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EMAGE:15315

Plscr4 phospholipid scramblase 4 ( MGI:2143267)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15315 EMAGE:15315 EMAGE:15315 EMAGE:15315 EMAGE:15315
"Pseudo-wholemount" of euxassay_010505. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010505_01 euxassay_010505_02 euxassay_010505_03 euxassay_010505_04
EMAGE:15315 EMAGE:15315 EMAGE:15315 EMAGE:15315 EMAGE:15315
euxassay_010505_05 euxassay_010505_06 euxassay_010505_07 euxassay_010505_08 euxassay_010505_09
EMAGE:15315 EMAGE:15315 EMAGE:15315 EMAGE:15315 EMAGE:15315
euxassay_010505_10 euxassay_010505_11 euxassay_010505_12 euxassay_010505_13 euxassay_010505_14
EMAGE:15315 EMAGE:15315 EMAGE:15315 EMAGE:15315 EMAGE:15315
euxassay_010505_15 euxassay_010505_16 euxassay_010505_17 euxassay_010505_18 euxassay_010505_19
EMAGE:15315 EMAGE:15315 EMAGE:15315 EMAGE:15315
euxassay_010505_20 euxassay_010505_21 euxassay_010505_22 euxassay_010505_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15315Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15315_wholemount_strong.wlz
15315_wholemount_moderate.wlz
15315_wholemount_weak.wlz
15315_wholemount_possible.wlz
15315_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15315_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
esophagus
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30549
Entity Detected:Plscr4, phospholipid scramblase 4 ( MGI:2143267)
Sequence:sense strand is shown

>T30549
CTGCTGGACCAGTTGCCTCTCCTTCTGCAGGCTTGCCTATGGGATACTACATTCCCCAGCAGCCCGGTGC
CATCCCCTTGTACCATCCCACTGGTGGCACCCATCCAATCCAGTACCAACCTGGCAAATATCCTGTGACG
AATCAGCCTGCTCCCATAATGTGGATGGCAGGGCCAGCTCCTGTGCCCAACTGCCCTCCTGGTTTGGAAT
ACTTAGCTCAGTTGGACAACATACACGTTCTTCAGCATGTTGAGCCTCTGGAACTGATGACACGTTTTGA
AACTAATAATAGATATGACATCAAGAACAACATAGACCAGATGGTCTATATCGTAACTGAAGACACAGAT
GACTTTACCAGGAATGCCTATCGGAACCTACGACCCTTTGTGCTCCGGGTCACTGACTGCCTGGGCCGAG
AGATCATGACCATGCAGAGGCCTTTCCGATGCACCTGCTGTTGCTTCTGCTGCCCCTGTGCAAGACAAGA
GCTGGAAGTGCAATGTCCTCCTGGGGTCACCATTGGCTTTGTTGCAGAACACTGGAACTTGTGCAGAGCC
TCTTATAGCATCCAGAATGAGAAGAAAGAGAGTATGATGAGAGTGAGGGGTCCGTGTGCAACCTACGGCT
GTGGCTCCGATTCTGTTTTTGAGATCAACTCTCTAGATGGCGTGTCTAACATCGGCAGTATTATAAGGAA
GTGGAATGGCTTTTTATCAACGATGGTAAATGCTGACCACTTTGAGATTCGCTTCCCTTTGGCCCTGGAT
GTGAAGATGAAAGCAATGATTTTCGGCTCTTGCTTCCTCATTGACTTCATGTACTTTGAACGACCTCCTC
CGCGACGTATG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5708712), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59118. Forward Primer - name:059118_F_IRAV111_e05_Plscr4, sequence:CTGCTGGACCAGTTGCCT; Reverse Primer - name:059118_R_SP6_IRAV111_e05_Plscr4, sequence:ACATACGTCGCGGAGGAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15316 same embryo
 EMAGE:15314 same embryo
 EMAGE:15312 same embryo
 EMAGE:15311 same embryo
 EMAGE:15313 same embryo
 EurExpress:euxassay_010505 same experiment
 MGI:4827287 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS