EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:16365

Hlx H2.0-like homeobox ( MGI:96109)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017173. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017173_01	euxassay_017173_02	euxassay_017173_03	euxassay_017173_04

euxassay_017173_05	euxassay_017173_06	euxassay_017173_07	euxassay_017173_08	euxassay_017173_09

euxassay_017173_10	euxassay_017173_11	euxassay_017173_12	euxassay_017173_13	euxassay_017173_14

euxassay_017173_15	euxassay_017173_16	euxassay_017173_17	euxassay_017173_18	euxassay_017173_19

euxassay_017173_20	euxassay_017173_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16365_wholemount_strong.wlz

16365_wholemount_moderate.wlz

16365_wholemount_weak.wlz

16365_wholemount_possible.wlz

16365_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16365_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8274
Entity Detected:	Hlx, H2.0-like homeobox ( MGI:96109)
Sequence:	sense strand is shown >T8274 GCTGTTTGGGTTTTTCTTTCGGTGTCGGGTCCCTGTCTCCCCGGCTTCTGCGGAGTAGCAGAGCGAGGCC GCGCTCGCCTTGTCGCCCCAGCGCCTGGCGATCGCCTCCATCCACCCGGCGGGGAGCGCGCCCGCCGCGC CGCCTTTCAAGCCAAGCCAGGGACCCAGGCCGTGGCGGGCGGCGATCGGGCCCTCGCCTCGTCCCTCGGT GGCGCTAGGGCTCCCCGGTTGGCCCTCCGCTTCAGGGCGGGCGAAGAACCGAAAGCAGCTCGGGCTCGGC TGGGACACCGCCTCTGCCCGAGCGCCCACCCACTTCCCCACTCACCCAGGACCCCGCCGGGATCCTGCCA GGATGTTCGCAGCCGGCCTGGCTCCCTTCTATGCTTCCAACTTTAGTCTCTGGTCAGCCGCCTACTGCTC CTCGGCGGGCCCGGGCGGCTGCTCCTTCGCCCTGGACCCAGCCGCTGTCAAGAAACCCTCTTTCTGCATC GCGGACATCCTGCACGCCGGCGTCGGGGAGCCGGGGCCCGCTGCAGAGGGACTGGTGGGGGCCTCGGCAG CCCTCACCGCACACTTGGGCTCCGTGCACCCGCACGCCTCCTTCCAAGCTGCCGCCAGATCCCCGCTCCG TCCCACCCCGGTGGTGGCGCCCTCAGAAGTCCCGGCTGGCTTCCCTCAGCGGCTGTCTCCGCTCTCTGCC GCCTTCCACCAGCATCTCCCGCAGCAATCACCAACGCAGCAACAGCAGCCGCAGCAGCAGCCCCCGCCTC CACCCCGGGCGGTCTCCCTGCAGCCACCGACTTCGGGGACGCGGGTGGTCCCCCACCATAGCGGCTCCGC CCCTGCCCCCTCCAGCAAAGACCTCAAATTTGGTATCGATCGAATTTTGTCTGCGGAATTTGACCCAAAA GTTAAGGAAGGCAACACTCTGAGAGATCTCACATCGCTGCTAACCGGTGGGCGGCCTGCAGGGGTGCATC TAGCTGGCCTGCAGCCTTCAGCGGGACAGTTCTTCGCATCTCTAGATCCCATTAGCGAGGCGTCTGCCAT CCTCAGCCCCTTAAGCTCCAACCCAAGAAATTCTGTTCAGCATCAATTCCAAGACACATTTCCAGGTCCC TATGCTGTGCTCACTAAGGACACCATGCCACAGACATACAAGCGGAAGCGCTCGTGGTCCCGTGCTGTCT TTTCCAACCTGCAAAGAAAAGGCCTGGAGAAGAGGTTTGAGATCCAGAAGTACGTGACCAAGCCAGACCG AAAGCAGCTGGCGGCCATGCTGGGCCTCACGGATGCACAGGTGAAGGTGTGGTTCCAGAACCGGAGGATG AAGTGGCGGCACTCTAAGGAGGCGCAGGCGCAGAAGGACAAGGACAAGGAAGCGGGCGAGAAGCCTTCCG GCGGAGTCCCCGCCGAGGGTGAACGGGAGGAGAGGAGCCCCAGCCGTTCTGAGGGCGAAGCCGAGAGCGA GAGCAGTGACTCGGAGTCCCTGGACATGGCCCCCAGCGACACCGAACGGACTGAGGGAACGGAGAGGTCC CTGCATCAAACCACGGTCATCAAGGCCTCAGCCGCGGGTGCCCTCATCACTGCCAGCAGCAGCACGAGTG GTAGCAGCTTCAGTTTCAGCAGCACCAGCAGCCTGGGTAGCGGCAACACCCACGTGGGGAGCGCCAGCAG CCTTGGCGGCAACTGCTCGGAGCTGCCTTCGGCTCACCAGCCCTCAGTCACCAGCAGTCCTCAAAGCCGG GAGATCGCCCAGGCCCCGCTTGCAGGTTTATAGACTGGACCGAGGGCATAGCGTGCAGCTTCCTCTACAC TGGCTGGACTTTGTGCCTACTTTAAGTTGGTGGCGAGGGTTGCCTAGATTGGAGCCGGACGCCACGGGCA GCCTCTTCGGGGTTCATACTTTTTCTCTTGCTGCCCCGGCTGCCAGAGCCCCCACCCCCGTCCGCCCAGT GGAGAGTTTTAAGGTTCCCCCTCCCCCCGCCACAAAGAAAGAATTCAACTGTTTTCCCTACCTAAGCAGC TCACACTGTGAAATGTTCGGTGGAACTGTTCCTCTGAGCACCAGGGGTCTTGGCATAAGGACTTGCTGCA CTTAGGAGGCCTTTAACTTGTAAATAAAATGTTTACTACGGTTTGTAAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:1380899 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1380899 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16369 same embryo
	EMAGE:16366 same embryo
	EMAGE:16368 same embryo
	EMAGE:16367 same embryo
	EMAGE:16364 same embryo
	EurExpress:euxassay_017173 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source