTTAAGAATTTCCAGTAAGATCAAACTTAATAACTGAGATTATGAAAGCATTTTTATGTTTTCG ATGATATAAACAAAAGTCAACTCGTTTATAGCAAGGTGAACAAACCCTAGGTTCTCTTGAAGTTGATAGT ATTTAATGTATTTTGAAAATTAACCTCCATTAATTAAATCTTTTCTTTGTGGTAGGGCCTACCTTCTGCT TTCCTGGAAAAGATGAATATACATCATATGAACCTATTTTGAGTTTTTCTGTTGTTTGTTTGCCTAATTT TGTTTTTATAGCCTAAAATAAAAATATGACACAGTTTTAGCTCTCATGGGATGCTGTCTCGGTTTGTACT GATTGAGGCTATTGCAGAGGGTTAGTTGGAATTCAGGGGTGCCTAGTTTTCTATATTGAACAACGCTGGA GGAGCTAATACACACGTGCTTTAAAAAAAGTGATATGCTCTAGTTTGAGCAGAAAGGTACAGTTGTGCTT GTTAGAACTGAAACCTTTGTTTGGACTTTGTTTATTCTGTCCTCAGGAGGATGAGGAGTGTCGGGAGCCA TGCTGTTTATTTACTGTGCATAACCAAGAATGGGTGGGACTGTTGATTCAACTACCTCAACTCTGTTAAG GCATATTGGGGTATATCTCACAAACTCTCAGTCTTACTTGCAAAATGCTCAAAAAATTGGGCCTCTATAC CATGAGTGTGATATTCAGCATTATATTCACACCAAGCTTTGATGATTAAACTGGAGAATGAAGTTTAAAC ACTTCTATTCTAGATCTGAGCATGTGGCTCCAATTTTAGTGGGAAGGAATGAAAGTAGTGTATGATTTCA AGAGATTGAAGAGTTTTGGGATTTTTGAGTTTTACCCCCCTTCAGATATTTCAAGTGACCACATTATAAA ACACCCTAAGGTGGTAACGAGGCAGGGGAGGATGGCATGAAGTTGCATTAAGTTTCAGACCACTGAGAGC CACATTTAAAATACCTCAGGCTAATTACTGTTCACTTTGGGGCAGGAGTAGGTTTTGCCAGGGTATAGTA ATTGGATTGCACTCCAAGGAAAGTTCCTATTGTTAATAAAATTTACGTAGAGGAGAAACGTTTGTTTGAG GTGGTAGTTCTTTCTGTGAGGTTTTAGTATTCGAACTCTTAGAATGTGAACACATTCGAGAGCTGACTTC TTCCTGAGAGTTTTAACTACTGGAAATCCTTAACCAATTATATTTTCTTTATTTTGAGAATGATTTGGTT GCTTTTTCTTAGATGGTTTCTGCTATTTTCTGTAATCATAGTTCATCTAAACACAGCCTATTTTCGTGAA CATTGAAGTTTGTAACGGAAAGATAGGATCTCATTTTCAGTGAACTTCCAGACTAGCGCACTCTTCCCTT CCCTCTGTAGGAAGTAGCTGTTGCCAAAGCGAAGAGGTAGCTGGCTCTTGTATAATGGGAAAGTGCCGTG TGGAGCTTCAGGGGTCAGTGTTTATTATGACATCTGTCAGTCCCCAAATGGTTCTATTCTCTACAAGTCT GAAATATGTAATCTGAAATCCTTAATAAAATTGGATCTAATTTTATAAAATAAAAAAAAAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:30618066 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30618066 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14599 same embryo
	EMAGE:14602 same embryo
	EMAGE:14600 same embryo
	EMAGE:14601 same embryo
	EMAGE:14598 same embryo
	EurExpress:euxassay_005511 same experiment
	MGI:4826593 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source