EMAGE mouse gene expression spatial database

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EMAGE:14771

Ube4b ubiquitination factor E4B, UFD2 homolog (S. cerevisiae) ( MGI:1927086)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005752. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005752_01	euxassay_005752_02	euxassay_005752_03	euxassay_005752_04

euxassay_005752_05	euxassay_005752_06	euxassay_005752_07	euxassay_005752_08	euxassay_005752_09

euxassay_005752_10	euxassay_005752_11	euxassay_005752_12	euxassay_005752_13	euxassay_005752_14

euxassay_005752_15	euxassay_005752_16	euxassay_005752_17	euxassay_005752_18	euxassay_005752_19

euxassay_005752_20	euxassay_005752_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14771_wholemount_strong.wlz

14771_wholemount_moderate.wlz

14771_wholemount_weak.wlz

14771_wholemount_possible.wlz

14771_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14771_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8747
Entity Detected:	Ube4b, ubiquitination factor E4B, UFD2 homolog (S. cerevisiae) ( MGI:1927086)
Sequence:	sense strand is shown >T8747 GTTTTGATGAATAATTCTTGGTGGGGCGAGGGGGTAAGAGTAGGGTTGGAGGGGTAGGAGGATTTACGCT TCCAGCGAGGGCTTCGCCAGTCCCGTCCCTCCCCTGCCCCCCACCGGGGTTCTCGCGTGGAGGCGGGGCG AGCCTGGCCTGCTTTTAGAGGGGAGGGGCTTCCCGGTCCGGTGCTGGGCTCTGCCTGGGCTGCTGTAGGG TCGCCGCGCCTCGGGGGCATCCAGTGTGCAGTGGAGCTGTTCCCGCCTGGGCCCAGGGGTGGGACCCGGA GCAGGGTCTGCGGGAAAAAAAAACGCAGCTGCCGCGTCACCGCCACGAAGACACGGCGCGCAGCGGGCAC GAGCCGAGCTGGAGACCGCGCCGCCCGCCCTGGGTAACCTGGGAAGCTGAGGGGAGTCAGTGGCGCCTGG AGAGGACCCCATAGCGGTGGCCGTGGCCAGAGGCGGCAGCTCTGGGCGCTTGCTGCTGGAGGAGGCTGCG GCGGTGCGAGCGGCCGAAGCCAAGGCGATTCAGTGCCTCGTGTTCTTAGTTTTTAACCTCTGACTATGCA ATTCTGAAACCTCCCCCATTGGGGGACCAGACAGCCCGATAGATACCTTCCACTCTTCCTTTGCTCCCGC CCTGGTCTCAAGAACTGAAGGATCTCTCTAACGCCTTTCACCATTAAGAGGAAAGCGATGGAGGAGCTGA GCGCTGACGAGATTCGACGGAGGCGCCTGGCACGACTTGCTGGTGGACAGACCTCCCAGCCGACCACCCC GCTTACATCTCCCCAGAGGGAGAACCCTCCGGGACCTCCAATAGCTGCATCAGCCCCAGGCCCCTCCCAG AGTCTTGGTCTCAATGTCCACAACATGACCCCAGCTACCTCCCCCATAGGTGCAGCAGGAGTTGCCCATC GGAGCCAGAGCAGCGAAGGCGTCAGTTCTCTCAGCAGCTCACCTTCCAACAGCCTTGAGACACAGTCTCA GTCCCTCTCCCGTTCCCAGAGCATGGATATTGACGGTGTCTCCTGTGAGAAAAGCATGTCCCAGGTGGAT GTGGATTCGGGAATTGAGAATATGGAGGTGGACGAGAACGACCGAAGAGAAAAACGGAGCCTGAGTGACA AGGAGCCTTCTTCAGGTCCTGAGGTGTCTGAAGAGCAAGCCTTACAGCTGGTCTGTAAGATCTTCCGTGT