EMAGE mouse gene expression spatial database

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EMAGE:14803

Ubqln4 ubiquilin 4 ( MGI:2150152)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005753. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005753_01	euxassay_005753_02	euxassay_005753_03	euxassay_005753_04

euxassay_005753_05	euxassay_005753_06	euxassay_005753_07	euxassay_005753_08	euxassay_005753_09

euxassay_005753_10	euxassay_005753_11	euxassay_005753_12	euxassay_005753_13	euxassay_005753_14

euxassay_005753_15	euxassay_005753_16	euxassay_005753_17	euxassay_005753_18	euxassay_005753_19

euxassay_005753_20	euxassay_005753_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14803_wholemount_strong.wlz

14803_wholemount_moderate.wlz

14803_wholemount_weak.wlz

14803_wholemount_possible.wlz

14803_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14803_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7481
Entity Detected:	Ubqln4, ubiquilin 4 ( MGI:2150152)
Sequence:	sense strand is shown >T7481 GAGAGAGAGAGAGGAGGAGCCGGACGGGCTGGCGGGCGGCCGGGCGGCGGCATGGCGGAGCCGAGTGGGG CTGAGACGAGGCCCCAGATTCGGGTCACCGTCAAGACCCCCAAGGACAAGGAGGAGATCGTGATCTGCGA TCAAGCCTCGGTCAAGGAGTTCAAGGAGGAGATCTCCCGGAGGTTTAAGGCTCAGCAGGATCAGCTGGTC CTGATCTTCGCAGGCAAGATCCTCAAGGATGGGGACACCCTGAGCCAGCACGGCATCAAGGATGGGCTCA CAGTCCACCTAGTCATCAAAACCCCTCAGAAGGCTCAAGATCCAGTGACTGCTGCTGCTTCTCCTCCATC CACTCCTGACTCTGCCTCTGCACCCTCCACCACGCCTGCCTCACCTGCCGCCGCCCCTGTCCAGCCCTGC AGCTCTGGCAATACAACTTCAGATGCTGGCAGTGGAGGGGGGCCCTCTCCAGTGGCTGCAGAGGGGCCCT CAAGTGCTACTGCATCGATCCTCTCTGGCTTCGGAGGCATCCTGGGACTGGGCAGCCTGGGCCTGGGCTC CGCCAACTTTATGGAGCTGCAGCAACAGATGCAGAGACAGCTCATGTCCAATCCTGAGATGCTGTCGCAG ATCATGGAGAACCCCTTGGTCCAGGATATGATGTCAAACCCTGATCTGATGCGCCACATGATCATGGCTA ATCCCCAGATGCAGCAGCTGATGGAGAGGAACCCCGAGATCAGCCACATGCTCAACAACCCAGAGCTCAT GAGGCAGACAATGGAGCTTGCTCGTAACCCAGCCATGATGCAAGAAATGATGCGGAACCAGGACCGGGCC CTCAGCAACCTGGAAAGTGTCCCGGGAGGGTATAATGCCCTCCGCCGCATGTACACGGACATCCAGGAGC CCATGTTCACTGCTGCTCGGGAACAGTTTGGCAACAATCCCTTCTCTTCCCTGGCCGGGAACTCCGACAA CTCATCCTCCCAACCTCTTCGGACTGAGAATCGAGAGCCCCTCCCTAACCCCTGGAGCCCCTCGCCCCCC ACCTCCCAGGCCCCCGGGTCCGGTGGGGAGGGCACCGGAGGATCGGGGACCAGCCAGGTGCACCCGACAG TCTCGAACCCCTTTGGGATCAATGCGGCTAGCCTGGGGTCAGGGATGTTCAACAGCCCAGAAATGCAGGC GCTCCTCCAGCAGATTTCTGAAAACCCCCAGCTGATGCAGAATGTCATCTCAGCCCCTTACATGCGCACC ATGATGCAGACGCTTGCCCAGAACCCTGACTTTGCTGCTCAGATGATGGTGAATGTGCCGCTGTTCGCAG