EMAGE mouse gene expression spatial database

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EMAGE:14815

Slc4a10 solute carrier family 4, sodium bicarbonate cotransporter-like, member 10 ( MGI:2150150)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005787. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005787_01	euxassay_005787_02	euxassay_005787_03	euxassay_005787_04

euxassay_005787_05	euxassay_005787_06	euxassay_005787_07	euxassay_005787_08	euxassay_005787_09

euxassay_005787_10	euxassay_005787_11	euxassay_005787_12	euxassay_005787_13	euxassay_005787_14

euxassay_005787_15	euxassay_005787_16	euxassay_005787_17	euxassay_005787_18	euxassay_005787_19

euxassay_005787_20	euxassay_005787_21	euxassay_005787_22	euxassay_005787_23	euxassay_005787_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14815_wholemount_strong.wlz

14815_wholemount_moderate.wlz

14815_wholemount_weak.wlz

14815_wholemount_possible.wlz

14815_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14815_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 09 10 11 12 13 14 15

olfactory lobe

weak

regional

weak expression: see section 13 17 18

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4127
Entity Detected:	Slc4a10, solute carrier family 4, sodium bicarbonate cotransporter-like, member 10 ( MGI:2150150)
Sequence:	sense strand is shown >T4127 AGCAGAGCGAGTGCCCGGCTGAGTGGAAGACACTGAAGACACTGCAGAGCAAGGTGCTTTTTTTCCAGAG GTGTTACAGAACATGGAGATTAAAGACCAGGGAGCCCAAATGGAGCCGCTGCTGCCTACGAGAAATGATG AAGAAGCCGTTGTGGATAGAGGTGGAACACGCTCTATTCTCAAAACACATTTTGAGAAAGAAGATTTAGA AGGTCATCGGACATTATTTATTGGAGTTCATGTGCCCCTGGGTGGAAGAAAAAGCCATCGTCGTCACAGG CATCGTGGTCATAAGCACAGAAAGAGGGACAGAGAGAGAGATTCGGGACTGGAGGATGGAAGAGAGTCCC CTTCTTTTGACACCCCATCGCAGAGGGTGCAGTTTATTCTTGGAACTGAGGACGATGATGAGGAGCACCT CCCTCATGACCTTTTCACAGAGCTGGATGAGATTTGCTGGCGTGAAGGGGAAGATGCTGAGTGGCGAGAG ACAGCCAGGTGGTTGAAATTTGAAGAGGATGTGGAAGATGGAGGAGAAAGATGGAGTAAGCCCTATGTGG CCACGCTTTCATTACACAGCTTGTTTGAGTTGAGAAGCTGCATCCTGAATGGAACTGTGCTACTGGACAT GCATGCCAACACGATAGAAGAAATTGCAGATATGGTCCTTGACCAGCAGGTCAGCTCAGGCCAGCTGAAT GAAGATGTTCGCCACAGGGTCCACGAAGCATTGATGAAGCAGCATCATCACCAGAATCAGAAAAAACTGG CTAACAGGATTCCTATTGTCCGATCTTTTGCTGATATTGGCAAGAAACAATCAGAACCAAATTCCATGGA TAAAAATGGTCAGGTTGTTTCTCCTCAGTCTGCTCCAGCCTGTGCTGAGAATAAAAATGATGTCAGCAGG GAAAACAGCACTGTAGACTTCAGCAAGGTTGATCTGCATTTTATGAAAAAGATTCCTCCGGGTGCTGAAG CTTCAAACATCTTGGTAGGAGAACTGGAGTTCCTAGACAGAACTGTGGTTGCCTTTGTCAGGTTGTCTCC AGCTGTCTTGCTCCAAGGACTTGCTGAAGTTCCAATCCCAAGCAGATTTCTGTTCATCCTTCTGGGACCC CTGGGAAAGGGTCAACAGTACCACGAGATTGGCAGATCGATTGCGACCTTAATGACTGATGAGGTGTTTC ATGATGTTGCTTACAAAGCTAAAGACCGCAATGACTTGGTATCAGGAATTGATGAGTTTCTGGATCAGGT TACCGTTCTTCCTCCTGGAGAATGGGATCCAAGCATACGAATAGAACCTCCCAAAAATGTCCCTTCCCAG GAGAAGAGGAAGATTCCTGCTGTACCAAATGGAACAGCAGCTCATGGCGAAGCTGAGCCACATGGAGGAC ACAGCGGACCTGAACTCCAGCGAACTGGGAGGATTTTTGGGGGACTTATATTAGATATCAAAAGAAAGGC TCCATTCTTCTGGAGTGACTTCAGGGATGCTTTCAGCCTGCAGTGCTTAGCATCGTTCCTGTTTCTCTAC TGTGCATGCATGTCTCCTGTCATCACATTTGGAGGACTGTTGGGAGAAGCAACTGAAGGTCGTATAAGTG CAATCGAATCACTCTTTGGAGCATCTATGACCGGGATAGCCTATTCTCTTTTTGGTGGACAGCCCCTGAC CATATTAGGCAGCACAGGACCTGTTTTGGTGTTTGAAAAGATCTTGTTTAAGTTTTGCAAGGAATACGGC CTGTCGTACTTGTCCTTACGGGCCAGCATTGGGCTCTGGACTGCAACACTGTGCATCATCCTTGTGGCCA CGGACGCGAGCTCACTCGTCTGCTACATCACCCGGTTTACCGAAGAGGCTTTTGCTTCTCTCATTTGCAT CATTTTTATCTATGAAGCCCTGGAGAAGTTGTTTGAGCTCAGTGAAACCTATCCAATCAATATGCACAAT GATTTGGAACTGCTGACACAATACTCATGTAACTGTATGGAGCCACATAGTCCCAGCAATGACACACTGA AGGAATGGCGGGAGTCCAACCTTTCTGCCTCTGACATAATCTGGGGGAACCTAACTGTGTCAGAGTGCAG ATCACTGCACGGGGAGTATGTCGGGCGAGCCTGTGGCCATGGCCACCCCTACGTGCCAGATGTTCTCTTC TGGTCGGTGATCCTGTTCTTCTCCACAGTTACCATGTCAGCCACCCTGAAGCAGTTCAAGACCAGCCGCT ATTTCCCAACCAAGGTTCGATCCATAGTGAGTGATTTTGCGGTTTTTCTTACAATTCTGTGTATGGTTTT AATTGACTATGCCATTGGGATCCCATCACCAAAACTACAAGTACCAAGCGTTTTCAAGCCGACCAGAGAC GACCGTGGCTGGTTTGTTACACCTTTGGGTCCAAACCCATGGTGGACAATCATAGCTGCCATCATCCCAG CTTTACTCTGTACTATTCTGATTTTCATGGACCAGCAGATTACAGCTGTCATCATCAACAGAAAAGAGCA CAAGCTAAAGAAAGGTTGTGGCTATCACCTGGATCTGTTAATGGTGGCAGTCATGCTCGGGGTCTGCTCC ATTATGGGCCTGCCATGGTTTGTGGCTGCCACAGTTCTCTCCATCACTCATGTCAACAGCCTCAAGCTCG AATCAGAGTGCTCTGCTCCAGGAGAACAACCCAAGTTTCTCGGCATTCGGGAGCAGAGGGTGACCGGGCT CATGATTTTTATTCTTATGGGTTCATCCGTTTTCATGACCAGCATTCTGAAGTTTATCCCCATGCCAGTG TTATACGGAGTGTTTCTTTATATGGGTGCTTCGTCTCTCAAAGGAATTCAGTTATTTGATAGAATAAAGC TCTTCTGGATGCCAGCCAAACATCAACCAGATTTCATCTATCTAAGGCACGTGCCCCTCCGGAAAGTCCA TCTCTTCACAGTCATTCAGATGAGTTGTCTCGGCCTTCTGTGGATAATCAAAGTTTCGAGAGCTGCTATT GTCTTTCCTATGATGGTGTTGGCACTAGTGTTTGTGAGAAAGTTGATGGACTTCTTGTTTACCAAACGGG AACTCAGCTGGCTTGATGATTTAATGCCTGAGAGTAAAAAGAAGAAACTTGAAGATGCTGAGAAAGAAGA AGAACAAAGTATGCTAGCCATGGAGGACGAGGGCACAGTACAACTCCCACTGGAGGGACACTACAGAGAC GACCCGTCTGTGATCAATATTTCTGATGAAATGTCAAAGACTGCCATGTGGGGGAACCTTCTAGTTACTG CTGACAACTCAAAAGAAAAGGAGTCACGCTTTCCTTCTAAAAGCTCCCCTTCCTAATCACTCTAGAAGCT GATTCCCCAAAGCAATGAAAGCCGAAAGGAGAAGAAAGCTGACTCAGGGAAAGGCGTTGACAGGGAGACT TGTCTATGACTTGATCTTCAATTTATTTTTTACATATATATATATGAGAAGAGTGTCACAATTATTAACA AAACTGCTTTGATCATGTAATTGTAAACCCTCTCTCCCATCCCACCTTCATACTGTAAGTAGTGCAAGCC TTCATTCTATTTCTGTGTTCAGCCTCTGAGCAGGTCGACACCCTTGTAAGCAGATCCAATAGCTAATGCA AGAGTCTCCAGTGTTACTGCCGTAAGACATTCGCCAACACAGGATTCTCATTGTTGACATTAAGAGAACA AAGCTTTCTTTAAAAGATAAGTCATATTTGCCTAGTTTGTATTTTCCTACCTTAGTAACCTGAAGATGCC TGATAATTTTATTCAGAAGAATTTTGAAAGGTAGTCGTACTTTTTATTTTTTATGGCTTAGCATTCGTTA CTGGTTTTGAAAGACCCAAATCAAAAAGTTACTCTGAAAGCATTTTTAATAATTGTATTTATGTATTTCC TTGACTTAATATGAAACATTTAATACTTAATAACTGTTACTTCAAGTCATTTGAGAAAGAGACCTGTTCA TATCTTCTTAAAAGACATACTGCAAAGAGTCAAGTAGTGTTCACTTAGAATTCAAGTTGTAACCATGCAG TCAAAAACTAGGCTTGTATTAAATGCTTTAGAGATATTTGAAGAGTTTTGTGGGGCTTTTCATTTTAAAT CTTTACCAGAAATATGCTACTGAGTTTCTCTCCCATTGACAAGGGTTGCTTCCCGAATAAGCCTATGACA TACATACTTACGGAATGCCACATGGTGCAACATTGTACATTTGATGCCAGCCCTGGCAGCTGTTCTGCTG ACCATGGTCATGTGCTGCTAAGTTTGGTTCCTATCATGTTGTCATGTTAGACCAACAGGTCTCCAACTGT ATTTTGTTTTTTTTGCAAAGCTCTTTTCCACATTTTAACTAAATGCATGTTGTGGAAAAATAGTCTTTGA AATAAAATTTCAGATTTTGTTAGAAAAGGTTATGTAAATACTTCAGTCCATATGAAACAGTTCAACTTTA TTGAAACAGGAAGGAGATTATGGATTTTTGAGTATTACTAAATATAAATTTCATTTAATTTTCAATAAAT GTGCTTTAATACAAAACAAAATATCATAGGGGTCTTAGTTCCTAAAAAAGTATCAATGATTAACAACCTT ATAATCTTTCAATGTCCAGGTTTAGAAAAATTCAGAGCCTTCTGGGTTTTATAAATTACATGTACTCTGT GTAAATACACATAATTAGAAAAATCCTCTTTGCTTTTAAGCTAATGAAGACGAGAGACAACAGAGCCTAC ATAACCTTAATATTCTGATATCTTTGAACAAAAAATTTCCTCAGAATCCTTTCAGGAGCCATTTTTTTAA TGAGATATGAGCCAAAATTGTGAGAAGAATTTTCAGTTCGTAAAGTCTGTATTTATAAATGGTAAAGAAA AATGCAAAAGTGTCTTTTCCAAATGTGCTACCTTTGTGTATGTTGTAATAGCGACACTCTCTCTAAACAT TCTCGCTGTCTATGACTTAGCAGGCCAATCCCCAAAGCACTCTCCTGGTGTCTCTAGAGTGTGATGTCTG TTCTGTTGAAATGACCAGTGAGTGACACTTCACATGATCACTGGTTTAAACAGGCAATCAGCCTATGAAA TTCTGTATTTCTGAATATTTTTATAGTAATTTTGTTCTTGTGTGAATTTTAATGCTATCTCTATCTTAAT CTTAATATTTTGAAATCACATAAAATATAAGAAAATGTAGTATTCTATATTTACTCCTAATTTCAGATTC CTGGTCAAAATTACTGAATATCTTGAATGTAATTTATTGCAATGTTTAAGTACTGTGTAAATGTGACAGG ATATTGTGTTTTTCAAAACTAAGAAATGTTATGTGGAAATAAATATTTATCCTACCTAAAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:4480841 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4480841 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14816 same embryo
	EMAGE:14817 same embryo
	EMAGE:14814 same embryo
	EMAGE:14819 same embryo
	EMAGE:14818 same embryo
	EurExpress:euxassay_005787 same experiment
	MGI:4828241 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source