EMAGE mouse gene expression spatial database

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EMAGE:14802

Prkag2 protein kinase, AMP-activated, gamma 2 non-catalytic subunit ( MGI:1336153)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005748. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005748_01	euxassay_005748_02	euxassay_005748_03	euxassay_005748_04

euxassay_005748_05	euxassay_005748_06	euxassay_005748_07	euxassay_005748_08	euxassay_005748_09

euxassay_005748_10	euxassay_005748_11	euxassay_005748_12	euxassay_005748_13	euxassay_005748_14

euxassay_005748_15	euxassay_005748_16	euxassay_005748_17	euxassay_005748_18	euxassay_005748_19

euxassay_005748_20	euxassay_005748_21	euxassay_005748_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14802_wholemount_strong.wlz

14802_wholemount_moderate.wlz

14802_wholemount_weak.wlz

14802_wholemount_possible.wlz

14802_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14802_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7499
Entity Detected:	Prkag2, protein kinase, AMP-activated, gamma 2 non-catalytic subunit ( MGI:1336153)
Sequence:	sense strand is shown >T7499 CCCAGCACAAGACTTCTGCCACGCGTAACCAAAAGTAGCACGGGCCGAGACTCTGCACCCAATGATTCCG TAGGAGAAAGAGGACGCTCCAACCCGGCATTCCGCGGACTGCCAGCCCCTCGGGTGGAGCCCCGCGTGTT ACCTGCGCCACCCGCCCTCCCCGCGGAAGTCCCCACGAGGTGTTTTCAGGGTGGGAGGACAGTCATAAAA AGCGCGTGGCTCCAGTGTAACGGCAGCAGTCCTGGGAGGCAGCGAGGGGACCGAACCCCGGGGACGAGGC ACCGGAATTAACTTCTAGCTAAAGTTATGGGAAGCGCAGCCATGGACACCAAGAAGAAGAAAGAGGTTTC CAGCCCGGGCGGGAGCAGTGGCAAAAAGAACCCTAGCCTGAAGAGGCGGTCACTGCGAGTGCACATTCCG GACCTGAGTTCCTTTGCCATGCCGCTCCTGGATGGAGATGTGGAGAATTCAGAAAAGCATTCATCTCGGA AGGTGGACAGTCCCTTCAGCTCAGGCAGCCCCTCCAGAGGACTCTTCTCCAGAGGCCCCCAGCCCCGGCC CTCCAGCCCTGTGTCTGCTCCCGTGAGGCCCAAGACGAGCCCTGGCTCTCCCAAAACTGTGTTCCCGTTC TCCTACCAGGAGTCCCCTCCACGCTCTCCACGCCGGATGAGCTTCAGTGGGATCTTCCGCTCCTCATCCA AAGAGTCTTCGCCCAACTCCAACCCGTCTACCTCTCCTGGGGGCATCCGGTTCTTCTCACGGTCCAGAAA AACCTCCAGCGTCTCCTCTTCTCCGTCAACACCCACCCAAGTGACCAAGCAGCACCCATTTCCCCTGGAG TCCTACAAGCAGGAGCCTGAACGCCCAGAGAGCCGCATCTATGCTTCCTCGTCCCCTCCAGACACTGGGC AGCGCTTCTGCCTGGCCTTTCAGAGCCCAGCGCGACCTCCGCTGGCATCCCCGACATACCATGCTCCTTT GAGAACTGCGGTGCTGGCGGCGGCCCCGGGACCCGCGGAAGCCGGCATGCTGGAGAAGCTGGAGTTCCAG GAGGAAGAAGACTCAGAAAGTGGCTTTTATATGCGATTCATGAGGTCACACAAGTGTTATGACATCGTTC CAACCAGTTCAAAGCTTGTTGTCTTCGACACTACGTTGCAAGTCAAAAAGGCCTTCTTTGCCTTGGTAGC