EMAGE mouse gene expression spatial database

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EMAGE:15590

3110043O21Rik RIKEN cDNA 3110043O21 gene ( MGI:1920455)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010754. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010754_01	euxassay_010754_02	euxassay_010754_03	euxassay_010754_04

euxassay_010754_05	euxassay_010754_06	euxassay_010754_07	euxassay_010754_08	euxassay_010754_09

euxassay_010754_10	euxassay_010754_11	euxassay_010754_12	euxassay_010754_13	euxassay_010754_14

euxassay_010754_15	euxassay_010754_16	euxassay_010754_17	euxassay_010754_18	euxassay_010754_19

euxassay_010754_20	euxassay_010754_21	euxassay_010754_22	euxassay_010754_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15590_wholemount_strong.wlz

15590_wholemount_moderate.wlz

15590_wholemount_weak.wlz

15590_wholemount_possible.wlz

15590_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15590_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 03 weak expression: see section 04

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 04 05 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 17 18 21 22 weak expression: see section 04 05 06 07 08 16 19 20

vagus x ganglion

weak

regional

weak expression: see section 05

dorsal root ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14

anterior naris

weak

regional

weak expression: see section 12 13 14 15

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 17 weak expression: see section 09 10 11 12 13 15 16 18 19

lower jaw incisor

moderate

regional

moderate expression: see section 09 10 11 14 15

lower jaw molar

moderate

regional

moderate expression: see section 07 18

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

upper jaw molar

moderate

regional

moderate expression: see section 07

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31236
Entity Detected:	3110043O21Rik, RIKEN cDNA 3110043O21 gene ( MGI:1920455)
Sequence:	sense strand is shown >T31236 TCCCATGCTTACTGGGGAGGTCATTCCTGTAATGGAGCTGCTTGCATCTATGAAATCCCACAGTGTTCCT GAAGACATTGATATAGCTGATACAGTGCTCAATGATGATGACATTGGTGACAGCTGTCACGAAGGCTTTC TTCTCAATGCCATCAGCTCACACCTGCAGACCTGTGGCTGTTCTGTTGTAGTTGGCAGCAGTGCAGAGAA AGTAAATAAGATAGTAAGAACACTGTGCCTTTTTCTGACACCAGCAGAGAGGAAATGCTCCAGGCTGTGT GAAGCAGAATCGTCCTTTAAGTACGAATCGGGACTCTTTGTGCAAGGCTTGCTAAAGGATGCAACAGGCA GTTTTGTCCTACCCTTCCGGCAAGTTATGTATGCCCCGTACCCCGCCACGCACATTGATGTGGATGTCAA CACTGTCAAGCAGATGCCACCGTGTCATGAACATATTTATAATCAACGCAGATACATGAGGTCAGAGCTG ACAGCCTTCTGGAGGGCAACTTCAGAAGAGGACATGGCGCAGGACACCATCATCTACACAGATGAGAGCT TCACTCCTGATTTGAATATTTTCCAAGATGTCTTACACAGAGACACTCTAGTGAAAGCCTTCCTGGATCA GGTCTTCCATTTGAAGCCTGGCCTGTCTCTCAGGAGTACTTTCCTTGCACAGTTCCTCCTCATTCTTCAC AGAAAAGCCTTGACACTAATCAAGTACATCGAGGATGATACGCAGAAGGGGAAAAAGCCCTTTAAGTCTC TTCGGAACCTGAAGATAGATCTTGATTTAACAGCAGAGGGCGATCTTAACATAATAATGGCTCTAGCTGA GAAAATTAAGCCAGGCCTACACTCTTTCATCTTTGGGAGACCTTTCTACACTAGTGTACAAGAACGTGAT GTTCTAATGACCTTTTGACCGTGTGGTTTGCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4020832), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 54848. Forward Primer - name:054848_F_IRAV48_g11_3110043O21Rik, sequence:TCCCATGCTTACTGGGGA; Reverse Primer - name:054848_R_SP6_IRAV48_g11_3110043O21Rik, sequence:CAGCAAACCACACGGTCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15589 same embryo
	EMAGE:15587 same embryo
	EMAGE:15586 same embryo
	EMAGE:15585 same embryo
	EMAGE:15588 same embryo
	EurExpress:euxassay_010754 same experiment
	MGI:4822699 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source