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EMAGE:14982

Dync1h1 dynein cytoplasmic 1 heavy chain 1 ( MGI:103147)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14982 EMAGE:14982 EMAGE:14982 EMAGE:14982 EMAGE:14982
"Pseudo-wholemount" of euxassay_008019. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008019_01 euxassay_008019_02 euxassay_008019_03 euxassay_008019_04
EMAGE:14982 EMAGE:14982 EMAGE:14982 EMAGE:14982 EMAGE:14982
euxassay_008019_05 euxassay_008019_06 euxassay_008019_07 euxassay_008019_08 euxassay_008019_09
EMAGE:14982 EMAGE:14982 EMAGE:14982 EMAGE:14982 EMAGE:14982
euxassay_008019_10 euxassay_008019_11 euxassay_008019_12 euxassay_008019_13 euxassay_008019_14
EMAGE:14982 EMAGE:14982 EMAGE:14982 EMAGE:14982 EMAGE:14982
euxassay_008019_15 euxassay_008019_16 euxassay_008019_17 euxassay_008019_18 euxassay_008019_19
EMAGE:14982 EMAGE:14982 EMAGE:14982 EMAGE:14982 EMAGE:14982
euxassay_008019_20 euxassay_008019_21 euxassay_008019_22 euxassay_008019_23 euxassay_008019_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14982Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14982_wholemount_strong.wlz
14982_wholemount_moderate.wlz
14982_wholemount_weak.wlz
14982_wholemount_possible.wlz
14982_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14982_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forebrain
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
hindbrain
moderate moderate
homogeneousmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
midbrain
moderate moderate
homogeneousmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
facial vii ganglion
strong strong
regionalstrong expression: see section 04 05 19 20
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 06 17 moderate expression: see section 16
trigeminal v ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22
vagus x ganglion
strong strong
regionalstrong expression: see section 07
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 06 07 17 moderate expression: see section 18
trigeminal v nerve
strong strong
regionalstrong expression: see section 17 moderate expression: see section 10
spinal cord
moderate moderate
homogeneousmoderate expression: see section 06 07 08 09 10 11 12 13
dorsal root ganglion
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 13 14 15
neural retina
moderate moderate
regionalmoderate expression: see section 02 03 04 05 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36296
Entity Detected:Dync1h1, dynein cytoplasmic 1 heavy chain 1 ( MGI:103147)
Sequence:sense strand is shown

>T36296
ACATTTAGCAGCATCCCTGTCTCTAGGATATGCAAGTCTCCAAATGAGCGCGCCCGCTTGTATTTCCTGC
TGGCTTGGTTCCATGCCATCATCCAAGAGCGCCTGCGCTATGCTCCACTGGGGTGGTCAAAGAAGTACGA
ATTTGGAGAATCTGACCTTCGATCAGCTTGTGACACGGTGGACACGTGGCTGGACGACACAGCCAAGGGC
CGGCAGAACATCTCACCGGATAAGATCCCATGGTCTGCCCTGAAGACCCTGATGGCGCAGTCCATCTATG
GTGGGCGGGTGGACAATGAGTTCGACCAGCGTCTGCTCAACACCTTCCTGGAGCGGCTGTTCACCACCCG
CAGCTTCGACAGTGAATTCAAGCTGGCGTGCAAGGTTGACGGGCACAAGGACATCCAGATGCCTGATGGT
ATCAGGCGAGAGGAGTTTGTGCAGTGGGTGGAGCTGCTGCCTGACGCCCAGACACCCTCATGGCTGGGCC
TCCCCAACAATGCTGAAAGGGTCCTGCTTACCACCCAAGGCGTGGACATGATCAGCAAGATGCTGAAGAT
GCAGATGCTGGAGGACGAGGATGACCTGGCCTACGCAGAGACGGAGAAGAAGGCAAGGACGGACTCCACC
TCCGATGGCCGTCCAGCCTGGATGAGGACACTGCACACCACGGCGTCCAACTGGCTGCACCTCATCCCAC
AGACACTGAGCCCCCTGAAGCGCACCGTGGAGAACATCAAGGATCCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91739. Forward Primer - name:091739_F_cDNA_Dnchc1, sequence:ACATTTAGCAGCATCCCTGTCT; Reverse Primer - name:091739_N_SP6_cDNA_Dnchc1, sequence:AGGATCCTTGATGTTCTCCAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14979 same embryo
 EMAGE:14980 same embryo
 EMAGE:14978 same embryo
 EMAGE:14983 same embryo
 EMAGE:14981 same embryo
 EurExpress:euxassay_008019 same experiment
 MGI:4824427 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS