EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14772

Rab8b RAB8B, member RAS oncogene family ( MGI:2442982)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005749. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005749_01	euxassay_005749_02	euxassay_005749_03	euxassay_005749_04

euxassay_005749_05	euxassay_005749_06	euxassay_005749_07	euxassay_005749_08	euxassay_005749_09

euxassay_005749_10	euxassay_005749_11	euxassay_005749_12	euxassay_005749_13	euxassay_005749_14

euxassay_005749_15	euxassay_005749_16	euxassay_005749_17	euxassay_005749_18	euxassay_005749_19

euxassay_005749_20	euxassay_005749_21	euxassay_005749_22	euxassay_005749_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14772_wholemount_strong.wlz

14772_wholemount_moderate.wlz

14772_wholemount_weak.wlz

14772_wholemount_possible.wlz

14772_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14772_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8753
Entity Detected:	Rab8b, RAB8B, member RAS oncogene family ( MGI:2442982)
Sequence:	sense strand is shown >T8753 CTGCTTTCCACCGCCTCCTTGCTCTTGGCGGTCAGAGAGGGCCGGAGTGAGAAGATGGCGAAGACGTACG ATTATCTGTTCAAGCTGCTGCTCATCGGCGACTCCGGCGTTGGCAAGACCTGCCTCCTGTTCCGCTTCTC AGAGGACGCCTTCAACACCACCTTTATCTCCACCATCGGAATTGATTTTAAAATCAGAACGATAGAACTC GACGGAAAGAAAATTAAACTTCAGATATGGGACACGGCGGGCCAGGAAAGATTCCGAACAATTACGACAG CATACTATAGAGGAGCCATGGGAATTATGCTGGTCTATGACATCACCAATGAAAAATCCTTTGACAATAT TAAAAATTGGATAAGAAACATTGAAGAGCACGCCTCTTCAGATGTTGAAAGAATGATCCTGGGTAACAAA TGTGATATGAACGACAAAAGACAAGTGTCGAAAGAGAGAGGGGAGAAGTTAGCAATTGACTATGGGATTA AATTTTTGGAGACAAGTGCAAAGTCGAGTACAAATGTAGAAGAGGCATTTTTTACACTTGCACGGGATAT AATGACAAAACTCAACAGGAAAATGAACGACAGCAATTCATCAGGAGCAGGTGGGCCAGTGAAGATAACA GAGAGCCGGTCTAAGAAGACCAGTTTCTTCCGCTGTTCTCTGCTTTGATGAACTCCTCATTTCTGAGAGA CTGCAGAATACCTAGAGGGCCTTTCCCTGCCTCTCTGAAAGCACAGGCTACCCAGCCTCAGAGTCACTTC GCCCGGCTGCTGCGGAGAGCACCCCCGGAACTCACACAGACCACCAAGTGGATTCCAGCCTTGTGGCCTA GCAAAAGAGCGGACTCCTTTCTTTAAAAAGCAGAAGCAGTGATGTGGAGACACACATAGGACCTAACTGG TTTTTCCTTGTTTTACTGCCTGTCCTGGAAGCTGCTGTCTTTCTTTGCACATGCGTGTCAGCCTTTCCCT GGTACAGCGTAATCTTAGACTCTTAACTGAGCACTTTAGTGGCATAAAGTTCTACACTTGTATATTTGGG GATGCTTAAAGACAGCAGAGCATTAAACAGAGGTTGACCTGCTACAATTAAAGGATGGTTAGCCTGGGTC ACATGGCCAAAAATTCTTCTGTTGGTAGATTTATTGAAGGGCTTTGATTTATAGTATTCCTAATTTGATA AATGTTCAAAATTTATAAAAATGCCCATCTAGCCAGAGGCCATGGCATTGCTGTCTTTTCAGGGAGCAAT CAGCCATGCTCATCCCTGAGTAGCTGTGTCCCCTTTGAAGTTGCCTGTCCTGCCTCCTCCATGGACTCAG CCTCTGGAAAGCACTGCAGCCTCACTGTTCAAGGGTTCTGTTTGCTGTCCCTTCTCCTGTGTGACTTGCT GGCGTTATTTACTTATCTAAAGCCCATAGTATGTGAGCATTGCTGTCTGCCTGTGCACTTCATGAGCAGC GATCTCACATAGGTAGCGACGTGGTCATCACAGCTGTCTGTCCACATCCACCTATGGTCTTGTCACATTA GAGTATGCAGTACATTTTTTCTGCGTTTCACCCACTAGCTTATATCACCCAGTTCTTAACCAGAATCCCC ATCTGCGAGAGTTAACAAAGGTTAGATTTAGATTTTAGTTTGCAGAAAACATACGTAACATATACAGAGA GAGATTAAACATATAATTTTAAAAGACAGCTATTCGTAAGGCTGAAAGAAATGACTCTTTGGGTTTTTGT TTGTTTGCTTGTTTTGTTTTTAAGTAATTAGACCTAGCACATCCTCCAGAAATCTTGTCTTTTCATTAGA GCACATATGATTCAGGTTTCAGCTTTCAAATAGCCATTGCTGGGTATCTAAGACCTAGCCCTGTTTGCTT AGGTTTGTCATTAGAGAAGTGGTGTGGCCATCCAGGCCAAAACACACCATAGGCGTCACTGCCTTTCTCA CCAAATGCTGATTCTGATGTTTCTTCTGTGGGTGAAGCCACTGCACTGGGGACGGGGAGGGTGTTCAGTG