EMAGE mouse gene expression spatial database

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EMAGE:15381

Cyp2f2 cytochrome P450, family 2, subfamily f, polypeptide 2 ( MGI:88608)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010560. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010560_01	euxassay_010560_02	euxassay_010560_03	euxassay_010560_04

euxassay_010560_05	euxassay_010560_06	euxassay_010560_07	euxassay_010560_08	euxassay_010560_09

euxassay_010560_10	euxassay_010560_11	euxassay_010560_12	euxassay_010560_13	euxassay_010560_14

euxassay_010560_15	euxassay_010560_16	euxassay_010560_17	euxassay_010560_18	euxassay_010560_19

euxassay_010560_20	euxassay_010560_21	euxassay_010560_22	euxassay_010560_23	euxassay_010560_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15381_wholemount_strong.wlz

15381_wholemount_moderate.wlz

15381_wholemount_weak.wlz

15381_wholemount_possible.wlz

15381_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15381_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

sublingual gland primordium

strong

regional

strong expression: see section 09 10 11 12

pharyngo-tympanic tube

strong

regional

strong expression: see section 06 07 08 09 15 16 17 18 19

anterior naris

strong

regional

strong expression: see section 14 16 17

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 12 13 14 16 17 18 19

pharynx epithelium

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

larynx

strong

regional

strong expression: see section 11 12

trachea

strong

regional

strong expression: see section 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30834
Entity Detected:	Cyp2f2, cytochrome P450, family 2, subfamily f, polypeptide 2 ( MGI:88608)
Sequence:	sense strand is shown >T30834 TGTGGACAAAGGGGAGGAGTTCAGTGGCCGAGGCGCATACCCCGTCTTTTTCAACTTCACCAGGGGCAAC GGCATCGCCTTCTCCGATGGAGAGCGCTGGAAGATCCTCAGAAGGTTCTCTGTCCAAATCCTGCGGAACT TTGGCATGGGAAAAAGAAGCATCGAGGAGCGGATCCTGGAAGAAGGCAGCTTCCTGCTGGAGGTGCTGAG GAAAATGGAAGGCAAGCCCTTTGACCCCGTGTTTATCCTGAGCCGCTCTGTGTCCAACATTATCTGCTCT GTCGTCTTCGGAAGTCGCTTCGACTATGACGATGAGCGTCTGCTCACCATCATCCACTTTATCAATGACA ACTTCAAGATTATGAGCAGCCCTTGGGGCGAGATGTACAACATCTTCCCAAGTGTCCTTGATTGGATACC TGGGCCACACAAACGCTTGTTCCGGAACTTTGGAGGCATGAAAGATCTCATTGCCCGCAGCGTCCGCGAA CACCAGGATTCCCTGGACCCCAACTCTCCCCGGGACTTCATCGACTGCTTCCTCACAAAGATGGCACAGG AGAAGCAAGACCCACTGAGCCACTTCAATATGGATACCCTGCTGATGACCACACACAACCTGCTCTTCGG TGGCACAGAAACCGTGGGCACCACACTGCGTCACGCCTTCCTTATTCTTATGAAATACCCCAAAGTGCAA GCCCGCGTGCAGGAAGAGATTGACCGTGTGGTGGGGCGCTCGCGGATGCCGACGCTGGAAGACCGTACAT CCATGCCTTACACAGATGCAGTGATCCACGAAGTGCAACGCTTTGCTGACGTCATCCCCATGAACCTGCC TCACCGTGTCACTCGGGACACACCTTTCCGGGGCTTCCTGATACCCAAGGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3968809), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 15390. Forward Primer - name:015390_F_IRAV20-23_I03_Cyp2f2, sequence:TGTGGACAAAGGGGAGGA; Reverse Primer - name:015390_R_SP6_IRAV20-23_I03_Cyp2f2, sequence:TGCCCTTGGGTATCAGGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15378 same embryo
	EMAGE:15377 same embryo
	EMAGE:15379 same embryo
	EMAGE:15380 same embryo
	EMAGE:15376 same embryo
	EurExpress:euxassay_010560 same experiment
	MGI:4824170 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source