EMAGE mouse gene expression spatial database

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EMAGE:14818

Pex26 peroxisomal biogenesis factor 26 ( MGI:1921293)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005795. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005795_01	euxassay_005795_02	euxassay_005795_03	euxassay_005795_04

euxassay_005795_05	euxassay_005795_06	euxassay_005795_07	euxassay_005795_08	euxassay_005795_09

euxassay_005795_10	euxassay_005795_11	euxassay_005795_12	euxassay_005795_13	euxassay_005795_14

euxassay_005795_15	euxassay_005795_16	euxassay_005795_17	euxassay_005795_18	euxassay_005795_19

euxassay_005795_20	euxassay_005795_21	euxassay_005795_22	euxassay_005795_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14818_wholemount_strong.wlz

14818_wholemount_moderate.wlz

14818_wholemount_weak.wlz

14818_wholemount_possible.wlz

14818_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14818_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9017
Entity Detected:	Pex26, peroxisomal biogenesis factor 26 ( MGI:1921293)
Sequence:	sense strand is shown >T9017 GGGTTCCCCGTGCAGGTCTTCTGTTGTCGCGCTCTCTACTCTGGGAGTTTCGGTGCTTTTAGCACCGGTC CAGGGACAGGAGGAACTAGTGCTGGGCCTTCGGCAGCACTAGTCTTGAACAGACAGTCTTGAACAGGCCA GTGCCCCAAGGGTTAGGCTGTCAGTGAGCGCAGGCTGTGCCCGGGTCCAGCTCCTTCCTGGTCCTTCCTT AGGAACAGACCTGGAGAGGCGCGGAGGGGAAGAGTGCGAGGCTTGCGGGACATCTACCAGGCCCAGTCCG AATTTCCAGGAAGCTCCCGAGAAGCTGTCCTCCTGACGTGTGAGGAGACCTGGTCGAACCCAGGCTGGCT ATGAAGAGCGACGCCTCGACGTCTGCAGCCCCTTTGAAAGGGCTCGTGGGCCCTCTGCGGAGCAGCGAGC CCGCGCTCGCCCTCCCCGCTGTGTCCCCCGCGGTGCACCTGCTGGAAGAGGCGTCCGACCTGTTGGTGGT GCACCTGGACTTCCATGCCGCACTAGAGACTTGCGAACGAGCCTGGCAGAGCCTGGCGGAGGAGCCCGTG AGTGGCACCATTGTGGAGGTGAAATGCTCCCTGTGTGTGGTGGGGATCCAGGCTCTGGCAGAAATGGATC GATGGAGAGAAGCCCTGTCCTGGGTCCTCCGGTACTACCAGGTTCCTGAAAAGCTGCCTCCCAAAGTCCT GGAGCTGTGCATCCTTTTATACAGCAAAATGAAAGAGCCCGGAGCTGTGCTGGATGTGGCCAGTGCCTGG CTCCAAGATCCAGATAACCAGGGCCTTCCTGATTATGGATCGCTGGCCAGACTCCACGTGTTCCGGCTGC TGCTACCCTCGGGTCGCTTGTCAGAGGCCGAGGAACTGGCAGTGCGCTCTGCGGCCTTCAGCGAGGAGCA GCGGGTAGAAGCGCTCCAGGCCATCCACTTAGCACGGCAGCAGCACACGCAAGAGCACACGCAGGAGCAC TCGGACTCCCAGGAGCCCCAGAAGCTGAGGCAGGAAGGTTCGTTCTCCCAGAAGTTGCTGTCGCTGCTGA TGCTGCTTCGCCGGCTCTGGGGCTCCGTGGTGAGCCACCTCCTCTCGCAGCCCTTCAGAAAGGGCCTCCT GGCTGCCTTGATCCTCTGCCTCTTGATTCTACGGTTTGACCCAGCAGCTCCTTCTTCGCTGCCCTTCCTC TACCAGCTGACCCAGCTCTTCCGCCGGATCCAGAAGGCCACACTTTCTCGGCTCTACCCCCTCGCCCTCC GGGACTGAGTGGTCCTACCTGGCTGTCTCTACCTCTTACACTCCACATCTATAGAAGCGGAGAAGTTGAG CCCAGTGCCTGGGTCCAAGATGGCACCTTGGGAATGGGCCCATTGGTTCTAGAAGGAGTGACCAAGGCAG GTGAGCCATGGATGGCTGACTTTGAACCCTCCCTTTGGTATCCCACTGTGGCTGGCCTCAGAGGGAGTTT GGGAACAGTGGTGCCCGAGGCCCAGTGCACAGAACACAAGTCTGGCTGCCCAGAACACTGCAACAGCTCA