CTCCTGGAAGGACCGCGACAGAGATGTCATCTTCCTTTCTTCTCTCTCTGCGCAGTTTAAACAGAACCCA AAAGAAGTGTTCTCTGATTTTAAGGATCTGATTGGCCAGATTTTAATGGAAGTACTGATGATGTCAACTC AGACACGAGATGAAAATCCATTTGCCAGTCTGACAGCCACATCCCAGCCCATCGCCACGGCAGCTCGGTC ACCAGACCGCAATCTCATGCTGAACACTGGCTCCAGTTCAGGAACAAGCCCCATGTTCTGCAACATGGGC TCCTTCAGCACCAGCTCGTTGTCTAGTTTGGGAGCCTCTGGTGGAGCAAGTAACTGGGATTCGTACAGTG ACCATTTCACCATTGAAACCTGCAAAGAGACGGACATGCTGAACTACCTCATCGAGTGTTTTGACCGAGT TGGAATAGAGGAAAAAAAAGCACCAAAGATGTGCAGCCAGCCAGCAGTCAGCCAGCTTCTGAGCAACATC CGCTCGCAGTGCATCTCCCACACCGCACTAGTGCTACAAGGTTCCCTCACACAGCCCAGGTCCTTGCAAC AGCCATCCTTCCTCGTGCCGTACATGCTCTGCAGGAACCTCCCGTACGGCTTCATTCAAGAGCTGGTGAG GACCACTCACCAGGATGAAGAGGTGTTCAAGCAGATCTTTATCCCCATTTTACAAGGCCTGGCGCTGGCT GCCAAAGAGTGCTCCTTGGAAAGCGACTACTTTAAGTACCCCCTCATGGCACTGGGTGAACTCTGTGAAA CCAAGTTTGGGAAGACACACCCTATGTGCAATTTGGTCGCTTCTTTACCCCTGTGGTTGCCGAAGTCCTT AAGCCCTGGTTCTGGGCGGGAGCTGCAGAGACTCTCCTACTTAGGGGCCTTCTTTAGCTTCTCAGTCTTC GCAGAGGACGATGCAAAGGTGGTTGAGAAATACTTCTCAGGGCCTGCCATTACCCTGGAGAACACACGCG TGGTCAGCCAGTCACTACAGCATTACCTGGAGCTGGGACGGCAAGAACTTTTCAAGATTCTACATAGTAT TTTGTTAAATGGCGAAACTCGTGAAGCGGCTCTCAGCTACATGGCGGCCCTTGTCAATGCCAACATGAAG AAAGCCCAGATGCAGGCAGATGATCGGTTGGTGTCTACAGACGGGTTTATGCTGAATCTCCTTTGGGTCC TGCAGCAGCTAAGTACAAAAATCAAGTTAGAAACAGTTGACCCCACTTACATATTCCACCCAAGATGTCG GATCACTCTCCCGAATGATGAGACACGGATAAATGCAACGATGGAGGATGTGAATGAGCGGCTGACAGAG CTCTATGGTGATCAGCCTCCATTTTCTGAGCCAAAATTCCCTACGGAATGTTTCTTTCTCACCCTGCATG CTCATCACCTCTCTATTCTGCCTAGTTGTCGTCGCTACATTCGCCGACTTCGAGCCATCCGCGAGCTCAA TAGAACCGTGGAAGATTTGAAAAATAATGAAAGCCAGTGGAAAGATTCCCCACTGGCCACCAGACACCGT GAAATGCTGAAGCGCTGTAAAACTCAGCTTAAGAAACTGGTACGGTGCAAGGCCTGTGCTGACGCTGGCT TACTTGACGAGAGCTTCCTAAGAAGATGCCTGAATTTTTATGGCCTTCTCATTCAGCTGATGCTGCGCAT CCTGGACCCTGCATATCCTGACGTAACACTGCCTTTGAATTCAGAAGTCCCCAAGGTATTTGCAGCATTG CCTGAGTTTTATGTCGAAGATGTTGCTGAATTTTTATTTTTTATTGTACAATACTCTCCTCAGGTGCTTT ATGAGCCCTGCACTCAGGACATTGTCATGTTCCTCGTTGTGATGCTGTGCAACCAGAACTACATCCGAAA CCCTTACCTAGTGGCCAAGCTGGTGGAGGTCATGTTCATGACCAACCCTTCTGTCCAGCCGAGAACTCAG AAGTTCTTTGAGATGATTGAGAACCATCCTCTCTCTACCAAACTGCTGGTGCCGTCCCTCATGAAGTTCT ATACAGATGTCGAGCATACTGGAGCCACCAGCGAGTTCTATGACAAGTTCACAATTCGCTATCACATTAG CACTATTTTTAAAAGCCTTTGGCAAAACATAGCTCACCATGGCACCTTCATGGAGGAGTTCAATTCTGGA AAGCAGTTCGTTCGGTATATCAATATGCTAATAAATGACACGACCTTTTTACTGGACGAAAGTCTGGAGT CTCTGAAGCGGATCCATGAAGTGCAAGAAGAGATGAAGAACAAAGAACAGTGGGACCAGCTGCCTCGGGA