EMAGE mouse gene expression spatial database

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EMAGE:14729

Gpr12 G-protein coupled receptor 12 ( MGI:101909)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005675. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005675_01	euxassay_005675_02	euxassay_005675_03	euxassay_005675_04

euxassay_005675_05	euxassay_005675_06	euxassay_005675_07	euxassay_005675_08	euxassay_005675_09

euxassay_005675_10	euxassay_005675_11	euxassay_005675_12	euxassay_005675_13	euxassay_005675_14

euxassay_005675_15	euxassay_005675_16	euxassay_005675_17	euxassay_005675_18	euxassay_005675_19

euxassay_005675_20	euxassay_005675_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14729_wholemount_strong.wlz

14729_wholemount_moderate.wlz

14729_wholemount_weak.wlz

14729_wholemount_possible.wlz

14729_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14729_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex

moderate

regional

moderate expression: see section 01 05 06 07 13 14 20 21 weak expression: see section 02 03 04 08 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8887
Entity Detected:	Gpr12, G-protein coupled receptor 12 ( MGI:101909)
Sequence:	sense strand is shown >T8887 CGGCATGGGAGATGCAATTAGCCAATGTCGGTTTTCAGCGTTTTGGCAAGTGTGCGAGTGTGCATGTGCC GCCTCGGGAGTCCTGATCCGTGTTTCCCTCAGAGACAAACAGCATTTCGGTTGCAGACTTTAGCTTTTGT TTTTAATTCCTGAAGCTCGTGGCATTTTGACACTGATAGCTGAGCCCAGGGTTGTCTGTCTTTCTCTGTG TGTTTTGCATGATCTTGGATTGGCACCCTACTGTACCCAAACATTAAAAAGCCTGTCTTTCCGTTGAAGA GGACAGGGGTTAAAATGAACGAAGACCCGAAGGTCAATTTAAGCGGGCTGCCTCGGGACTGTATAGATGC CGGTGCTCCAGAGAACATCTCAGCCGCTGTCCCCTCCCAGGGCTCTGTTGCGGAGTCAGAACCCGAGCTC GTTGTCAACCCCTGGGACATTGTCTTGTGCAGCTCAGGAACCCTCATCTGCTGTGAAAATGCCGTTGTGG TCCTTATCATCTTCCACAGCCCCAGCCTGCGAGCCCCCATGTTCCTACTGATAGGCAGCCTGGCTCTTGC AGACCTGCTGGCTGGCCTGGGACTCATCATCAATTTTGTTTTTGCGTACCTGCTTCAGTCAGAAGCCACC AAGCTGGTCACCATCGGACTCATTGTCGCCTCTTTCTCTGCCTCTGTCTGCAGTTTGCTGGCTATTACTG TGGACCGCTACCTCTCGCTATATTACGCCCTGACGTACCACTCCGAGAGGACCGTCACCTTTACCTATGT CATGCTAGTGATGCTCTGGGGAACCTCCATCTGCCTGGGGCTGCTGCCCGTCATGGGCTGGAACTGCTTG AGGGACGAGTCCACCTGCAGCGTGGTCAGACCTCTCACTAAGAACAACGCTGCCATCCTCTCCATCTCCT TCCTCTTCATGTTTGCTCTGATGCTTCAGCTCTACATCCAGATTTGTAAGATTGTGATGAGGCACGCCCA TCAGATAGCCCTGCAGCACCACTTCCTGGCTACATCGCACTATGTGACTACCCGGAAAGGGGTCTCGACC CTGGCTCTCATCCTAGGGACCTTTGCTGCCTGCTGGATGCCTTTCACCCTCTATTCCTTGATCGCCGATT ACACCTACCCTTCGATCTATACCTATGCCACCCTCCTGCCCGCCACCTACAATTCCATCATCAACCCTGT CATTTACGCTTTCAGAAACCAAGAGATCCAGAAAGCCCTCTGCCTCATTTGCTGTGGGTGCATCCCTTCC TCGCTGTCTCAGAGAGCTCGGTCTCCCAGCGATGTGTAGCAGCCTTCTCCTCATAGGACGCTGCCTCTAC CAAGCGCTCCCACCTCCCAGGGCGGCCAGTGATTTCCTTCCTTAAATTCTTTGCACTGGATCTCACAAGC AGAAGCAATGACATCTTTTAGACACGTATTGACAGTGGAAATCATCTTACCAGTGTTTTTTAAAAAAAAA ACAAAACAAAACTCGACTTCTCGGCTCAGCATTCTGTTGTTTGGTTTGGGAGTTAGGATTTGTTTGTTTG TTTGCTTGTTTGTTTGTTTGGAGGGTGTAATGGGACCTCATGTGGCCATGAAATTATACAAAAGTCTCGG GATTTTTTAACCTAGGCTTGAAAATAAATCAAAGTTTTAAAGGAAACTGGAGAAGGAAATACTTTTTCTG AAGGAAATACTTTTTTTTTTTTAATCAAGGTAGATCTTCCATTCTGTATGTATCTAACAGGATAGGAGCT TTGCCATATAACCAAAATAGTTTATATAATTACATTTGGAAGGGCTTGTGTTTATTTCTAGGAATTCAGT AATAAGTGACCAGTAACAGAGGCGCGAACTCCTTTCTTTCCTTTCAGCAGTAGTGACTGCTCTTAAGAAT CACTTTGCAGTTTCTCTGTGTTACAGTTTGGTATGCATGGTTACCTGTGGTAGTCAGATCACTAATTGCA ATATTGCCATGTTAAACCCAGAATTAAAAGAGTCATTTTTTCTTCAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6415225 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6415225 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14731 same embryo
	EMAGE:14727 same embryo
	EMAGE:14728 same embryo
	EMAGE:14730 same embryo
	EurExpress:euxassay_005675 same experiment
	MGI:4825169 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source