EMAGE mouse gene expression spatial database

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EMAGE:16334

Zfp93 zinc finger protein 93 ( MGI:107611)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017179. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017179_01	euxassay_017179_02	euxassay_017179_03	euxassay_017179_04

euxassay_017179_05	euxassay_017179_06	euxassay_017179_07	euxassay_017179_08	euxassay_017179_09

euxassay_017179_10	euxassay_017179_11	euxassay_017179_12	euxassay_017179_13	euxassay_017179_14

euxassay_017179_15	euxassay_017179_16	euxassay_017179_17	euxassay_017179_18	euxassay_017179_19

euxassay_017179_20	euxassay_017179_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16334_wholemount_strong.wlz

16334_wholemount_moderate.wlz

16334_wholemount_weak.wlz

16334_wholemount_possible.wlz

16334_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16334_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6161
Entity Detected:	Zfp93, zinc finger protein 93 ( MGI:107611)
Sequence:	sense strand is shown >T6161 GCCCTGCCTTGTCTCAAAGAGAAGAGAACAATGACCAAGTTACAGGAGATGGTGACCTTCAGGGATGTGG CTGTTGTCTTCAGCGAGGAGGAGCTGGGGCTGCTGGACGCTGCTCAGAGGAAGCTGTACCACGATGTGAT GCTGGAGAACTTCAGGAACCTCCTGGCAGTGGGATGCCAGAGTCCAAATAAGATGGCGCCTCTTGACACA ACAGGAATAAGGTGCCTTCCATTGGGGCAGCTTCCATGTTGGCAAATGACCAGCCATGATGTCAACAAAC TAGCCAGAGCTCCAGAAGATGGAATAAACACTCCAGGAAAGGGTCCTCACCTCCTGGAGCAGTGCCACTC CTCCTGCCACTGGGGAGCAGAGCAGCCTTCTCAGGCCCCCGAGGATGACGGCTGTCTGGAGAATCTCCCA AGCAATCATTCCAGCAGTAGTGACAATCAGGAATTTCTCTCTGGGAGGGCTCAGAGTTCTTGGAGTAAGG CACACTTTAGTGAGAGATGGAACCATGAGAAACACTGTCCTCAGACTCTGGTGAAAACGAAGTCTCAGCT GTTGGCTCCAGGTGTTAACATTCTGGGTTGCATTTCCCACCACGATCACAATATACTGCATAAAAGAGAT AAAGTCCCCAGCAGCGGTGACTGTGATCAAGTCATTTTTCCCATGACGCTCCTCACCCAGCATTGTGTTT ATAGAGAACAGAAGGCCTACCAGTGCAGCAGGGGCCAAGAAGTCTTCAGTGACAGCCCTAGTCTGGAACT TCATCAACAGACCCTCTTAGGAAAGAAGTCCCCTGTGCATAGTACTCACAAGGACACCAGGCATAGCCCC AGTGTCCCCATTCAACCAAGTGTTCATCCAGGAAGAAAGCGCTACTGGTGTCATGAGTGTGGCAAAGGTT TCCGTCAGAGCTCAGCACTTCAGACTCACCAGAGAGTGCACACAGGGGAGAAGCCCTACAGGTGCGACAG CTGTGGGAAGGGCTTCAGCCGCAGCTCGGATCTCAACATCCACCGCCGAGTGCACACTGGAGAGAAACCT TACAAGTGTGAGGTGTGTGGGAAGGGCTTCACACAGTGGGCACACCTCCAGGCCCACGAGAGAATTCACA CAGGAGAGAAGCCATATAAGTGTGGAGACTGTGGCAAGCGCTTCAGCTGTAGCTCGAACCTCCACACCCA CCAGAGAGTCCACACTGAGGAGAAACCTTATGAGTGTAATGAGTGTGGGAAGCGCTTCAGCTTAAGTGGC AACCTAGACATCCATCAGCGGGTCCACACAGGAGAGAAGCCATATAAATGTGAAGAGTGTGGGAAGGGTT TCAGTTCAGCCTCAAGCTTCCAAAGCCACCAGAGGGTCCATACCGGAGAGAAGCCATTTCACTGCAGTGT CTGTGGGAAGAACTTCAGTCGGAGCTCACATTTTCTAGATCACCAAAGAATTCACACTGGGGAAAAACCG TATAGATGTGAAGTGTGTGGGAAGCGCTTCCCCTGGAGCTTGAGCCTTCATAGTCACCAGAGTGTGCACA CAGGAAAGAAACCGTATAAATGTGGGGAGTGTGGGAAAGGCTTCAGTCATGCCTCCAGTCTGCAGGCCCA TCACAGTGTCCACACCGGTGAAAAACCATTCAAATGCAATGTTTGCCAGAAGCAGTTCAGCAAGACCTCA AACCTCCAGGCCCACCAGAGGGTTCACACAGGGGAGAAGCCCTACAAATGCGACACGTGTGGGAAAGCCT TCAGCCAGAAGTCCAGTCTCCAAGTCCATCAGAGAATTCACACTGGGGAGAAGCCGTTCAAGTGTGAGGA GTGTGGGAAGGAATTCAGATGGAGCGTGGGGCTGAGTTCTCACCAGAGGGTCCACACAGGAGAGAAACCC TACACGTGTCAGCAGTGTGGGAAAGGTTTCAGTCAGGCCTCATATTTCCATATGCACCAGAGGGTCCACA CTATATAGAGACCTTAAATCGGCCATATTTGCTGTAGGGGCTATAGTCAGAGATCACATCTTGTCTTCCA CCAGAGAATCCACACTGCATGGAATCTTTAGTTTCTGCTGTCAGGTGGGCTCTTGGCTGATGATTGTTAA AAGTATCCCCAACACGCAGAGTCTTCAGGAGACTTCATAGGAGCTTTATTTTGGTATTTTTGCCCTCAAC TTATTCTCCTACTGTACTCATTATGCAAAGGGAAGAGAGTATTTTAGAATGGATCTTCTTAGCATATAAA TAAAAGAATTAAACGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3496816 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3496816 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16336 same embryo
	EMAGE:16335 same embryo
	EMAGE:16337 same embryo
	EurExpress:euxassay_017179 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source