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EMAGE:15146

C230081A13Rik RIKEN cDNA C230081A13 gene ( MGI:2442366)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15146 EMAGE:15146 EMAGE:15146 EMAGE:15146 EMAGE:15146
"Pseudo-wholemount" of euxassay_009542. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009542_01 euxassay_009542_02 euxassay_009542_03 euxassay_009542_04
EMAGE:15146 EMAGE:15146 EMAGE:15146 EMAGE:15146 EMAGE:15146
euxassay_009542_05 euxassay_009542_06 euxassay_009542_07 euxassay_009542_08 euxassay_009542_09
EMAGE:15146 EMAGE:15146 EMAGE:15146 EMAGE:15146 EMAGE:15146
euxassay_009542_10 euxassay_009542_11 euxassay_009542_12 euxassay_009542_13 euxassay_009542_14
EMAGE:15146 EMAGE:15146 EMAGE:15146 EMAGE:15146 EMAGE:15146
euxassay_009542_15 euxassay_009542_16 euxassay_009542_17 euxassay_009542_18 euxassay_009542_19
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euxassay_009542_20 euxassay_009542_21 euxassay_009542_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15146Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15146_wholemount_strong.wlz
15146_wholemount_moderate.wlz
15146_wholemount_weak.wlz
15146_wholemount_possible.wlz
15146_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15146_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37601
Entity Detected:C230081A13Rik, RIKEN cDNA C230081A13 gene ( MGI:2442366)
Sequence:sense strand is shown

>T37601
AGTCCACGACAAAAGATCCCTAAAATAGAATTAAGTACTGGGGGACCTGGGCCAAATGTCCCTCCAAGGA
GAAACTGCCATAAGTCGGCGCCCACTTCACCCACGGCTACAAACATCTCCTCTAAAACCATCCCTGTTAA
GTCACCTAATTTGTCTGAAATAAAATTTAATAGTTACAACAATGCCGGGATGCCACCTTTTCCCATTATC
ATCCATGATGAGCCATCCTATGCCCGGAGTTCCAAAAATGCCATTAAAGTCCCCATTGTTATTAATCCAA
ATGCGTATGACAATCTCGCCATCTACAAAAGTTTTCTGGGAACAAGTGGAGAACTCTCTGTGAAGGAAAA
AACCACAAGCGTAATAAGCCATACTTACGAAGAAATAGAAACTGAAAGCAAAGTGTCTGATAGCACCCCC
AGCAAGCTCACAGACTGTCCCCAAGCTAAAGGGTTTTCCAACAGCACAGAGCGGAAAAGAGGCTCAGTAG
CGCAGAAGGTGCAGGAGTTTAACAATTGTCTCAACAGAGGTCAGTCTTCTCCACAAAGAAGCTACAGTTC
CACCCACAGCTCCCCAGCAAAAATCCAGAGACCCACTCAAGAGCCTGCGGGCAAAACGGAAGGGGCACAA
GGGTCTCAGGTGCCAGGCAGCAGCAGCAACAGCACTAGGGAGAAAGCAAGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 67767. Forward Primer - name:067767_F_cDNA_C230081A13Rik, sequence:AGTCCACGACAAAAGATCCCTA; Reverse Primer - name:067767_N_SP6_cDNA_C230081A13Rik, sequence:GCTTGCTTTCTCCCTAGTGCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15148 same embryo
 EMAGE:15149 same embryo
 EMAGE:15145 same embryo
 EMAGE:15150 same embryo
 EMAGE:15147 same embryo
 EurExpress:euxassay_009542 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS