EMAGE mouse gene expression spatial database

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EMAGE:16367

Mme membrane metallo endopeptidase ( MGI:97004)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017187. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017187_01	euxassay_017187_02	euxassay_017187_03	euxassay_017187_04

euxassay_017187_05	euxassay_017187_06	euxassay_017187_07	euxassay_017187_08	euxassay_017187_09

euxassay_017187_10	euxassay_017187_11	euxassay_017187_12	euxassay_017187_13	euxassay_017187_14

euxassay_017187_15	euxassay_017187_16	euxassay_017187_17	euxassay_017187_18	euxassay_017187_19

euxassay_017187_20	euxassay_017187_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16367_wholemount_strong.wlz

16367_wholemount_moderate.wlz

16367_wholemount_weak.wlz

16367_wholemount_possible.wlz

16367_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16367_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4158
Entity Detected:	Mme, membrane metallo endopeptidase ( MGI:97004)
Sequence:	sense strand is shown >T4158 GTGACCGAGAGCAGCGCTGCCAAATTGCACCGGGGTTCATTTGGGGGCTGGGAATTGGCCATTCCGCTGT ACAGACACTGTTTTTATTTTAAAAAACAAGATTTTAGGTGATGGGAAGATCAGAAAGTCAGATGGATATA ACTGATATCAATGCTCCAAAGCCAAAGAAGAAACAGCGATGGACTCCCCTGGAGATCAGCCTCTCTGTGC TTGTCTTGCTCCTGACTATCATAGCGGTGACAATGATCGCTCTCTATGCAACCTATGATGATGGCATTTG CAAATCATCAGACTGCATAAAATCAGCTGCTCGACTGATTCAGAATATGGATGCCTCTGTTGAGCCATGT ACAGACTTCTTCAAGTATGCTTGTGGAGGCTGGTTGAAACGCAACGTCATTCCCGAGACCAGTTCCCGAT ACAGTAATTTTGACATTTTAAGAGATGAGCTAGAAGTCATTTTGAAAGATGTCCTTCAAGAACCCAAAAC TGAAGACATAGTAGCAGTGCAGAAAGCAAAAACCTTGTACAGATCGTGTATAAATGAGTCTGCTATTGAT AGCAGAGGCGGACAACCTCTACTCAAACTGTTACCAGATATATATGGGTGGCCAGTAGCATCAGATAACT GGGATCAAACATATGGCACTTCTTGGACAGCTGAGAAATCCATTGCACAACTGAATTCTAAATATGGGAA AAAGGTCCTCATTAATTTTTTTGTTGGCACTGATGATAAGAATTCTACCCAGCATATAATTCATTTTGAC CAGCCTCGACTTGGCCTCCCTTCCAGAGACTACTATGAGTGCACTGGAATATATAAAGAGGCTTGCACAG CATATGTGGATTTTATGATTTCTGTGGCCAGACTGATTCGTCAGGAACAAAGTTTGCCTATTGATGAAAA CCAGCTCTCTTTGGAAATGAATAAAGTTATGGAATTGGAAAAAGAAATTGCCAATGCTACAACTAAACCA GAAGACCGAAATGACCCAATGCTGCTTTATAACAAAATGACATTGGCCAAGCTGCAGAACAACTTTTCCC TGGAGGTCAATGGGAAGTCATTCAGCTGGTCAAATTTCACAAATGAAATCATGTCAACTGTGAATATTAA TATTCAAAATGAGGAAGAAGTTGTTGTCTATGCTCCAGAATATTTAACCAAACTTAAGCCTATTCTTACC AAATATTCTCCCAGAGATCTTCAAAATTTAATGTCCTGGAGGTTCATAATGGATCTTGTAAGCAGCCTCA GCCGAAACTACAAGGAGTCCAGAAATGCTTTCCGCAAGGCCCTTTATGGGACTACATCAGAAACTGCAAC ATGGAGACGGTGTGCTAACTACGTCAATGGGAATATGGAGAATGCTGTGGGGAGGCTTTATGTGGAAGCG