EMAGE mouse gene expression spatial database

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EMAGE:15202

Gnao1 guanine nucleotide binding protein, alpha O ( MGI:95775)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010416. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010416_01	euxassay_010416_02	euxassay_010416_03	euxassay_010416_04

euxassay_010416_05	euxassay_010416_06	euxassay_010416_07	euxassay_010416_08	euxassay_010416_09

euxassay_010416_10	euxassay_010416_11	euxassay_010416_12	euxassay_010416_13	euxassay_010416_14

euxassay_010416_15	euxassay_010416_16	euxassay_010416_17	euxassay_010416_18	euxassay_010416_19

euxassay_010416_20	euxassay_010416_21	euxassay_010416_22	euxassay_010416_23	euxassay_010416_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15202_wholemount_strong.wlz

15202_wholemount_moderate.wlz

15202_wholemount_weak.wlz

15202_wholemount_possible.wlz

15202_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15202_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 09 10 11 12 13

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

pituitary gland

moderate

regional

moderate expression: see section 10 11 12 13 14 15

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 18 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 16 17

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 07 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 16 17 18

trigeminal v nerve

strong

regional

strong expression: see section 09 10 17

spinal cord

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14

cervico-thoracic ganglion

strong

regional

strong expression: see section 08 14

cervical ganglion

strong

regional

strong expression: see section 08 15

thoracic ganglion

strong

regional

strong expression: see section 10 11 12 13

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 11 13 14 15 16

neural retina

strong

regional

strong expression: see section 02 03 04 05 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20

vomeronasal organ

moderate

regional

moderate expression: see section 16

heart atrium

weak

regional

weak expression: see section 10 11 12 13 14 15 16

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07

midgut

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30558
Entity Detected:	Gnao1, guanine nucleotide binding protein, alpha O ( MGI:95775)
Sequence:	sense strand is shown >T30558 TCGTGCCAGCCTAGTCGTGCGGGCTGTGGCAGGGAAGGGGCCACCATGGGATGTACGCTGAGCGCAGAGG AGAGAGCCGCCCTCGAGCGGAGCAAGGCGATTGAGAAAAACCTCAAAGAAGATGGCATCAGCGCCGCCAA AGACGTGAAATTACTCCTGCTGGGGGCTGGAGAATCAGGAAAAAGCACCATTGTGAAGCAGATGAAGATC ATCCATGAAGATGGCTTCTCTGGGGAAGACGTGAAGCAGTACAAGCCTGTGGTCTACAGCAACACCATCC AGTCTCTGGCGGCCATTGTCCGGGCCATGGACACTTTGGGCGTGGAGTATGGTGACAAGGAGAGGAAGAC GGACTCCAAGATGGTGTGTGACGTGGTGAGTCGTATGGAAGACACTGAACCGTTCTCTGCAGAACTTCTT TCTGCCATGATGCGACTCTGGGGCGACTCGGGGATCCAGGAGTGCTTCAACCGATCTCGGGAGTATCAGC TCAATGACTCTGCCAAATACTACCTGGACAGCCTGGATCGGATTGGAGCCGGTGACTAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5686124), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59021. Forward Primer - name:059021_F_IRAV111_g10_Gnao1, sequence:TCGTGCCAGCCTAGTCGT; Reverse Primer - name:059021_R_SP6_IRAV111_g10_Gnao1, sequence:GGTAGTCACCGGCTCCAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15201 same embryo
	EMAGE:15199 same embryo
	EMAGE:15204 same embryo
	EMAGE:15203 same embryo
	EMAGE:15200 same embryo
	EurExpress:euxassay_010416 same experiment
	MGI:4825130 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source