EMAGE mouse gene expression spatial database

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EMAGE:17064

Col14a1 collagen, type XIV, alpha 1 ( MGI:1341272)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001452. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001452_01	euxassay_001452_02	euxassay_001452_03	euxassay_001452_04

euxassay_001452_05	euxassay_001452_06	euxassay_001452_07	euxassay_001452_08	euxassay_001452_09

euxassay_001452_10	euxassay_001452_11	euxassay_001452_12	euxassay_001452_13	euxassay_001452_14

euxassay_001452_15	euxassay_001452_16	euxassay_001452_17	euxassay_001452_18	euxassay_001452_19

euxassay_001452_20	euxassay_001452_21	euxassay_001452_22	euxassay_001452_23	euxassay_001452_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17064_wholemount_strong.wlz

17064_wholemount_moderate.wlz

17064_wholemount_weak.wlz

17064_wholemount_possible.wlz

17064_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17064_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 20 21 22 23 24

head mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 19 20 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22 23 24

pectoral girdle and thoracic body wall musculature

strong

regional

strong expression: see section 13 14 15 16 17 18 19

foregut-midgut junction

strong

regional

strong expression: see section 12 13 14 15 16 17 18

vibrissa

strong

regional

strong expression: see section 05 06 07 08 09 10 21 22 23 24

stomach

strong

regional

strong expression: see section 04 05 06 07 09 10 11 moderate expression: see section 03

hindgut

strong

regional

strong expression: see section 15

midgut

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19 20 21 22

chondrocranium

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

orbito-sphenoid

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19

viscerocranium

strong

regional

Expression in the turbinate bone.

tail paraxial mesenchyme

strong

regional

strong expression: see section 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1233
Entity Detected:	Col14a1, collagen, type XIV, alpha 1 ( MGI:1341272)
Sequence:	sense strand is shown >T1233 TCCTCGAGCCTGTTGGCCTACTGGCCGCTAGTTGGGATCATATTGGACAATGGTGGGAAAACTCTAACTT ATTTCAACTATGACTATACTGGAGATTTTCAAACTGTCACTTTTGAAGGACCCGACATCAGGAAAATGTT CTATGGAAGCTTTCACAAGCTCCATGTTGTTGTCAGCAAGACTCTGGCCAAAGTGGTTGTTGACTGCAAG GAAGTTGGTCAGAAGGCAATAAATGCATCAGCGAATATCACATCGGATGGTGTGGAAGTACTAGGAAGAA TGGTCAGATCAAGAGGACCAAATGGAAACTCTGCACCATTCCAGTTACAAATGTTTGATATCGTTTGCTC TACATCATGGGCCAGTAAAGACAGATGCTGCGAACTTCCTGGTCTGCGGGATGAAGAGTCCTGCCCAGAC CTTCCACGGTCCTGCTCTTGCTCGGAAACCAATGAAGTGGCTCTAGGACCAGCTGGCCCCCCAGGTGGTC CAGGACTCCGAGGACCAAAAGGCCAACAAGGTGAACAGGGTCCGAAGGGACCAGAGGGACCTCGGGGAGA AACAGGTCCTGC
Notes:	The probe template was PCR amplified from IMAGE:2192739 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2192739 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17065 same embryo
	EMAGE:17066 same embryo
	EMAGE:17063 same embryo
	EMAGE:17062 same embryo
	EurExpress:euxassay_001452 same experiment
	MGI:4823977 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source