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EMAGE:15380

Samhd1 SAM domain and HD domain, 1 ( MGI:1927468)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15380 EMAGE:15380 EMAGE:15380 EMAGE:15380 EMAGE:15380
"Pseudo-wholemount" of euxassay_010565. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010565_01 euxassay_010565_02 euxassay_010565_03 euxassay_010565_04
EMAGE:15380 EMAGE:15380 EMAGE:15380 EMAGE:15380 EMAGE:15380
euxassay_010565_05 euxassay_010565_06 euxassay_010565_07 euxassay_010565_08 euxassay_010565_09
EMAGE:15380 EMAGE:15380 EMAGE:15380 EMAGE:15380 EMAGE:15380
euxassay_010565_10 euxassay_010565_11 euxassay_010565_12 euxassay_010565_13 euxassay_010565_14
EMAGE:15380 EMAGE:15380 EMAGE:15380 EMAGE:15380 EMAGE:15380
euxassay_010565_15 euxassay_010565_16 euxassay_010565_17 euxassay_010565_18 euxassay_010565_19
EMAGE:15380 EMAGE:15380
euxassay_010565_20 euxassay_010565_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15380Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15380_wholemount_strong.wlz
15380_wholemount_moderate.wlz
15380_wholemount_weak.wlz
15380_wholemount_possible.wlz
15380_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15380_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
weak weak
regionalweak expression: see section 08 09 10 11
left lung
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09
right lung
weak weak
regionalweak expression: see section 10 11 12 13 14 15 16 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30839
Entity Detected:Samhd1, SAM domain and HD domain, 1 ( MGI:1927468)
Sequence:sense strand is shown

>T30839
CTGGAGCGTCCCACAATCGCTTCGAACACAGTCTCGGAGTGGGGTACCTAGCAGGCTGCCTGGTGCGAGC
ACTTGCCGAAAAACAGCCAGAGCTACAGATCAGTGAGCGAGATATACTCTGTGTTCAGATTGCGGGGCTC
TGCCACGACCTAGGTCATGGGCCATTTTCTCATATGTTTGATGGAAGGTTTATCCCACGGGCTCGCCCAG
AGAAAAAGTGGAAGCACGAACAGGGCTCCATTGAGATGTTTGAGCATCTGGTCAATTCTAATGAACTCAA
ACTTGTCATGAAGAACTATGGTCTCGTCCCTGAAGAAGACATTACCTTTATCAAGGAACAAATTATGGGA
CCACCTATAACACCAGTCAAAGATTCCTTGTGGCCGTATAAAGGCCGCCCTGCCACGAAGAGCTTCCTTT
ACGAGATAGTGTCTAACAAGAGGAATGGCATCGACGTAGACAAATGGGATTATTTTGCCAGAGACTGTCA
CCATCTTGGAATCCAAAATAATTTTGATTACAAGCGCTTCATTAAGTTTGCCCGTATCTGTGAAGTGGAG
TACAAGGTCAAGGAGGACAAGACCTACATCCGTAAGGTGAAGCACATTTGTTCGAGAGAAAAGGAGGTTG
GAAATCTGTATGACATGTTCCACACTCGCAACTGCTTACACCGAAGAGCTTACCAACACAAGATCAGCAA
CCTCATCGACATAATGATTACAGATGCCTTCCTCAAAGCAGACCCCTACGTGGAGATTACAGGGACTGCC
GGGAAGAAGTTCCGCATTTCCACAGCCATTGATGACATGGAAGCCTTCACTAAGTTGACGGATAACATTT
TTCTGGAGGTTTTACACTCTACTGATCCACAGTTGTCTGAGGCCCAAAGTATTTTAAGGAACATTGAATG
CCGCAATCTATACAAGTATTTGGGTGAGACCCAGCCAAAGCGTGAGAAGATTAGGAAGGAAGAATATGAA
CGGCTTCCCCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4037046), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17345. Forward Primer - name:017345_F_IRAV20-23_L05_Samhd1, sequence:CTGGAGCGTCCCACAATC; Reverse Primer - name:017345_R_SP6_IRAV20-23_L05_Samhd1, sequence:TTGGGGAAGCCGTTCATA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15378 same embryo
 EMAGE:15377 same embryo
 EMAGE:15381 same embryo
 EMAGE:15379 same embryo
 EMAGE:15376 same embryo
 EurExpress:euxassay_010565 same experiment
 MGI:4827866 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS