EMAGE mouse gene expression spatial database

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EMAGE:15197

Ldlrap1 low density lipoprotein receptor adaptor protein 1 ( MGI:2140175)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010445. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010445_01	euxassay_010445_02	euxassay_010445_03	euxassay_010445_04

euxassay_010445_05	euxassay_010445_06	euxassay_010445_07	euxassay_010445_08	euxassay_010445_09

euxassay_010445_10	euxassay_010445_11	euxassay_010445_12	euxassay_010445_13	euxassay_010445_14

euxassay_010445_15	euxassay_010445_16	euxassay_010445_17	euxassay_010445_18	euxassay_010445_19

euxassay_010445_20	euxassay_010445_21	euxassay_010445_22	euxassay_010445_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15197_wholemount_strong.wlz

15197_wholemount_moderate.wlz

15197_wholemount_weak.wlz

15197_wholemount_possible.wlz

15197_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15197_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

naris

weak

regional

weak expression: see section 14

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 11 12 13 15 16 17 18 19

nasal cavity respiratory epithelium

weak

regional

weak expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30226
Entity Detected:	Ldlrap1, low density lipoprotein receptor adaptor protein 1 ( MGI:2140175)
Sequence:	sense strand is shown >T30226 AGCTGGGCCTGCTTTTCTGTTTTGTCTGTTTGTCCTTGTGTGTGCTCCATTTTCTGCTTCCCCACAATTC CTGGGATGATACCAAAGCCTAGCACTAGGCTGTGGCAGGCGATTCTTGCATCCATTTTACAGATAAAGAA ACTGAGGCGACACACATGTTGAGGGGTGGAGTTGAGAACAGAACCCAGATCTGATACCAAAGCTGCCGTC TGTGCTGCCACCTACCAGCCCACGCCTCTTTCTCTGTGGCTTTGTTGTCCTCCCAGGAACCAAAACTCCC CAGCTATTTTCTGACCAAAATGTGTTTCATAACAAACCATCTGGTGCCTTTTCACACAGCGCTGGCGCAG GCCGCCATGCCCTGCTAGCTGCTATGCCGCTGACTTCTGGGCAGATGTGTTTTGAAGTCTGTTAATTCCA TGGGTGAAACGGTATCTCACTCTAGAAACATCTTGCTGTCCTTTTCTGACCTGCAGCCGTAGTGGTCCCT CGACCCTAGCTGGTGGTCGGGGCGGTCCAGTGGTGAGCTTCTCTTAATCTCTTTTCTCCCTTCGCTGGTC CCGTGGGCTCTGGCATCCTGAATCTCAGCCAAACTAACAACATCTTTCCATCGCTTGTGAAAGCTGGTCT CAGTGTCCCCAGGAAAACACACTTTTCCTGAGTGGCTGAGTCAGACCCTTGCCTTGGGGGTTGGGGGGCA CTTGGAGAATTCTTGCTAGAATAAAGGTTGCCAGCTCCCTTTCCCTGGCCAGCTCCAAGGCCCCTGCTCC TTAGCCCAAGTGGCCAGGGTCCGGGCGCGAGGGGGACTGTGTGGTGTTGGGTGATG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5067154), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 24557. Forward Primer - name:024557_F_IRAV59-62_A05_AA691260, sequence:AGCTGGGCCTGCTTTTCT; Reverse Primer - name:024557_R_SP6_IRAV59-62_A05_AA691260, sequence:GCATCACCCAACACCACA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15198 same embryo
	EMAGE:15195 same embryo
	EMAGE:15194 same embryo
	EMAGE:15193 same embryo
	EMAGE:15196 same embryo
	EurExpress:euxassay_010445 same experiment
	MGI:4825894 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source