TCAAAAAGGAGGGTTAGGAGCAGGGAGTGGGGCTTGAGACAGTCTCATTTCTTGACAAAGGCAGAGGGTG ACTTTCCAGATCTGGTGCTGAGGGATCTGGGATTTACAGATTTACCTCTGAAAACTGCTCTACAGCTCAG AATTTGAATTTGAGTAAGACCAGAGGTCCCTCGGTCCCCCACTCCCCTCCTCCATACCCTTCCCCCACGC CCCTCCCACAGATCAGTTCACCATCCCCTCCCATTGGTCTGGCAGTAGGGCAAGAGGCTTTCTGCTAGAG GCCTTTTGCTGTTCAGACCGGATATGGCTTCTGACTCACCCAGGTGGCCACCTGGAGCAAAGTGAGCCTG CCTGACCTTATCTCTGGGGAATGTGATCTTTCTTGGTGCCGCTGAGTCTTAGCTCAGTTTTCCAGCTCGC CAGGGGCCAAGCTCTGCCCTCTGGGACCCAGTGTCTTCCCACCACACCCCACCCCACCACCTTTTGATCT GTCTAGCCAACTGTGTCACACCACTGTCTCTCCTTGATCCTTTCTCTGGGTCCTAGTTGTGGTCCATTGT TTGAATAATGCCCTAGTCTCTCTGCTCGTGTGGTGTGAAAAGTAGGCCAGGCTCAGGGCTGACAGCTGAA AATGGAACTGGGTAATCAGGTGAGCTGTGAGAACCGTGGGGGCTGGGCAGAGGGGCTGGGGGGTGGGGCA AAGGGACAACCCCAGGGAGGAGGCACTGCGTGCGTGCGCTTCAGCCTCAGCATTTCTGTGTAAAAAAATA CAGGCTCCTCTGGTGCCGCCAGGAAGGAGGTCCTCAGCCGCCCACCCACACTGGATCCCTGACACGGGAT GGCAAGAGTGCAGAGGCTTTGCTAGTCTTTTGTTGGCTGATATTTAGGAACTGTGAGTCAACAGCTTTTC TCTAGAAGAGGAAACGTCACAGAAACTTACTTAGAATTCCATCTAAACCCTGAAGACTTGGCAACTAGGT TCATCATTTTTTTTTTTTTTTTGTATGCCTTTTAAGTAGACCCTAGATCACACTGGGGAGGGAAAAGGAT TCAGGAAGGGTTGAAATCCCTTGACAGTGATTCCTTCAGTGTTGTCGCCCAACTTGGCCCAGAACACCTG GGCTCCAGTGCTGAGCTCCGCCCTCCAGCTTCGAGCGTCTGTGACTCACACACCTTATGACAGTGGTTTT GAAGTGAGGACCTACCCATTCACTGCCCTAGAAGAAACTCCTTAACGGCTTTCATGGCCCACAGGATACA AATCCAGATTCTTTTTAATAGAAACTTAGGCCAGTGTTCGCACGTCTGGCTGACAGCTCCTTTACTGTGT TGCCCTGTTCGCTGAGCAGGCTGTACTCTAGCTGCCAGGTCCTCCTCAGGCCTCCCTGTGATGGCTTAGC TGTATCTGCTGGAGGGTCTTGGAGCAGCTCTGCTCTAGACCCTTGGCTTTCCAGGCTCTCAAGGGTTACC CAGCATTCTTTGCTGCCCAGCCCATCTCCTGCCCCTCCTGTAGTGGGGGCCTGGTGGTGGGTGGTGGGAC TGTTTGCCTCTTCCAGTGCCGTAAGCTCCATGAGGCTGCAGAGTGTTTGAGTCGGGCTGGCATTCCTGTG TGGGTGACTGTTGGCCAGGGTTCTGCTTTCTCATCTGTAAGTCGGTTCTCTTCTCACAGGGCCTCTTCTA CGAGTAATGGGCACTTAGGACTCTTGGCACTTTTGACAGCCACTAGGAAGAGTTCTCGTGAGCAGTGGTA TTCCCAGGCTCAGCTGAGCATGGGTGGGTCCTCAGGGCTGAGTGGACTGGTGGAAGAGGGCATGCGCTTC TGGTGCTGTGAAACCCTGAACAGATCGGCTTGTCTTTCTGCTCTCTCCTGATGCTCCTCACAGTTGTTCT GCTGGTGGCCCATGGGGCTCTGTTTATTTTTACCTGACTGCTGCAGGGCTGTCTGTACCTTAACCACCTG CCTATCCAGCCAGGAACTCGAATCCCAAACTGTTGCTGTTACTTTGTCCTTTCCAAGGATGTAGGCTGGT CCTCGGCTCTGGGCTTAAAGAGGTCAGAGTGAAGGGAGGGCCTTCTTGGTGGGTCCAGCACAGCAGCCAT CAGCCAAAGGTAGCTTTTGAGCATTTGAATTGTGTGCGGCTCATCCACGTGGACACATGCTGTAATTGTT AAACATGCACACTGGATTTCAAAGACCAAGTACTAAAAAATAAAATAAAAGAATGGAAACACAAAAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6811976 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6811976 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14816 same embryo
	EMAGE:14817 same embryo
	EMAGE:14815 same embryo
	EMAGE:14814 same embryo
	EMAGE:14819 same embryo
	EurExpress:euxassay_005795 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source