GCTTTTGCTGGAGAGAGCAAGCACGTGGTTGAAGACTTGATTGCACAAATCCGGGAAGTTTTTATTCAGA CTTTGGATGACCTTACTTGGATGGATGCTGAGACAAAAAAGAAAGCTGAAGAAAAGGCCTTGGCAATTAA AGAAAGGATTGGCTATCCTGATGACATCATTTCCAATGAGAACAAACTGAATAATGAGTATCTTGAGTTG AACTACAGGGAAGATGAATACTTTGAGAACATAATTCAAAATTTGAAATTCAGCCAAAGCAAGCAGCTAA AGAAGCTCCGAGAAAAAGTGGACAAAGATGAGTGGATAAGTGGAGCAGCTGTAGTCAATGCATTTTATTC CTCAGGCCGAAATCAGATAGTCTTCCCCGCTGGCATTTTGCAGCCCCCATTCTTCAGTGCTCAGCAATCC AACTCATTGAACTATGGGGGCATCGGCATGGTCATTGGACATGAAATCACACATGGATTTGATGACAATG GAAGAAATTTCAATAAAGATGGAGACCTCGTTGACTGGTGGACGCAGCAGTCGGCAAATAATTTTAAAGA CCAATCCCAGTGTATGGTATACCAGTATGGAAACTTTTCCTGGGACCTAGCAGGTGGACAGCATCTCAAT GGAATTAATACACTAGGAGAAAACATTGCTGATAATGGAGGTATTGGCCAAGCATACAGAGCCTATCAGA ATTATGTTAAAAAGAATGGTGAAGAAAAATTACTCCCTGGACTTGACCTCAATCACAAACAACTATTTTT CCTGAACTTTGCCCAGGTGTGGTGTGGGACCTACCGGCCAGAGTATGCAGTCAATTCTATTAAAACAGAT GTACACAGTCCTGGAAATTTCAGGATCATTGGGACTTTGCAGAACTCTGCCGAGTTTGCAGATGCCTTTC ATTGCCGCAAGAATTCATACATGAATCCAGAAAGGAAATGTCGGGTTTGGTGATCTTCACAAGATACTGA ACATCCACGGCAAGATTTGCCAAAGCCACGGAAACAGGAACTCTTCCCTCAGAGAAACTGGGCCCCGGAA GTTTCTTCAGCTTCCTGGGGGGCAATTCACAGAGACGAGCATGAGCTAACATAAATGAAATTAGATTATT AAATCTGCTGTGAATGAAAGGGGAGGGAGCCTACGATCTAGCAAATCAATCACTTCACTGTGTAAATAAT GATTACTTTCCAACGGTAGTACTACCGTTCACTTCTGTTTCTCACACAGACTGCAGCTTTCATGTTGTCT GTAGAGAGGAGTGTTAACACTTAAAGCAGACTATGACTTCTGATCAAAAAGAGGACGATGCTCAGTACAG CAGGGCACCAAAGCACAGATTTGCCCCTCAGCACTCACTTTTGTTTGCAACATTCATGCTCCTTCCAAAC TCTCCCAAAGAACTCCCATACAGACTGTGGCCTTCAGGCTCGTGCAGTTTTGAACTCATTTTACCATACA TAAGTTATTCATTCATTCCACATCAATTTAGTTTGAACACTCTTAGAGCTTAAACTAGAGTATCTGAAAC GGTTCCCCTATTTCACCCACTTTAGTGATGTTGAGATTCTTGAACCCCCCTGCAGATCTTTGAGCAATTT CTTGCTCTTGCTGCCCCTCAGACTCAGTCTTTTAAAGGATTTGTAGTAATGTATAAAGAATAATTCTATA TTTAATTATTAACTACATTTATGACTGAATAAACCATTGCTATAGGTAATCATTGAATATTGAAGTTATA TGGCCAAGATAGTTTTGAAGATCTGTAACATGATATACAGACAAAAATTTGAGACTCTTTAAATCTGTAA ATCATATTGATGAAAAGCTTTAAGTACAAACTCTGGGGGTGAGCATTACCATTGGACGGGTGTCTTGAGA TACATAATAGTTGTGGTTTACAAACAGCATTTTCAAGATTAAGTCTGTCCCTAGTGGCTAAGAAAGGGTC TAACTGTATCTGTGTTGCTCCATGAATGCTTAGCCTTCAGGTTTGTCATCAAATGAAAATATTTTGATAA TAAATTGACGTTGTGAGCCCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4483558 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4483558 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16365 same embryo
	EMAGE:16369 same embryo
	EMAGE:16366 same embryo
	EMAGE:16368 same embryo
	EMAGE:16364 same embryo
	EurExpress:euxassay_017187 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source