EMAGE mouse gene expression spatial database

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EMAGE:14630

Tmod3 tropomodulin 3 ( MGI:1355315)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005515. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005515_01	euxassay_005515_02	euxassay_005515_03	euxassay_005515_04

euxassay_005515_05	euxassay_005515_06	euxassay_005515_07	euxassay_005515_08	euxassay_005515_09

euxassay_005515_10	euxassay_005515_11	euxassay_005515_12	euxassay_005515_13	euxassay_005515_14

euxassay_005515_15	euxassay_005515_16	euxassay_005515_17	euxassay_005515_18	euxassay_005515_19

euxassay_005515_20	euxassay_005515_21	euxassay_005515_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14630_wholemount_strong.wlz

14630_wholemount_moderate.wlz

14630_wholemount_weak.wlz

14630_wholemount_possible.wlz

14630_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14630_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 moderate expression: see section 05 06 15 16 17 18 19 20

mandible

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 16 19 20 21 weak expression: see section 15 17 18

maxilla

moderate

regional

moderate expression: see section 05 06 07 08 09 19 20 weak expression: see section 17 18

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 21 22

clavicle

moderate

regional

moderate expression: see section 07 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9605
Entity Detected:	Tmod3, tropomodulin 3 ( MGI:1355315)
Sequence:	sense strand is shown >T9605 GGAACCAGCAATACAGACAGCGCGGTCGGGTTTAGGGGCAGCTGGCGGGAGGATTCGGCAACTCCCTGAA CTTGAGGCACCGCGGAGTGTGGGCGCCGGGAAGAGCGATTCAAACCTTGCGGACTGTGCAGTGGGTCGGC GGCCAGACAGCTATAACCCGTGGTGGAAGTAGCCAGTGAGTACCCTGCACCATGGCACTGCCGTTCCGGA AGGACTTGGGGGACTACAAGGACCTGGATGAGGACGAGCTCCTTGGCAAGCTGTCCGAATCAGAGCTGAA ACAGCTGGAGACTGTCCTGGATGATCTTGACCCCGAGAATGCCCTTCTGCCTGCAGGGTTTCGGCAGAAG AACCAGACATCAAAGTCTGCCACGGGACCCTTTGATAGAGAACGGCTCCTTTCTTACCTAGAGAAGCAAG CATTGGAGCACAAAGACCGGGACGATTATGTGCCCTACACTGGAGAGAAGAAAGGGAAAATTTTTATCCC CAAACAGAAGCCAGCGCAGACTCTTACAGAAGAAACTATATCCCTCGACCCAGAGTTAGAAGAAGCTTTG ACAAGTGCTTCAGACACAGAGCTGTGCGACCTTGCAGCTATTCTTGGGATGCACAATCTGATTGCAGACA CACCGTTCTGTGATGTGCTGGGAAGTAGTAACGGTGTCAACCAAGAGCGTTTTCCCAATGTGGTTAAAGG GGAAAAGATTCTTCCAGTGTTTGATGAGCCACCAAATCCAACCAACGTTGAAGAGAGTTTAAAGAGAATT AGGGAAAACGATGCCCGTCTTGTTGAAGTTAATTTGAATAATATAAAAAATATCCCAATTCCAACTCTAA AAGATTTTGCAAAGACTTTGGAAGCCAACACGCATGTGAAACATTTCAGTCTCGCGGCCACCAGGAGCAA TGACCCCGTGGCTGTTGCTTTTGCAGATATGCTGAAAGTGAACAAAACTTTGAAGAGCTTAAATATGGAG TCCAACTTCATCACAGGCGCAGGGGTTTTAGCACTGATTGATGCTTTGAGAGATAATGAGACCCTGATGG AGCTCAAGATTGACAATCAGAGACAGCAGTTGGGCACCTCAGTAGAATTAGAAATGGCCAAGATGCTTGA GGAAAATACAAATATCCTTAAATTTGGATATCAGTTCACGCAGCAGGGACCCCGAACCAGGGCAGCCAAC