EMAGE mouse gene expression spatial database

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EMAGE:15314

Dab1 disabled homolog 1 (Drosophila) ( MGI:108554)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010502. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010502_01	euxassay_010502_02	euxassay_010502_03	euxassay_010502_04

euxassay_010502_05	euxassay_010502_06	euxassay_010502_07	euxassay_010502_08	euxassay_010502_09

euxassay_010502_10	euxassay_010502_11	euxassay_010502_12	euxassay_010502_13	euxassay_010502_14

euxassay_010502_15	euxassay_010502_16	euxassay_010502_17	euxassay_010502_18	euxassay_010502_19

euxassay_010502_20	euxassay_010502_21	euxassay_010502_22	euxassay_010502_23	euxassay_010502_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15314_wholemount_strong.wlz

15314_wholemount_moderate.wlz

15314_wholemount_weak.wlz

15314_wholemount_possible.wlz

15314_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15314_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foot

moderate

regional

moderate expression: see section 09 10 11 22 23 24

mesenchyme

moderate

regional

moderate expression: see section 01 02 weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 13 14 15 16 weak expression: see section 11 12

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 13 14 15 16 weak expression: see section 11 12

rest of cerebellum mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 13 14 15 16 17 18 19 20 moderate expression: see section 10 11 12

pons mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 13 14 15 16 17 weak expression: see section 05 06 11 12

midbrain mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

basal columns

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15

naris

moderate

regional

moderate expression: see section 13 14 17 18

lower lip

moderate

regional

moderate expression: see section 07 08 18 19 20 21

upper lip

moderate

regional

moderate expression: see section 07 08 20 21

maxilla

moderate

regional

moderate expression: see section 13 14 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30551
Entity Detected:	Dab1, disabled homolog 1 (Drosophila) ( MGI:108554)
Sequence:	sense strand is shown >T30551 CACGGTGTTGGGTGTCCTTTCTGAAGGGAGGAGCCTTTCTCTTGGAGAGGATCCTCGATGAGCCTGGCCG AGGCCCGGGGTCTGTGTGAAGAGGACTAAGGATTAAGTAGGATGTCAACTGAGACAGAACTTCAAGTAGC TGTGAAAACCAGCGCCAAGAAAGACTCCAGGAAGAAAGGTCAGGATCGCAGCGAAGCCACTTTGATAAAG AGGTTTAAAGGCGAAGGGGTCCGGTACAAAGCCAAGCTGATTGGGATTGATGAAGTGTCCGCAGCTCGGG GAGACAAGTTATGTCAAGATTCCATGATGAAGCTCAAGGGTGTTGTTGCTGGCGCACGTTCCAAGGGAGA ACACAAACAGAAAATCTTTTTAACCATCTCCTTTGGAGGAATCAAAATCTTTGATGAGAAGACGGGGGCC CTTCAGCATCACCATGCTGTTCATGAAATTTCCTACATTGCGAAGGACATCACAGATCATCGGGCTTTCG GATACGTTTGCGGGAAGGAAGGGAATCACAGATTTGTGGCCATCAAAACAGCCCAGGCGGCTGAACCTGT TATCCTGGACTTGAGAGATCTCTTTCAACTCATCTATGAGCTGAAGCAAAGAGAAGAATTGGAAAAAAAG GCACAAAAGGATAAGCAGTGTGAACAAGCTGTGTACCAGACCATTTTGGAAGAGGATGTGGAAGATCCCG TGTACC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5692165), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59025. Forward Primer - name:059025_F_IRAV111_f01_Dab1, sequence:CACGGTGTTGGGTGTCCT; Reverse Primer - name:059025_R_SP6_IRAV111_f01_Dab1, sequence:TGGTACACGGGATCTTCCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15316 same embryo
	EMAGE:15312 same embryo
	EMAGE:15315 same embryo
	EMAGE:15311 same embryo
	EMAGE:15313 same embryo
	EurExpress:euxassay_010502 same experiment
	MGI:4824216 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source