EMAGE mouse gene expression spatial database

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EMAGE:16362

Pds5b PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae) ( MGI:2140945)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017195. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017195_01	euxassay_017195_02	euxassay_017195_03	euxassay_017195_04

euxassay_017195_05	euxassay_017195_06	euxassay_017195_07	euxassay_017195_08	euxassay_017195_09

euxassay_017195_10	euxassay_017195_11	euxassay_017195_12	euxassay_017195_13	euxassay_017195_14

euxassay_017195_15	euxassay_017195_16	euxassay_017195_17	euxassay_017195_18	euxassay_017195_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16362_wholemount_strong.wlz

16362_wholemount_moderate.wlz

16362_wholemount_weak.wlz

16362_wholemount_possible.wlz

16362_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16362_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8738
Entity Detected:	Pds5b, PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae) ( MGI:2140945)
Sequence:	sense strand is shown >T8738 ATTTCCATCATGGCTCATTCAAAGACAAGGACCAACGATGGGAAAATTACTTACCCTCCTGGAGTCAAGG AAATCTCAGATAAAATCTCTAAAGAGGAGATGGTGAGGCGGTTAAAGATGGTTGTAAAAACTTTCATGGA CATGGACCAGGACTCTGAAGAGGAAAAGGAACTTTATCTAAACCTAGCTTTACATCTTGCTTCTGACTTC TTCCTCAAGCATCCTGATAAAGATGTTCGTTTACTGGTGGCTTGCTGCCTTGCTGATATTTTCAGGATTT ATGCTCCTGAGGCTCCTTACACGTCTCCCGATAAACTAAAGGATATATTTATGTTTATAACAAGGCAACT AAAGGGACTAGAAGATACAAAGAGCCCTCAATTTAATAGGTATTTTTATTTACTTGAGAACATTGCATGG GTTAAATCATATAACATATGCTTTGAGTTAGAAGATAGCAATGAAATCTTTACTCAACTTTACAGAACAT TATTCTCAGTTATAAACAATGGCCACAATCAGAAAGTTCATATGCACATGGTGGACCTCATGAGCTCTAT CATTTGTGAAGGTGATACCGTATCTCAGGAGCTCTTAGATACAGTTTTAGTAAATCTGGTACCTGCCCAT AAGAACTTAAACAAGCAAGCATATGATTTGGCAAAGGCTTTGCTGAAGAGGACTGCTCAAGCTATTGAAC CATATATTACCAATTTTTTTAACCAGGTTCTGATGCTTGGGAAAACATCTATCAGTGATTTGTCTGAGCA TGTCTTTGATTTGATCTTGGAACTCTACAACATTGACAGCCATTTGCTGCTTTCTGTTCTGCCCCAGCTT GAGTTTAAATTAAAGAGCAATGATAATGAAGAACGCCTCCAGGTTGTTAAACTACTGGCGAAAATGTTTG GGGCAAAGGATTCAGAATTGGCTTCTCAGAACAAACCCCTCTGGCAGTGCTACCTGGGCAGGTTTAATGA TATCCATGTACCAATACGCCTGGAATGTGTGAAATTTGCCAGCCACTGCCTCATGAACCATCCTGATTTA GCAAAGGATTTAACAGAGTATCTTAAAGTGAGGTCACATGATCCTGAGGAAGCTATTAGACATGATGTTA TTGTGTCTATAGTAACAGCTGCTAAAAAGGATATTCTTCTTGTCAATGATCACTTACTCAATTTTGTGAG