GCGATAACGAAGAACAATGACTTAGTGCGCAAGAGGCGAATTGAAGGAGACCACCAGTAAGCGCAGTGAC CTTTGGAAGCCTGCAGATCACCAGAGGCTCGTGTTGGTGGCAGCAGATGTAAAAATTACCTGGGTAGAAG GGAAAGACTGGAAATCTTTTTTAACTATATGCATTATTTTCCTAGCTTATTATATTTGATTTGTAAAATC AGTACTAGGTAGTATTTTGTATTTGTAGCATTTCTACTGAGACCAGTTTAATTTAGCTTAATTGAATGGT TTATGATGATTTTTAGGTAAAAATGCAGTTGTGGAAAGTTTCAGAAGTCGTGTATGTAAGAATAATTTGA ATGAACATCTTGAGGACCAATATTTTTTAAATCAAAGGGACATTGCTGACTCAATATTAATGTTGCTCCT TATACATTAAGGGTAAGGTGCATGGTGGTTTCCTTTTTTAAAAATCCATGAATTTAGGCAAAAGTATGCT TTTTCCTTATTAGGGAAATAAAATGTCGGAATTTTGAAAGGTGGCTCAGAATCCATTATATCTACATAAG CCAAAGTAGGCCTGCAGGGGACACAACAGCCATCTGTGTGCAGGTTTCTGCACACATCTAATGAGTCTCA GCTGCACGGGGCTGGGTGTGTATGTCTCCTATAGTCCTTAGCTCTGTCCCACTGCATACCTTGTTAAACG TATAGTTATAAGTATTTTTCTCTGCTGTGGGCATTACAGTTTTGTAAAAGCTAATGGATAAAAAGCATCC GAGTAGAGAGGTTCTTTTCCCCATAAAAGGCTCTTGGCCAATACTCAGTTATTGAGAGTAAAACAGAAAA AAGAAAGGCGCTGTTAGATGTAGGAGAGTTCAAACCTGAAATTTGCAAGGTGGCCATCTCACGGATGAGC AGTGTCCGTCAGGAACTGACGTGAGCTAGCTATGGTGGGTCACTCCTACAGTCTGAGCAGCTGAGAAGGT AGGATTGCCAGACATTCAAAGTCAGTCTGAACTACATAGGAACCTCCAGGTTAGCCTGGGCTACATATGT GAGAACCTATGTCAGGAAAAGGAAAGAACAAACTTGTTGTCTGATGAGGAGCGCTATATGTGATTCCATA GGTGCAATGTAGTACTGCCTAAACAGTTAATCCCCTCCACCGTGTTCCAGCCAATCACAAAGACTGCTGT TCTTCAGAAACCTCATTTTAGTAGATGTTTCCTTCATCTTGTTTTTTTTTTAACTGCATATAATAAAAAG TAATTTCTGTATCATAGGTTATCTCTATCACCTTAGCGTTAGTTAGAATGAGGGTTTGATCTGTACAGTG GGGTTGGGTGAGGCTGCCCACCGATGCCCTGCTAGAGGACTTTCGTCTGTATCTATGCTCTGCCCCATGC AGCTCCTACAGGAGTGGCAGAGGCACACACAGCAGACAGTGGAGAGGAAGAAGGCCCAGGGAAGCACGAA CAAGTCGACGGTTGCTTAAACCATGCCAGTGCTGGCCTGTGAAGTATTATACAACTGAAGAAAAGACTTC ATCAGTAACGAGAGTGTGAAGTCATCTGATTGAATAGATATCTTTTAAGAATTATAATGGAGAACAGTGT TTACCTTGATCTTTACAAGTCTGGCACCTCATTTTGGTAGCAGTATTTTTAAATTCCTCTGAGATTTTTA TGACATGGTAATAAATTAGGACTTTGTAATAGTTTTAATTTTAAGCTAGAGATTTTTCTAAACCTTCAAG TTTTATGCATGATTATCTAAGAGAATATTCTTCACATTTTTAAATTGCCCTTTTAAAAAGCCTTAAATTG CCCATTTTGTAAAACTATATATTCATATTTGTCTCAAATTATAATTTTCAGTATGTCTACATGAAATTGT ATACATTAAATTCACCATTAGTAGCTAATATTCATGCCTTGAAAATAGATTTGGTGCTCTACACTTACAG CCAAAACCTACTATAAGTAGGTTTAAAGAATACATAAAATACATTTTCCTACGGTGCCTGTCATTTCAAA GACCAAATAAACATTTTGTGTTAAACATATTAGCAGACTGTTGGTCATTTCAAGGAGGATGTTTGTGATC CTTAAGTGGCACGTTTTCACCAATGAAATGAGCTGTGTCCTTTACAAGGGGTGCAATCCTGAAAAGTTAC ATGTAAATAGAACTTTAAAAATTGTTTTATGGGCTGGTGAGATGGCTTAGAGGGTAAGAGTCCTGACTGC TCTTCTGGAGGTCCTGAGTTCAAATCCCAACAACCACATAGTGGCTCACAACCATCCGTAATGAGACCTG ATGCCCTCTTCTGGTGTGTCTGAAGACAGCTACAGTGTACTTACATATAACAATAAATAAATCTTTTTTA AAAAATGTTTTATAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30645500 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30645500 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14627 same embryo
	EMAGE:14629 same embryo
	EMAGE:14628 same embryo
	EurExpress:euxassay_005515 same experiment
	MGI:4828825 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source