EMAGE mouse gene expression spatial database

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EMAGE:16361

Ubap2 ubiquitin-associated protein 2 ( MGI:1916176)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017200. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017200_01	euxassay_017200_02	euxassay_017200_03	euxassay_017200_04

euxassay_017200_05	euxassay_017200_06	euxassay_017200_07	euxassay_017200_08	euxassay_017200_09

euxassay_017200_10	euxassay_017200_11	euxassay_017200_12	euxassay_017200_13	euxassay_017200_14

euxassay_017200_15	euxassay_017200_16	euxassay_017200_17	euxassay_017200_18

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16361_wholemount_strong.wlz

16361_wholemount_moderate.wlz

16361_wholemount_weak.wlz

16361_wholemount_possible.wlz

16361_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16361_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6157
Entity Detected:	Ubap2, ubiquitin-associated protein 2 ( MGI:1916176)
Sequence:	sense strand is shown >T6157 GGCCGCCTCTGCCGCCGCCGCCGCTTTGTAAGAGGCACATTGGCAGGTAACGAGCGGCGGCGGCGGCGGG TCCCGAGTCCAACGAGAGCAGCAGCGATTTTGTATTTTGTACATACATTTTGTATATTCTGTATATGATG ACTTCTGTGAGCAATGATCGTTGCCGAGGTGCTCGGGAAAAACCACAGATGCCAACAGCACACGCAGCCC AGTCACAGAAACAAGTGGTGCAGGCAACAGCTGAGCAGATGCGGCTTGCTCAAGTGATCTTTGATAAGAA TGATTCAGATTTTGAAGCTAAAGTTAAGCAGCTTATGGAAGTGACGGGAAAAAATCAGGATGAATGCATC GTGGCCCTACATGACTGTAATGGTGATGTGAACAAAGCTATCAATATACTGTTGGAAGGGAATTCTGACA CCACTTCATGGGAGACTGTAGGAGGGAAGAAGAAGAATTTTGGAAGAGAAAGTTCAGAAAACAAAGAAAA CAGAGAGAAGAGGACGGAGAGAGAAGCGAGTCGTGGACGGGGAACCAACAACCGCAAGGGAAGAGGCGGC AACCGAGTCCGAGAGTTCAAAGGTGAAGAGAATGGGATTGACTGCAGCCAAGGAGACAAGCCTGCGGAGC GGGGCAAGCGGGCCCGAGGCAGAGGATTTGGACGTGGCAGGGGCAGAGGGACAGGAAGGTTCTCCGCTCA AAGCATGGGGACATTCAATCCTGCAGACTATTCAGAGTCCATGTCTACAGATGGCTGTGGGACAAAGTTG GCAGTTTGGGAAGCTGCTCAGAATGGTACAGATGAGGGACCGGAAGGGCTGGCCAAGTCTCACAGTATGT CTCAGGAACCGCCAAGTAAAAGTTCTTATGGACTTAAAGGTGCCTGGAAGAATTCTGTGGAAGAGTGGAC AACAGAAGACTGGACTGAAGATCTCTCTGAAACAAAGGTATTCACTGCTTCATCTGCTCCAGCAGAGAAC CATGTCACACCTGGGCACAGCATTGATCTGGTGGCCTTGCTCCACAAGCCTGCTCCTCCCACCCAAGCCA CCGAAGTCAACTCCTTTGAAACATCCCAGCAGCAGGGCTTTGGCCAAGCCCTTGTCTTCACAAATTCTCA GCACAACAATCAGATGGCACCAGGGACTGCAAACTCCACTTCTGCCAGCTCATATTCTCCTCAGAGTTTG TCATCCGTCCTTGGCTCAGGATTTGGAGAGCTGCCTCAATCAAACATGGTGAACATCTCCAACTCCCAGA TTTTGGACAAGTTGAAACCGCCAGGTTTGAGCCCATTTCCCGCTGCTTCAAGCGCACAGCAGAATGACAC TGCGAGCCCCCCAGCCACCACTGCTGCTTGGGACCTCAAGCCCTCGGCGCCCCAACCCTCAGTCCTCAGT CGTTTGGACTTCAAATCTCAGCCTGAACCATCTCCAGTTCTCAGCCAGTTGAGCCAGCGACAGCAACACC AGACCCAGGCCGTCAGTGTCCCTCCTCCTGGTTTGGAGTCCTTTTCTTCTCTGGCAAAACCCCGAGAATC CACAGCTGGAGATGGTCCTTCTACTGTTAGCAGACTTTTGCAGCTTCCTAACATGACTGTTGAAAATATT GTATCTGCCCACCAACCACAGCCCAAACATATTAAACTTCCTAAGCGCCGGGTACCTCCAGCGTCTAAGG TTCCTGTGTCCGCAGTGGAAATGCCCGGGTCTTCAGATGTCACAGGGCTGAATGTTCAGTTTGGGGCCCT GGAATTTGGATCAGAGCCATCGCTCTCTGAGTTTGGATCAGCAGCTTCAGCCAGTGAAAATAGTAATCAG