EMAGE mouse gene expression spatial database

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EMAGE:14804

Plvap plasmalemma vesicle associated protein ( MGI:1890497)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005747. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005747_01	euxassay_005747_02	euxassay_005747_03	euxassay_005747_04

euxassay_005747_05	euxassay_005747_06	euxassay_005747_07	euxassay_005747_08	euxassay_005747_09

euxassay_005747_10	euxassay_005747_11	euxassay_005747_12	euxassay_005747_13	euxassay_005747_14

euxassay_005747_15	euxassay_005747_16	euxassay_005747_17	euxassay_005747_18	euxassay_005747_19

euxassay_005747_20	euxassay_005747_21	euxassay_005747_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14804_wholemount_strong.wlz

14804_wholemount_moderate.wlz

14804_wholemount_weak.wlz

14804_wholemount_possible.wlz

14804_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14804_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7463
Entity Detected:	Plvap, plasmalemma vesicle associated protein ( MGI:1890497)
Sequence:	sense strand is shown >T7463 CGGACGCGTGGGTCGACCCACGCGTCCGGACAGTGCGCCCTGTGGACACTGCGCAAATGGGGCTCTCCAT GGACCGCAGCCCATACGCCCGCACGGGGGACCAGCAGCGCGGCTGCTGGTACTACCTGCGCTATTTCTTC CTCTTCGTGTCGCTCATTCAGTTCCTCATCATCCTGGGCCTGGTCCTCTTCATGATCTATGGCAATGTGC ACGCCACCACTGAGTCCAGCCTGCGCGCCACGGAGATCCGCGCCGACAGCCTGTACAGCCAGGTGGTTGG ACTATCGGCCTCACAGGCTAACCTGAGCAAACAGCTGAACATCAGCTTGCTTGTCAAGGAAACAGTCATG CAGCAACTGTTGACTACGCGACGTGAGATGGAGCGCATCAACGCCAGCTTCCGCCAGTGCCAAGGCGACC TGATCACCTACATAAACTATAATCGCTTCATCGCCGCTATCATCCTGAGCGAGAAGCAGTGCCAGGAACA GCTGAAGGAGGTCAACAAGACCTGCGAAGCTTTACTCTTCAAGCTGGGAGAAAAAGTTAAGACACTGGAG ATGGAGGTGGCCAAGGAGAAGGCAGTGTGCTCCAAGGACAAGGAGAGCCTGCTGGCAGGAAAGCGGCAGA CGGAAGAGCAGCTGGAGGCCTGTGGCAAAGCACGCGAAAGGCAACAGCAGGAGCAGCAGGTGACCGAGGA GAACCTGCGCAAGGTGCAGTCCCTGTGCATCCCACTGGACCAGGAGAAGTTCCAGGCGGACGTGCTGAGT GCGTGGCGGGACTCGCTGATCTACCGTACCTTGGAAACCCTGCCCTACCACTACCAGCTGATGCCGGAGT ACGCGTCCCTCCGTCGGACCTGCGAGAGCCTGCCCGGGATCATGACCACCAAGATAGAAGAGCTGGCCCG CGGGCTGCGCGCCGGCATTGAGCGCGTGACCCGTGAGAATGCAGAACTGCGGCGCCAGAAGTTGGAGCTA GAGCGCGCGGCACAGGCGGCGCAGGAGGCGCGGGCGCGCGCCGGGACCGAGGCGCAGGCGCGAGAAACCC AGCTACGGGCGGAGTGCGCGCGCCAGACGCAGCTGGCTCTGGAGGAGAAGGCGGCGCTGCGCGCGCAGCG GGACAACCTAGAGCGCGAGCTGGAGGCGCGTAAGCGGGAGCTGGAGCAACTGCGCACCGAGGTGGATGTG CGCATTAGCGCGCTGGACACCTGCGTCAAGGCCAAGTCGCTGCCAGCCGTCCCGCCGAGAGTCTCAGGCC CACCCCCGAACCCTCCACCCATTGATCCAGCTAGCCTGGAGGAATTCAAGAAAAGGATCCTGGAGTCTCA GCGGCTCCCTGTAGTCAACCCTGCTGCCCAACCCAGCGGTTGAGAACCCAGCTGCCGCAGGACGCTGGGT GCCAGAATCGCCCACCTGTGGATGGGGGCAGCCAGGTGCCCACAGTGCTGGACACCCGCCGTGCCTGCCG GCAGCCTCCACCCCCACGCCTTCTCTGGCACCCCTTCACTGTCCCCTGCATCCCCACCATTCCCCAGACA CTGGATTTAAGGCACACACAGCTGTGAGATGACTTCACATCGACCCCTTGTGCAGTGACCCGGATGGTGC CCCACCCACACATGAAGCACCCACAGCTCAGCTGCCACCCTAGGCAACTCCTCCGGTTTCCTATCACTCT GCTCCTGACCCGGGAGGTGAGAACAGGAAGCCCAGCCTTCAGCTCCCTTGGGAGTTTCCAGCCTCCCTCT TAAAGGCCACTAGGGTTTCCAGATCCTATTTGAGAGTCTCCAGGCCTCCCCTGAAGGGTTCTAGCCACCA CGCCCACAGGATTCCCATTAGGTTTTAAAGTCTTTTCCAGAGTCCGCTGGTTCCCCTCCTCCTCACAAGG AAGGGCCTCAATTGTAGATGAGCGTTCCGGGTGGATCTTAGAGCCCTAGAGGGAGGCTTTTGCTTGTAAC CCCCTAAAGATATTACTGGCACATAATAAATATGAAAGTCCTTTGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4235957 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4235957 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14805 same embryo
	EMAGE:14806 same embryo
	EMAGE:14802 same embryo
	EMAGE:14807 same embryo
	EMAGE:14803 same embryo
	EurExpress:euxassay_005747 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source