AGAGCGAACATTGGACAAACGGTGGAGAGTACGTAAAGAAGCCATGATGGGACTGGCCCAGATTTATAAG AAATACTCTTTACAGTCAGCAGCTGGCAAAGATGCTGCAAAACAGATATCCTGGGTCAAGGACAAGCTGC TGCATATATACTATCAGAACAGCATCGACGACCGGCTACTTGTTGAGCGGATCTTTGCTCAGTACATGGT CCCTCACAATTTGGAAACGACGGAACGGATGAAGTGCTTGTATTACTTGTACGCTACACTGGACTTGAAT GCTGTGAAAGCATTGAATGAAATGTGGAAATGTCAAAATCTACTCCGACATCAAGTAAAGGATTTACTTG ATTTAATTAAACAACCAAAAACAGATGCCAGTGTCAAGGCCATATTTTCTAAAGTGATGGTTATCACAAG AAATTTGCCAGATCCTGGTAAGGCCCAAGACTTCATGAAGAAGTTCACACAAGTGTTGGAAGATGATGAG AAAATACGGAAACAGCTGGAAGCCCTTGTTAGTCCCACATGTTCCTGCAAGCAGGCTGAAGGCTGTGTGC GTGAAATAACTAAGAAGTTGGGCAACCCTAAGCAGCCTACAAACCCTTTCTTGGAAATGATCAAGTTTCT CTTGGAGAGGATAGCTCCTGTACACATAGACACTGAATCCATCAGTGCTCTTATCAAGCAAGTGAATAAA TCAATAGATGGAACAGCAGATGATGAGGATGAGGGTGTTCCAACTGATCAAGCTATCAGGGCAGGCCTTG AGCTGCTGAAGGTGCTGTCTTTTACGCACCCTATCTCCTTTCACTCTGCTGAGACGTTTGAGTCCCTTCT GGCTTGTCTGAAGATGGATGATGAGAAGGTGGCAGAAGCTGCACTACAAATTTTCAAAAACACAGGAAGC AAAATTGAAGAGGATTTCCCTCACATCAGATCAGCCTTGCTTCCTGTACTACATCACAAATCCAAAAAAG GACCACCTCGGCAGGCCAAATACGCCATCCATTGTATTCATGCCATATTTTCTAGTAAAGAAACCCAGTT TGCACAGATATTTGAGCCTCTGCATAAAAGCCTAGATCCAAGCAACCTGGAGCATCTTATAACCCCTCTG GTCACTATTGGTCATATTGCTCTTCTCGCACCTGATCAGTTCGCTGCTCCTCTGAAGTCTTTGGTGGCAA CTTTCATTGTGAAGGACCTCCTCATGAATGACCGGCTTCCAGGAAAAAAGACAACTAAGCTTTGGGTTCC AGATGAGGAAGTCTCACCTGAGACAATGGTCAAAATTCAGGCTATTAAAATGATGGTTCGATGGCTACTT GGAATGAAGAATAATCACAGTAAGTCAGGAACTTCCACCCTCAGATTACTAACAACGATACTGCATAGTG ATGGGGATTTGACAGAACAAGGGAAAATTAGTAAACCAGATATGTCACGCCTGAGACTTGCTGCTGGGAG TGCTATTGTGAAGCTGGCACAGGAGCCCTGTTACCACGAGATCATTACACTGGAGCAGTACCAGCTGTGT GCATTAGCCATCAATGATGAGTGTTATCAAGTCAGGCAGGTGTTCGCTCAGAAACTTCACAAAGGCCTTT CCCGCTTACGGCTTCCCCTTGAGTACATGGCCATCTGTGCTCTTTGTGCCAAAGACCCTGTGAAAGAGAG GCGAGCCCATGCTAGACAGTGCTTGGTGAAGAACATCACTGTGAGGAGGGAGTACCTGAAGCAGCATGCA GCTGTTAGTGAAAAATTATTGTCTCTTCTACCAGAGTATGTTGTTCCATATACAATTCACCTTTTGGCAC ATGACCCAGATTATGTCAAAGTACAGGATATTGAACAACTTAAAGATGTGAAAGAATGCCTTTGGTTTGT TCTGGAGATATTGATGGCTAAAAATGAAAACAACAGCCATGCATTTATCAGAAAAATGGTAGAAAATATT AAACAGACAAAAGATGCTCAAGGACCAGATGATACAAAAATGAATGAAAAATTGTACACCGTGTGTGATG TTGCCATGAACATCATCATGTCCAAGAGCACCACGTACAGCCTGGAGTCTCCTAAGGACCCCGTGCTGCC AGCTCGGTTTTTCACCCAGCCTGACAAGAATTTTAGTAACACCAAAAATTACCTGCCTCCAGAAATGAAA TCATTTTTCACTCCTGGAAAACCTAAAACAGCCAATGTTCTCGGAGCAGTTAATAAGCCACTTTCATCAG CAGGCAAACAGTCTCAGACCAAATCATCAAGAATGGAAACTGTGAGCAACGCAAGCAGCAGCTCCAACCC AAGCTCTCCTGGAAGGATCAAGGGGAGGCTTGATAGCTCTGAAATGGATCACAGTGAAAATGAAGATTAT ACAATGTCTTCACCTTTGCCAGGAAAAAAAAGTGACAAGAGAGAAGACCCTGATCTTTCTGAGTTGGAGA AGCCTAGAAGTCGGAAAAAAGCACCTGTCACAGACCCTGAAGAGAAATTAGGTATGGATGACCTAACTAA GTTGGTACAGGAACAGAAACCTAAAGGCAGTCAGCGAGGCCGGAAAAGAGGCCGTACAGCTTCAGACTCA GACGAGCAGCAGTGGCCTGAGGAGAAGAGGCACAAAGAGGAGCTCCTGGAAAATGAGGATGAGCAGAACA GCCCACCAAAAAAGGGCAAAAGAGGCAGGCCACCAAAACCTCTTGGTGGGGGGACATCGAAGGAAGAGCC CACAATGAAAACATCCAAGAAAGGAAACAAGAAAAAACTTGTACCTCCTGTAGTAGACGACGATGAAGAA GAAGAAAGACAAATTGGAAACACAGAACATAAGTCAAAAAGCAAACAGCACCGCACATCAAAGAGAGCAC AACAGAGAGCAGAATCTCCTGAAACAAGTGCAGTTGAATCCACACAGTCCACACCACAGAAAGGACGAGG AAGACCATCAAAAGCACCATCACCATCACAACCCCCCAAAAAAATCCGTGTAGGACGCTCCAAACAGGTA GCCACTAAAGAAAACGATTCAAGCGAAGAAATGGATGTGCTTCAGGCCAGCTCTCCTGTCAGTGACGACA CTACACAGGAAGGAGCAGAAGAGGAGGACATTTCTGTGGGAAATGTACGACGGCGAAGCTCCAAACGAGA GAGACGATGAACACGTCATTAACTTCTATTGTGAAAGCTTTGGGGGGAAAAAACATTTTTTGTCTAAATA TGAGGCTAAATAAAGCCTTGGATGCACAAACCAGAACTGCCAAAGCGTGGACAGTTGGACTTCGGATGAC CATGGGTTTGTGGTCACACGTATCAGCCATCCACACAGTGATAACACTGACTCCAGAGTCTTGTGAAAGT GTCCTGTGTGATGGCTGTGTAGACACAAACTAAAGAAGACACATGTAAATAATCCCACTCCCCCATTTCT GACTTCTAAAGTGCTTGTATAGCTTTTATCTGCGGCTTTAAACTGACAGGACCCAGCTGCTTACCGGATC TGTTCATTTGAAAAGCATTTGCTAGGGTGGATCTTAAGCAGTACGTAGTCTGTCAGTGTTTGTATTTGAA TTCTCTGCAATTTTACTGTGAAAATTTGTTTTCAACAATTGGTGTCATTTTCTCAATGTCACTGTTTGTT GGAGTTAAATGGTTTCTTCCTGCCCCTCTTTGTAGCTCATCTATGTTTACTCCAGGGCACTGTTGTCTTC TGAGGTGCTAAAGCATCTGCCAGCACACTAGAACATGCCTCTGTCTGGGGCACGACATGGATTGTGAGAT AGTCCTGAGCTTATCTGATGACTTCACACCTCAGTATTGGTCCCAGAATTCTCTGGCCTTTCATGGCAAC GAAAATTTTAAGAAAGAGATTTAAAATATTTTAATTTTAAAGAGTGTGTTATAAAATAATGTACTAAATT CTTTATTCCATTCTATCATCTTTTTTCAGTTTATATTAATCTACTGTATCAATAAAATTCTGTAATTTGA ATGGGCTTTTAATAGTCTAGAATGTTATTGTGTATAGATATTACTTCTTGAACATTATGTTCAGAAATGC AAATTATTACACTATAATATAAAATCTGATATATACACGTTAGGAAAGTTCTCATTCTCCATAACCATGT AAAGTTAATCAGAAACAAAAAAATTTTATTGATGCAGTGTATCTTTATAAATCTGTTCTTGAAAGCCAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6405163 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6405163 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16360 same embryo
	EMAGE:16361 same embryo
	EMAGE:16363 same embryo
	EurExpress:euxassay_017195 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source