EMAGE mouse gene expression spatial database

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EMAGE:14806

Hnf4a hepatic nuclear factor 4, alpha ( MGI:109128)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005745. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005745_01	euxassay_005745_02	euxassay_005745_03	euxassay_005745_04

euxassay_005745_05	euxassay_005745_06	euxassay_005745_07	euxassay_005745_08	euxassay_005745_09

euxassay_005745_10	euxassay_005745_11	euxassay_005745_12	euxassay_005745_13	euxassay_005745_14

euxassay_005745_15	euxassay_005745_16	euxassay_005745_17	euxassay_005745_18	euxassay_005745_19

euxassay_005745_20	euxassay_005745_21	euxassay_005745_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14806_wholemount_strong.wlz

14806_wholemount_moderate.wlz

14806_wholemount_weak.wlz

14806_wholemount_possible.wlz

14806_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14806_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rectum

moderate

regional

moderate expression: see section 12

midgut

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 13 14 moderate expression: see section 11 12

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 12 13 16 20 moderate expression: see section 10 11 14 15 17 18 19 21 22

metanephros

strong

spotted

strong expression: see section 08 09 16 17 18 moderate expression: see section 05 06 07

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7506
Entity Detected:	Hnf4a, hepatic nuclear factor 4, alpha ( MGI:109128)
Sequence:	sense strand is shown >T7506 GGGACCTGGGAGGAGGCAGGAGGAGGGCGGGGACGGGGGGGGCTGGGGCTCAGCCCAGGGGCTTGGGTGG CATCCTGGGCCGGGCAGGACAGGGGGCTAAGGCGTGGGTAGGGGAGAATGCGACTCTCTAAAACCCTTGC CGGCATGGATATGGCCGACTACAGCGCTGCCCTGGACCCAGCCTACACCACCCTGGAGTTTGAAAATGTG CAGGTGTTGACCATGGGCAATGACACGTCCCCATCTGAAGGTGCCAACCTCAATTCATCCAACAGCCTGG GCGTCAGTGCCCTGTGCGCCATCTGTGGCGACCGGGCCACCGGCAAACACTACGGAGCCTCGAGCTGTGA CGGCTGCAAGGGGTTCTTCAGGAGGAGCGTGAGGAAGAACCACATGTACTCCTGCAGGTTTAGCCGACAA TGTGTGGTAGACAAAGATAAGAGGAACCAGTGTCGTTACTGCAGGCTTAAGAAGTGCTTCCGGGCTGGCA TGAAGAAGGAAGCTGTCCAAAATGAGCGGGACCGGATCAGCACGCGGAGGTCAAGCTACGAGGACAGCAG CCTGCCCTCCATCAACGCGCTCCTGCAGGCAGAGGTTCTGTCCCAGCAGATCACCTCTCCCATCTCTGGG ATCAATGGCGACATTCGGGCAAAGAAGATTGCCAACATCACAGACGTGTGTGAGTCTATGAAGGAGCAGC TGCTGGTCCTGGTCGAGTGGGCCAAGTACATCCCGGCCTTCTGCGAACTCCTTCTGGATGACCAGGTGGC GCTGCTCAGGGCCCACGCCGGTGAGCATCTGCTGCTTGGAGCCACCAAGAGGTCCATGGTGTTTAAGGAC GTGCTGCTCCTAGGCAATGACTACATCGTCCCTCGGCACTGTCCAGAGCTAGCGGAGATGAGCCGTGTGT CCATCCGCATCCTCGATGAGCTGGTCCTGCCCTTCCAAGAGCTGCAGATTGATGACAATGAATATGCCTG CCTCAAAGCCATCATCTTCTTTGATCCAGATGCCAAGGGGCTGAGTGACCCGGGCAAGATCAAGCGGCTG CGGTCACAGGTGCAAGTGAGCCTGGAGGATTACATCAACGACCGGCAGTACGACTCTCGGGGCCGCTTTG GAGAGCTGCTGCTGCTGTTGCCCACGCTGCAGAGCATCACCTGGCAGATGATCGAACAGATCCAGTTCAT CAAGCTCTTCGGCATGGCCAAGATTGACAACCTGCTGCAGGAGATGCTTCTCGGAGGGTCTGCCAGTGAT GCACCCCACACCCACCACCCCCTGCACCCTCACCTGATGCAAGAACACATGGGCACCAATGTCATTGTTG CTAACACGATGCCCTCTCACCTCAGCAATGGACAGATGTGTGAGTGGCCCCGACCCAGGGGGCAGGCAGC CACTCCCGAGACTCCACAGCCATCACCACCAAGTGGCTCGGGATCTGAATCCTACAAGCTCCTGCCAGGA GCCATCACCACCATCGTCAAGCCTCCCTCTGCCATTCCCCAGCCAACGATCACCAAGCAAGAAGCCATCT AGCAAGCTGCTGGGGGGGTCAGGGGTTCTGCTGGCTCATACCCTCAGAAGAGCACCTGGGAGTAACCTAG TCATGGCAAAGAAGATGTGACACGAGGGACCAGTCCCAGAGCAGCTACAGAAAGGGTGTGAGGCCCCAAA ACCGTGTGCTGAGGGGCGAATGCGTCGCCACCCCTGACCCCGCATCTGGAGGGCGGGGCTTTGCTTGAGA AGACCCCAGGGAGGACTGTCTTCCACTGCCTGGACTCCTCTCAAGTTGAAGTCATCGTCTTCATCTTCCT CCGTATCTTCTTCCCTCAACTTCTTCACCCCTGAAGGACGACTGTCTGCAGATGTTGCAGGACCTTGTTT TAAAATCAACTCCCTTCTCTCCCAGCGGGGGGCCTCTTCCTTCCTGGTGCTGGTCAGGGGGCCTGGAAAC AAACTTTCTGAGGCTGGGTCAGCTTGCCTTATAGTACTCCTCTCCTGCTATGCCAACCTCAGGTGACCTG CTACCATCCTCTATACCTTCCTCCGCCATCTGACTTCTCTAAAGCCATCTCTCTGGAGGCTGAGGAAGCT GTGGTAACCATTTCCCCACTCATTCTGAGAGCCTTCTGTAAGCACTGACCTGGGTAAGGCAGAGGGTAGG AGTCACCATCCCTCCTCTGCCAATATCGCTACAACTCCTGCCTCCAGCACTAGGCTATAACTTTGGAGTG ATAAGTCCTATGGCTTTCTACAGTAGAGAACCACCCAAGGGTGTCGATTATAACAGAATGTGGAGAGTGG GTGGCCGTCATTGGGAAAGCATCGAGAAAGAGGCGAGCTTTGCATCTGTCCCTTGACAGGGAAGGTAGGC TTCTGGCTAGCAGAGCAGGTGACACAAACCAGTTTATTGGAGAACAGCCTGGACAAAGGCCCATAGGTAG GTAAAATAGTATGGGAGGGAGGCAATGAAGAAATCAGTTCCCATGGAGTTGTGACAGGGTACACACTGGG GACCATTGAATGACAAACTGAGGTCAGGGGTAGCTGCAGTGTGGGGCTGTCAGAAGATGAATACAGCATC CCGCAGAGGTAGGATATGAGGAGGCTATACTGGAGCGTAGGGATGAGATAAAGGGATGGATCCGAGTCCT CCCGGGGCCTGCCAAGCTTTTCCATACTTCCAGATGATGTTATAAGGCATTAAACAGCCACCATCGCATA GAACCTTCCCTGTAACCCAGACATTGTCAAGGAAGATATACGTAGAGGATCTTAACTGACTCACAGGAAG CACTTGTCCCCCCTGGCACGGAATCCAGAGGGTTCCTCGTCCTTTACCAGTGTCAACCCCAGAGGCTTTC TCTCTCCAGAACTGGCCAGCTAGAGACCGTTCTAAAGTCCACTGGAAGTCAAGAATCTCGTTTACTGATA ATAGTAAAGTTGATTCATGACCCCTGTCCTTTTTTTTCTACTGATCTACCCAAGTGTAGGTGAGCCGCAG CAGGGAGTGATTCCTAGAAGACGGATCAGTCTAGTCTGGCAGTCAAGGCTCAGGAGTTTGGGAGAGGGGC AGGAGAAGGATAAGAAAGCCTGGGTTTTAGGCTTGCCTGGGAAGCTACAGTCAAGGTGCATTCTGGGAGC TACTCATCCCCAGCAGTTAGCGCTCCGCCCCGGTTGACTCTTGATGGCTTTGCGGAGCCCCTGCAAAGTG TCACTAGTCAATGGGAACAAGAGGAATGTCCCCTGGTCTGGAAGTGTGCTAAGTGGAAGGAATGGGCTGT GAGACTGGATAGTTATGGTGCATCAAGGCTGATCCTTCTGATTGGGTGGGGCGAGCATCCCAGTGTCCAA AAATTCCAGCCTAAATGACAGGTCCTCTGAAAAAGAGGTGCTGAGGAGTAATGGGGCCAGCTGATTAAAG AAAAAGAACCCCCACACAACAGGACTGTCAGGACAGATTGAGTTAACCCCTCAAGCCAAGTCCCGGGTGT CAGGAACAGTTGTCCATGCTCAGAGCCCTCAAGCCATCCTTGGCCACTCTCACTCCAGTGCCCCCCAGAG ACCCCAGCCAGATGGCTCAGACTGTCCTGCATGAAGGCAGGAGAGCTGAACTCTCTCTGGTCCTTGTCTT TCCCAATCTTTGTCCCTCCATCCCCAGTGGCATCAGGAGAGGAGTAGGTAATGTTGGCCTTCAGTGGTAG CAGTCTTCTGGGGCAGGGGTCAGCCACTGTCCCAATCAGTCCTTTCTGAAGGAGAAACCCATTCCACATT AACAAATTTATTACAGTACAGGGAACATGGAATAAAGGAAAGTTTACTCTTCTGATTATAAGCTGAGGAT GCTATGTTGCAGTTTGTTTGCTTGTTTTTTTCTTCTGAGCTTTGTTTCTAGTACACGATATGACGGGACC AAGTACAAGCTGGTGGTGGACAGAGCTGGGTGCCACAGTTTTCCACCAAGAGCAGCCTCTTTTCTTCATG GAGTTCTCAGAACCTTTCAGGGTTCAGGAGGCTCCCCTGAGAATAGACAGGAGAGGGTCAGAAGCAGACG TCCTCCTTTTCTTGTGATAACCACGCTACTTGCCTTTGCTAGAGCTCTCTGTGGCCAACCCTGGGAAATG CTAATCATGACCTTCACACCAAACATTCAGAAGTAGGCTGTGTCCCATCAGATCAATCTGCACCTTCCTG TGGTTGTGCAAACATCACGGGGCCCTACCCTGATCCCCAAGGCCCCACCATCACTGCCCAAGGGGGTAAA AAAGAGAAAAAGCCCTCAAAAACAGTTCTTACTCCTGTGTTTTAGCAAAAATTTATCAAACAAAATAAAT GGTACTTATGTTTTCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4238842 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4238842 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14805 same embryo
	EMAGE:14804 same embryo
	EMAGE:14802 same embryo
	EMAGE:14807 same embryo
	EMAGE:14803 same embryo
	EurExpress:euxassay_005745 same experiment
	MGI:4825402 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source