TCAGCAGCAGGCCCGGCAGTCTCAGCTTGCTCAGGACGAGCGTGTTTCCCGTTCTTATCTTGCCCTGGCA ACTGAAACCGTGGACATGTTCCATCTCCTCACCAAGCAAGTCCAGAAGCCGTTCCTGAGGCCAGAGCTTG GTCCCCGATTAGCAGCCATGCTGAACTTTAACCTGCAGCAGCTCTGCGGACCCAAGTGCCGGGACCTGAA AGTTGAAAACCCAGAGAAGTATGGTTTTGAGCCAAAGAAGCTGTTGGACCAACTGACAGATATTTATTTA CAATTGGACTGTGCTCGCTTTGCTAAAGCCATCGCCGATGACCAGCGATCCTACAGCAAGGAACTGTTTG AAGAAGTCATTTCAAAGATGCGGAAGGCGGGAATCAAATCCACCATCGCAATAGAGAAGTTTAAACTTCT TGCAGAGAAAGTGGAGGAAATCGTGGCAAAGAATGCGCGGGCAGAAATAGACTACAGCGATGCCCCGGAC GAGTTCAGAGACCCTCTGATGGACACCCTGATGACCGATCCCGTGAGACTGCCCTCTGGCACCGTCATGG ACCGTTCTATCATCCTGCGGCATCTGCTCAACTCCCCCACCGACCCCTTCAACCGCCAGATGCTGACTGA GAGCATGCTGGAGCCAGTGCCAGAGCTAAAGGAGCAGATTCAGGCCTGGATGAGAGAGAAACAGAGCAGT GACCACTGAGGCCCCATCGCTCCTCCGCTGGAAGCCGCCACGCCAGCCAGCCAGGCCGCAGCAGCATCTG CCTCGAGGCTGCAGCTCCAGCCTGTAACCAGATGTGGCCAGATCCTGAGAGCCCACCCGTGTGACCAAAC AGTGGAGATCTGAGGGGATGACGAGAAGAGGATTCCTGTACATATATTTAAGTGACAACAGTCAAAGCTT GAGGGACGAGTTTACGATGGCTTGTGTAGTAAAGCGCACCCTTCGCCTGTCGCTACCTGGGGCTGTGGCA GCGGAAGTGTTTAGTGACAAGTGGGTCTGGGCAGCTTCCCCTGAGCTTACGTTTTGTTTTGTTTTGTTTT TTCGATGTGAATAGAGAACTCGGACATTTTGCTGCTAAACAATCTCCCCTCCCTCACCCTACTTGCACTG CTGTGGGTTTTTTAAAGAGAAACAGAACCAGACTCCTTTCCCGGCCCTCCAAACATGTATTCTGTGCGAT AACTTGTGCCTGCGACAAGTGTTAATCTTGGGATCGTATTTTTATATCATATTCACATACTTGTTTTTTT AATTGGTGTTAGATGACATGATTAATAAAAAAGGCAAGATATTTTCAGAATTTGAATTTCAGTTTTTTTT TCTTTTGAAATAGCCCTTTTAAATTTTTTTATTATAAACAAAATGAAGAGAACCCTGTGAGCCTGGTCCT TCTAGCAACCCTCAGTTAAGATCCTGAGGGACAGGTGCAGCAGCAGCTGGCTGGAGTGGATCAGGACTCC TGTGACTCGTGGGGCTCGGGGGAGGTGAGGGGTGTGTTTGGTTTTTTGTTGTTTTGTTGTTTTGTTTTAA TCCTATGTGACAATTCCAACTATGCGGTTACCCTCTGAGGATAAGTTCCTCAGCCCGGGGTTCTTTTATA TGCTGTGTCCTTTTGGGGTTTGACCTTACCAAAGCAAAGAAACAAAAAACAAAAAAAACAAAAAAAAAAA AGGGTGCAAGAAATGTGGAAATCTGTCTGCTTGTTTACATGGCCCGACAACACCACCTAGGCGCACGCGA GCAGAAGCCCTCTCTGTATTACCGCTCCCTACGCAACATACTTGAATTCTTGAGTTCCCTTTGGGGTGAG AAAGGATTTGAAACCATAAAGTGTTTTAAAGAAATGAACTCTTCAGAAACAGACTTCCTCCTCTTTTCCT TTCTCCTCTGCTCCACAGCCTGTGCTCTGTTCAGTTCCCAACACAATCGACAATAAACGGTGATGCCCAT TCGGAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6405514 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6405514 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14776 same embryo
	EMAGE:14774 same embryo
	EMAGE:14773 same embryo
	EMAGE:14775 same embryo
	EMAGE:14772 same embryo
	EurExpress:euxassay_005752 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source