GGAATCCACAGCTTCAGGAGCAGCTCCGCCTGCAGCTCCCCGTGTTTCTGCAGCAGATGCAAAACCCAGA GTCTCTCTCCATCCTCACCAATCCCCGGGCCATGCAGGCCCTGCTGCAGATCCAGCAGGGACTACAGACC CTCCAGACTGAAGCCCCTGGGCTGGTACCCAGCCTTGGCTCCTTTGGGACACCCCGGACCTCGGTACCTC TGGCAGGCAGCAACTCTGGATCTTCGGCAGAGGCCCCCACTTCTTCCCCGGGCGTGCCAGCCACATCTCC TCCCAGTGCGGGTTCCAATGCCCAGCAACAACTCATGCAGCAGATGATCCAACTTTTGTCCGGAAGTGGG AACTCACAGGTGCCGATGCCAGAAGTGAGATTTCAACAGCAGCTGGAACAACTCAACTCCATGGGCTTTA TTAACCGTGAGGCCAACCTACAGGCCCTGATCGCCACAGGAGGGGACATCAATGCCGCTATTGAGAGACT CCTGGGCTCCCAGCTCTCCTGATCCCTTGGCTGGTGCCTCCTGCCTTCCCCACCTCTCAGCGCCAGCCTC TGGCTCCTCTGTGAAGCCCTATCTTCCATGGCCTGTCTTCTTTGACCCCTACGAACAGAGCAGCAAGAAC CGCAGGGTGACTTCCAACTTCCAAGGCCTCTGCCCAACCCAAAGCTCTCCAAGGATTTTGATTCGTTTAC TGTCTCATGAAATCTCTCCCGGTCCTCTTGGAGCAGAGCTATCTAGTGTGCACAGTGTGCCAGTTTGGCC ACATCTCCTTGCCCACAAGTACCTTGGCAACATACATTCTTCTCCAGGGGACTGAGAGGAGGACCCCGCC TGGCGTACCGGAAGTCTTCCGTTGGTACCACTAGAGTTTAGCTAGCCTTACATTACTCCTACCCTCCTCT CCCTCCCTCACCTCAGCCCAACCAGTTTGAATCTCACTCTTTGGCCAGGTCCTGGTGAAACTGTTTTTTT TTTCTCTACCCCTATACTGTTTCATATAGCCTGTCCTTCTGTCCAAAGGCAGCAGTGAGTCAGCAGTCTG GGTAGGGTTATCAAGATCAAACCAGACCAGTGGACACTGGTGCTCTAGAGCCACAGCTGAGAGCTCTCGG AAGCAGGGACCCGGGGAGACAGCCTGGGCAGGAGGCTTGGAAGTTGGAGTTGACAGAGTCTAGAAAGACC AATAGATTTCATGGTGGAGAAGCCAGGCTCAGGCCTGACAGAAGAAAGTCTGATGGACTTGGAACTGAAG ATAGAAGTTCAGCAAAGCTGAACAGTGTGACCCATGAGAAATCAAATTGGCTCCATTGTTTCCAGACATA TTTGCGCTCACGTTCCAATGAACCCAGTCTTCAGGCTCCAGTATTTTGAGCCCCACTCTCCAGAAGATCT GGTGGGGTTTCTCAGCATTGTAGAAAATCTATATGTGTGCGTACATGCAAGTTGGGGAGGTGTGTGTTCT TTGTTGATTTCTGGTTCACAACTTTGTCAACAGAGATAAGTGGGTACAAGGGCCAATCTTGTGGAAGACT ATAGCATTGAGGGAGCCGGGTCCCTGGGCCTCTGGGTATTGGGTAACCAGAAAGCAGATTGAGTAATTCT CTGAGCAATAATGGCTCTGACTGGTACCCTGGCCACCATTGCTTCCTTGGTGAAAGAATAGTGAGGTTTC TCTTCCAGAGCTCCTGCCTTTCAGTTATTTGCTGGTTCCCTCTAGGCCAGAAACTTGTATTTGAGGAGAG GTGGGATGGCAGATGCCTTTGGTATGGAACTGGGCTTTGCTTAACGAGTACTAGAGGAAGCTCTCGCCAC ATTTTCTCTGCTGTGTGGTCCAGGTACTTGGAGGACCCCTGGCTGAGAGACAGGTAAGGAAAGGCATCCT GCCGTAGGTGGGGGACAGTCCCAGACAGTTCAGACATGCCTTCCCTTCCCCCCTTATACCCAACACCCAC TCTGGCCTTTGTAAATAAATAATGTGATTTTTGTTGTGAGATTAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4236887 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4236887 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14805 same embryo
	EMAGE:14806 same embryo
	EMAGE:14804 same embryo
	EMAGE:14802 same embryo
	EMAGE:14807 same embryo
	EurExpress:euxassay_005753 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source