CAACGGAGTCCGTGCAGCGCCGCTGTGGGAAAGTAAAAAGCAGAGCTTTGTAGGAATGCTCACGATTACA GATTTCATAAATATCCTACACAGATACTATAAATCACCCATGGTACAGATTTATGAATTGGAAGAACATA AGATTGAAACGTGGAGGGAGTTGTACTTACAAGAAACCTTCAAGCCTTTGGTGAACATCTCTCCAGATGC GAGCCTCTTCGATGCTGTATACTCGTTGATCAAAAATAAAATCCACAGATTGCCAGTTATTGACCCTATC AGTGGGAACGCACTTTATATACTTACCCACAAAAGAATCCTCAAGTTCCTCCAGCTTTTTATGTCTGACA TGCCAAAGCCTGCCTTCATGAAGCAGAACCTGGATGAGCTTGGAATCGGAACGTACCACAATATTGCCTT CATTCACCCAGACACTCCCATCATCAAAGCCTTGAACATCTTCGTGGAGAGACGGATATCCGCATTGCCT GTGGTGGATGAGTCAGGAAAAGTTGTAGATATTTATTCCAAGTTTGATGTAATTAATCTTGCTGCTGAGA AAACGTACAATAACTTGGACATCACAGTGACCCAGGCCCTGCAGCACCGCTCGCAGTATTTTGAGGGTGT GGTGAAGTGCAGTAAGCTGGAAACACTGGAGACCATCGTGGACAGGATAGTGAGAGCCGAGGTCCATCGG CTGGTTGTAGTGAATGAAGCAGATAGCATTGTGGGTATTATCTCCCTGTCAGACATCCTGCAAGCCCTGA TCCTCACACCAGCAGGTGCCAAACAGAAGGAGACAGAGACTGAATGACCGCCGTGAATGTAGACGCCCGC GCAGGAGAACTTGAACAAAGTCTCGGGGTCACGGTTTGCCTCATGAACACTGGCTGCAATAGAAGAGAGT AAAATGGCTAAGAATGTATACCAGGGTTTAGCGATGGGTATTTTTCCAGTGATTTGTAAGTATAGGAAAA GATTTTATGTGCTTGAAGATTCAGGCTTGCGTTCAAAGACTGCCCTCAGACCTGTCTGAAGGATTTTCAA TGCTGTATGTCATTAAAGTGCACTGTGTCCTGAAATAGTACTACTTTTCATTTCAAAGAAGTCACTGGGA TGGAACAGGTGACAGAAAGTGAGTGAACGGCACGTCCAGATCACGGTGCCTTATGTCCAAACGCAATATG TCACCGCCGCTGCCGCACGCCCCAAAGTGCTGCTGAAGAGACACCCAGCCCAAGTGTGAGCATCTTCAGA CCTTTTACCCAGTCTCTTCCTTTTCACTTAATTGGTTGAAAAGTTTTAATTTGAGTACAGGCAACTGAAA ATGTGATGTGATGCCTCTAAGCGCTCTGCATCCGTCCTGTTCTGGCTGCCGTGTTAGCGAGGCCGGCTTG GCAACAGCGCTTTATTTATTTTTACTCTCATGCAGGTTTCAACCTACATCCTTTGGTGAGTAGACGTCAC CATACCTACGAATAAAACCTTGATTGTTCTCTCAAGTGGAAAATCTCTGGAGATTTTAGTTTGGATCTGG AAAGGCTATGCCTTCCAAACAGCCACTGTAGGGTCTTCTAAACGTTTATGAAAACAGGCTGTGGTCGCAG GGAAGAAGTAACGGAAGACGTCTTTTTTTTTATTGTGTGTTGAAATGTAAACTCATGATGTTTGTGTGAC CGCTGACAAGACGCTTGTAACTTTTATTGTGACACATAAAACAGTATTGTCATGCAGTTGAAGGGAAACT CTTTCCCCTCCCACACCCCACAACCCCCACAGCCCCCAGTGTCAGTGCTCTGAAAATGTTGACACTATAT ATCTGTGTGTGTATATATGAGCAGAGTTTGGGGGTTTGTTTTGGGGTTTTGTTTCGTTTTGTTTTTCAGA TTGAAAATTAAAATAAATCCTGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4238045 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4238045 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14805 same embryo
	EMAGE:14806 same embryo
	EMAGE:14804 same embryo
	EMAGE:14807 same embryo
	EMAGE:14803 same embryo
	EurExpress:euxassay_005748 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source