TTAACTTTTGTGCCAACTATAGATCATAATGTCATTGCTATCCTAACACAGAGAACATAAAAACAGGTGA CAGTGGCAGGCACTTGTACCTTAAGATGGATGTGGATAGCTGGTTATACAGACAATTCTGCCTCAGATTA AGATCTCTGGTTTCAGTGTTTGGACCAGTCAGGCCTTCCCATCTGTTGGGAAGCTCAGAAACATTCCAGT GGCTGCAAGTATCCCAAAGTTATTTTTGGTGGATATGCTGACTCTACTGTTGACAAACTTCAGCCGTTTC TTCTTTTTCTTTAGTTACCTATTGGCTTAAAATCACACATTGCTACCCCCGTGCAAGCAGTTTACTCATT GAGAACTTATTACAAGTCTAACATTGTGAAATTAAATACATATTTGAAAAGAACAAGTATTTTCTTAGAA ATGTCTTATCACTACTTTTCTGGAAAAAAAGTTCAAGCAGTTACATTGGTCACAAAGTACTTTGCAAAAA GCACAGGCCGTGACACCACATACGAATGTAATTCTCTTAGTATTTACCTCTGACTGTTTGGTGTCTTAGC ACTTCGGTTTCTATCTCAGGGGTTGTCTGCAAACCAAAACTGACATGTTCTGTGGTCAGCTTTTTCTTTT CTTTTCTTTTCTTCTTTTTTGTTTTTGTTTTTTTTTTAAACAAACAAAACCAGAATGCTTTGCTTTTGAT ATCTCCACCCTTCTCGTGTCTTTTGTGGGTTAATAACTAGTCTCCATGAACTTCTCTTTGAAAGAGCCTA TAAATGTTAGGTGAGTCTGAAAGTGCTCTTTGAAGCAGCAGCAATCGGGGAGTATATCCTTGTTATATAG AAATACATTGTCCTTGCTATTTTCTTACATTTAACTTTGCTAAATTGTATATAATTTGAGCTCAGACCAT AGGAAAGTCACTTTCTGCATGGTAGAAACAACCCAACAAATAGTCTACTTTGCTTAATAATGTACATATG TGCATTATTTTTTCTACTTGTAGATGAATTTAGTGACAGAGAATTGTCTGTTCCCTGCTGAGGCTGAAGA TATCTAGAAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGGTGAGAGAGA GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCGCCTTTCTGTGGTCTT CTGGATAGGGTACATAGGGATTTTTGCAGCAGTATTAGTGGTTCAGTGGCTGCACTTTATTATTGTGCTA ATTTTTGACGATTGGACTCGAAATTTTCAACTAGAGCCTGAGGGTATCAAACAGTGAAATGTACTGTAAC TAAGTAGCATGTGAATCAGTTGATTGTAAACTGTTCCACTGGGAACTTATTCTAGCCTCCTGTAGTTCAG GCAGATGATAACAAAGTGATCTACCTGTGTACCAGTTGCTGCCATAGGCCTTTTTACTGTTCATGTGGAC ATTTTATGCAGTCTTTGTTATAAATTTTCTGAAGACTATGCTCTTTACTTTGAAGGACTATTTCTTTTAT TATACCTCATTTAGCTAACTGATATTCTAATAACCAGTAGAAAACAGTGAGCCCGCCTCCAGGCATTCAA CACATTTAGGTTGCATGTTTTGTTTTGTCTTGTTTTGAACTGGAGGAAAAGAGAAATACTTTCGAATAAA GCATGCATGTCAATACCCAGTAGCATACAAAAATATGACTTTCCTGTCCATCCATTAATGAATTTAGTTG TGCAATATAATAGGTTGCCCTGCAGTTTATGAGTAAGAAATTGTAACTGCATTTTTCAAGCTCCAAATGC TGAGATCTGTATTATGGCTTATAAAGTGTCTTCCTATGAGCAGTAAAAGGACAAATTAGGCAGACTTTCT TTTCAAGCTTGTGACTTAGATTCTTTTATAAATATAGTTCTTAATCTTTAAAGATGAGATTCTAAGCCTA GAATTTTAAAACCACATTTCATTATGGTGGTTTACTGATCTCAGATTTTCTACAATTCAATTTTTTCAGA CTTAAGAACAGCTAAACAAAACCTTCCTCTAGCTTTTTCATCACATCCAGTGGCAGAGAGGAGTGTCTGC GCATGAAATCCCGGGATTCCGGAGGACATAGCAGGTCAGGGTACACTGATAAAAGTTATGAATTTCCTGT CCGTTAGAAAGAAGCTAAACGACTGAGCATAGAGATGGAGAGCAACGAGCATTTGTTTTAAGGATTTTCC GGATTTTACTGTGTATCTTGAAGTGTGTGCCATATGCCAAGAACTAACAGAACTTTGTGTCTCCTCTTTG TACCTTTTTCATTTTTTAAAGTGAACTTATGGTCGTTCATTCCTGTGGCCTTACGGATGGGTTTGTCTTG ACTGTTTGCAACTGATGCTGCAGTTCCCTTAATGCAAAATGCCATGAACTGTCTGAATACAGTCAGGCCC CACCATGGGAACTATGGCGTTTCTTCATAGACTTCTACTTGTGTGCTTAAACATTTTCTTTTGCTTCAGT GGCTTACTGTGGGAAGCTCCTGTGGATAGTATGGACCTGCCATGTGGCTAGTCTGTTGAAGCCAGTTCAT TAACTGTACTGATACTGATGTTGTGTTTTTTAAAAATGAATTTTATTCCAGGTGAACTTTTTTTTTTACA ATAATGTTCATCTAAAATAAAAACTACATGTTGAAAAATGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6405462 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6405462 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14776 same embryo
	EMAGE:14774 same embryo
	EMAGE:14773 same embryo
	EMAGE:14775 same embryo
	EMAGE:14771 same embryo
	EurExpress:euxassay_005749 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source