EMAGE mouse gene expression spatial database

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EMAGE:14778

Slc18a1 solute carrier family 18 (vesicular monoamine), member 1 ( MGI:106684)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005750. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005750_01	euxassay_005750_02	euxassay_005750_03	euxassay_005750_04

euxassay_005750_05	euxassay_005750_06	euxassay_005750_07	euxassay_005750_08	euxassay_005750_09

euxassay_005750_10	euxassay_005750_11	euxassay_005750_12	euxassay_005750_13	euxassay_005750_14

euxassay_005750_15	euxassay_005750_16	euxassay_005750_17	euxassay_005750_18	euxassay_005750_19

euxassay_005750_20	euxassay_005750_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14778_wholemount_strong.wlz

14778_wholemount_moderate.wlz

14778_wholemount_weak.wlz

14778_wholemount_possible.wlz

14778_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14778_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

moderate

regional

moderate expression: see section 06 07 08 13 14

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 07 08 11 12 weak expression: see section 13

cervical ganglion

moderate

regional

moderate expression: see section 07 weak expression: see section 13 14

thoracic ganglion

strong

regional

strong expression: see section 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7527
Entity Detected:	Slc18a1, solute carrier family 18 (vesicular monoamine), member 1 ( MGI:106684)
Sequence:	sense strand is shown >T7527 GAGAACTCGGAGCGGGTAGGGCAATCATCAGGAGCCCACTTAGCCCATGGGAAGGTTCCAGTCGGTGCTC TCACGCTCGGCCCACCGAAGTCACGGAAAAGCTCACGCAAAGCTGGAGAGGTGCTTGTGAACTGAGCGAT GGGGGAAATGAATCCCATTCCCTCCCCGCTGGCTGAACAGAGAACAGAGTCTCCGCTGCCTTGCCAACAG CTGCCAGTTCCTCTCGGAGTGCCTCACATCCAGACGAGCTGTAAGTGAGCTTCACTAGACCAGACAGACT GCCCTTTCTCCTGTAAAGGTGACAGAAGACCACATCTATCAAGGGGCCTTCCTAAGCACCCGGAGGAGAA GGCCTCCCCGCCATCCCACTCCCTACCCAGCCCAGCCTCCTGCAGCCCTTGCCATGTTCCAGGTTGTTCT GGGTGCTCCTCAGCGACTGCTGAAGGAAGGGAGGCAGTCCCGGAAGCTGGTGTTGGTCGTGGTGTTTGTG GCTCTACTCCTGGACAATATGCTGCTCACTGTGGTGGTGCCCATTGTGCCCACCTTCCTGTATGCGACAG AGTTCAAAGACATCAACTCTTCTCTGCTTAGGGGTCCTTCTGTAAGCTCCCAGCAAGCTCTCACCTCTCC TGCCTTCTCTACCACCTTCTCCTTTTTTGACAACACCACCATGACTGTAGAAGAACATGTACCTTTTCGT GTAGCATGGATAAATGGTACCATCCCTCCTCCAGTCACCGAAGCTGGCTCAGTACCTAAAAACAACTGCT TGCAAGGGATAGAGTTCTTGGAAGAAGAAAATGTCCGGATTGGGATTCTATTTGCTTCAAAGGCTTTGAT GCAACTTCTGGTTAACCCATTTGTAGGACCTCTTACTAACAGGATTGGCTATCACATCCCCATGTTTGTT GGCTTTATGATCATGTTTCTCTCCACACTAATGTTTGCTTTCTCTGGCACCTATGCCCTGCTGTTTGTGG CCCGAACTCTCCAAGGCATTGGATCTTCATTTTCATCTGTTGCAGGGCTTGGGATGCTGGCCAGTGTCTA TACGGATAACTACGAGAGAGGAAGAGCCATGGGAATTGCTTTGGGGGGCCTGGCTTTGGGACTTCTGGTG GGAGCACCTTTTGGAAGTGTGATGTATGAATTTGTGGGCAAGTCCTCACCATTCCTCATCTTGGCCTTCC TGGCACTTCTGGATGGAGCTCTCCAGTTTTGCATCCTATGGCCTTCGAAAGTGTCTCCTGAGAGTGCCAT GGGGACTCCACTTTTGACACTTCTCAAAGATCCTTACATTTTGGTAGCAGCAGGTTCCATCTGCCTGGCC AACATGGGAGTCGCCATATTAGAGCCCACGCTACCCATCTGGATGATGCAGACCATGTGTTCCCCGGAGT GGCAGCTAGGTCTGGCTTTTTTACCTGCCAGTGTGGCCTACCTCATTGGCACGAACCTTTTTGGTGTGTT GGCCAACAAGATGGGTCGGTGGCTGTGCTCCCTTGTTGGAATGGTAGCTGTAGGTATCAGCTTGCTCTGT GTACCTCTGGCTCACAATATTTTTGGTCTTATTGGACCCAATGCAGGCCTTGGCTTTGCCATAGGCATGG TGGATTCCTCTCTGATGCCCATCATGGGATACCTGGTGGACTTACGCCATACCTCTGTGTATGGGAGTGT CTACGCCATCGCTGATGTGGCTTTTTGTGTGGGCTTTGCTATTGGCCCATCTACCGGGGGTGTTATTGTA CCGGTCATCGGCTTCCCTTGGCTCATGGTCATCATTGGAACTATCAACATCATCTATGCCCCTCTCTGCT GCTTCCTGCAGAACCCACCAGCTAAGGAGGAGGAGCTTGCAATTCTGAACCAGGAATGCCCCACAGAGAC CCAGATGTACACAATCCAGAGGCCCACGAAGGAGTTTCCACTGGGAGAGAACAGCGATGATCCTGGCAGT GAGGAGTAGCTGTAGAGGGTGATATCTGGGCCTCATTCCAGGTGACCCCATGAGGCGTGAATCTCAGTGT CCCAGGGGACCAGATCATCACAGATTCAATGGGTCTCACTTTCCTCTCCTCTGGGCAACTCCCTTCCTTC CTTCTTATTGATAGCTTGTTCTTACTCACCTGTAAGTACAACCCACCACTCTCCCAATGTGCTTTGACAC CACCAGTGGCCCATTTTTTGTGGGAGGACAGTGCTACTTCCTGCCAGGCCAAAGCGAAGCTGATTAAAGC TGAGTTGAGACAAGCTCTGCAAGGGGTGACTCACTTCCTGCAGGCAGTGCTGAACAACGTGCCTGTGAAA CCAGGGCTACAAATGGCAAGCCTTCCTTTCTTCTCTGATTTTTTCTAACATATTACCAATATGTCTTAAA ATTTGACCTGTCTCCTGTGATATATTTTCCCCTTATTTTTTCCAGGTGAGCTTCCATATGTAGGTTGTTG TTTTTTTTTCTTTCTTATTTTATGTGCTAAAATTACTTTAACTTCATCAAATGAAGCCTTCTGGCTTTCC TCATCATCCAATCAAAGGGAAATAAGCACTTTTCCTAGGTCTGTGTTATGTCTGCTCTGATGATTTCTCA TGTATGTTTCTAGCTTTCCCCAACCTCTTAAACTCTTATAAATAAAAGTGGTTGAAGTACAGAAAAACAA ACAAACAAACAAACAAACAAACAAGCCAATGAAACAACAACAGAAACCCCAAATAAACAACATTGCATTA GAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4239930 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4239930 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14777 same embryo
	EMAGE:14781 same embryo
	EMAGE:14779 same embryo
	EMAGE:14780 same embryo
	EurExpress:euxassay_005750 same experiment
	MGI:4828122 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source