ATTCCTATCAGCTTGTACCCGAAGTCTTTAAGTGAGCCCTTGAATGCCTCTTTCCCAATGACCAGTGCTG TACAGAGCTCTACCTACACAACCTCAGTCGTTACCTCCTCCACTCTGACCAGCTCGGCCTTGAGCTCCAC TAGCCCAGTAACAACTTCTTCCTCTTACGACCAGAGTTCTGTGCATACCAGGATTGCATACCAAAGCTCT GCAAGCCCACCCGACTCAGCTCCAGGCTCTGTTGCTAACGGACATGGCGGTGGTCGAAGTCAGCACACAG TAGACACTACTTCATCCGTTCCAGCTCCAAAGAAGACAGACCCTTCCGCACTCCCATCTGTGAGCACCCT GCCCGGCCCCGCCTCCTGCACTGCACTTCTGCCCTCCTCAGCCCAGCACACTGCCACCTTGCCCTCCCTG ACCCCGGCTGCTGCTGAACTGTCCAGCAGCCCTCTCTCTCAGCTCAGCAGCTCCCTCTCTGGCCACCAGA ACAGCATGACCTCTGCACATGCGACACGTTCCACAAGCACGCCACACACTCATGCCAGCGTGGAGAGCAC CGCATCCTCCGCCGCCTTCTCAGCAGCGGCGACCTCTGCTCCAAGTGCCCCGTCCTCAGGGGTTGTCCTG CCTGGGAGCATGAGCACTGTGAGCAGCCTGTGCCTGGGGGGGACCACTGTGAGCGTCCCTAGCAGCAGCA CCAGGGCCACGGCCTTGGTGACCTCAGGCAAAGCACCCCCAAACCTGCCTCAAGGGGTGCCTCCCCTGCT ACACAATCAGTACCTCGTGGGCCCTGGAGGACTGCTTCCTGCTTACCCGATTTACGGTTATGATGAGCTC CAGATGCTGCAGTCACGGCTGCCAATGGATTACTATGGAATTCCCTTTGCAGCACCCACAGCCCTTGCCA GCCGAGATGGGAATCTAGCTAATAATCCATATTCAGGTGACGTCACAAAGTTTGGCCGAGGAGACTCTGC ATCCCCTGCACCTCCAACGACCCCAGCTCAGGCCCAGCAGAGCCAGTCTCAGACCCACCACACGGCCCAG CAGCCCTTTTTGAATCCTGGTCTGCCACCTGGCTACAGCTATACCGGCCTCCCCTACTACACAGGCGTGC CCAGTGCCTTCCAGTATGGGCCTACTATGTTTGTCCCTCCAACCTCAGCGAAGCAGCACGGTGTGGCCCT CAGCACTCCCCCGACCCCTTTCCAGCAGGCCAGTGGCTATGGGCAGCACGCTTACAGCACAGGTTATGAC GATTTGACCCAGGGGACGGCAGCAGGAGACTACACCAAGGGTGGCTATGGTGGATCATCTCAGGCACCAA ACAAGTCGACTGGTTCTGGGCCTGGCAAAGGAGTGTCAGTATCTTCAGGCACTGGCTTACCTGATATGAC TGGTTCCGTCTACAACAAGACACAGACTTTTGACAAGCAGGGATTTCATGCCGGGACTCCTCCACCATTC AGCCTGCCGTCTGCCTTGGGCTCCACCGGGCCCCTGGCCCCTGCAGCGGCCCCCGGCTACGCGCCTGCCC CGTTCCTGCACATCATGCCGGCCCACCAGCAGCCCCACTCCCAGCTGTTGCACCACCACCTACAGCAGGA TGCCCCGAGCGGCTCTGGTCAGCGCAGCCAGCCCAGCTCCCTTCAGCCCAAGTCTCAAGCCTCCAAACCC ACCTACGGCAGCGCTCCATACTGGACAAACTAGACCCTGAAAAGGAGGGGGTGGGCTCTGGGCAGGAGGG AACACATGGAGCACCTTCCTGGAGCCCCGTGCCCTTGCTGGGATCCTGAGACAACTGTCAGCTGAGCCCT GTGGGAAGCCTGCCTCCCAGACCCTCTGCTGTGTGTAATGTATTTATGTATGTACTTGTAAATGTGATAC AAGCCTGGAGGAGCTGGGCTGTGGTCACGGGTAGCACACAAGTGTGTGCTCCCAGTCCAGACACCCTTAC CATAGCCAAGTAGGCCCCACTCCGACCCCTCTGCCTTCAGATCTTCCATGTGGCCCCAGCTCAGTGGGCT GCTGGGTGTGCCACTGAAGGACTGGTGGTTTGGGGCCAGGTCAGTACCAATGAAGAATCACGACTTATCT CATTCCCTGTGAACCATTATGTTTTCTCCTCTGTAATTACTTCATCCTTGTGTTTGAAAAACTGATCCTT CTGTCATTTGTCATGGTCCCTCTTCTAACAAATCTTAATCTGGAAACAGAAGAAATAACCCCTGTCCAGC AGAAGCCTGTCCGTCTGCGCACAGCAGAAGCCTGTCCGTCTGCGCACAGCAGGGCTTCCGCTTTGGGTCT GGTCCTGCTCTGGGGACTTGTCTTTCTAACCAACATTTAAAAACAAAATCTGCACTAGGTCTCAGAGATG TGCAGCCAGTCAGCTCCCTTATAAAGAAATCAAAGATCTGCTTCCATCCATCTCAGTGAAAGCCTGTCTG CAATCAAAGGCTCCCCCATCCCAGTTGTCCTGAGCCAGGTGGACAGCTGTTTCAGGGTTTTCTCTCACCC AAACTCCACCTAATAAAATCCTGAGAGCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3496608 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3496608 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16362 same embryo
	EMAGE:16360 same embryo
	EMAGE:16363 same embryo
	EurExpress:euxassay_017200 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source