EMAGE mouse gene expression spatial database

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EMAGE:17096

Prpsap2 phosphoribosyl pyrophosphate synthetase-associated protein 2 ( MGI:2384838)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001483. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001483_01	euxassay_001483_02	euxassay_001483_03	euxassay_001483_04

euxassay_001483_05	euxassay_001483_06	euxassay_001483_07	euxassay_001483_08	euxassay_001483_09

euxassay_001483_10	euxassay_001483_11	euxassay_001483_12	euxassay_001483_13	euxassay_001483_14

euxassay_001483_15	euxassay_001483_16	euxassay_001483_17	euxassay_001483_18	euxassay_001483_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17096_wholemount_strong.wlz

17096_wholemount_moderate.wlz

17096_wholemount_weak.wlz

17096_wholemount_possible.wlz

17096_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17096_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 15 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 15 16 17 18 19

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 15 16

spinal cord

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 11 12 13

cervical ganglion

moderate

regional

moderate expression: see section 07

thoracic ganglion

moderate

regional

moderate expression: see section 09 10

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 14 15

neural retina

moderate

homogeneous

moderate expression: see section 01 02 03 19

renal cortex

moderate

regional

moderate expression: see section 15 16 weak expression: see section 06 07 08 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4463
Entity Detected:	Prpsap2, phosphoribosyl pyrophosphate synthetase-associated protein 2 ( MGI:2384838)
Sequence:	sense strand is shown >T4463 CGACGCGTGGGCTTTCTCGCGCTTAGAGTCGCCATTTTGCTGTAAGACGCCGTGCGGAGCTCTGTGCTCT CTTCCTTCGGCAGCCGAGGCTGCGGCGGGCCGGGCTGGGGTCGGGACCAGGAGGAATTTTGTTGTCAGAG AATAACAGGAGGTTGTCCATAATTGACTTTAAGCAGCAAACAGTAAAACACTGAGCTCTTCAGCTCCGCC TTTCTTGCTCTGAGAATTGGAAAGCCAAGAAGGTTTTGATGTTTTGTGTGGCGCCTCCTGAATTAGAAAC CAAGATGAACATAACCAAAGGAGGCCTCGTGTTGTTTTCAGCAAATTCCAATTCATCATGTATGGAGCTT TCAAAGAAAATTGCTGAGCGGCTGGGGGTGGAGATGGGCAAAGTGCAGGTTTACCAGGAACCTAACAGAG AAACAAGAGTTCAAATCCAGGAGTCTGTGAGAGGAAAAGATGTATTTATCATCCAAACCATCTCAAAGGA CGTGAACACCACCATCATGGAGCTGCTGATCATGGTATATGCATGTAAGACCTCATGTGCCAAGAGCATC ATCGGAGTGATCCCCTACTTTCCTTATAGCAAGCAATGCAAAATGAGAAAAAGGGGCTCCATTGTCTCAA AATTGCTGGCTTCTATGATGTGCAAAGCTGGTCTGACTCATCTCATCACTATGGATTTACACCAGAAGGA AATCCAGGGCTTCTTCAATATTCCAGTGGATAATTTAAGAGCCTCCCCATTCCTGTTACAATATATTCAA GAAGAGATCCCAGATTATAGGAATGCAGTCATCGTGGCCAAGTCTCCAGCCTCAGCCAAGAGGGCACAAT CTTTTGCAGAGCGTCTACGTCTGGGAATCGCAGTGATCCATGGAGAAGCACAGGATGCTGAGTCTGACTT GGTGGATGGCCGGCACTCCCCACCAATGGTCAGGAGTGTAGCTGCCATCCATCCCAGCCTGGAGATCCCG ATGCTGATTCCTAAAGAGAAGCCCCCAATCACAGTTGTTGGTGATGTTGGAGGAAGAATCGCCATCATTG TGGATGACATTATTGATGATGTGGATAGCTTTCTTGCTGCAGCAGAGACCCTGAAGGAAAGAGGTGCATA CAAGATCTTTGTGATGGCCACTCACGGCTTGTTATCCTCTGATGCTCCCCGGCTGATTGAAGAGTCTGCC ATTGATGAGGTGGTGGTCACTAACACGATCCCACACGAGATCCAGAAGCTGCAGTGCCCCAAGATCAAGA CTGTGGACATCAGCATGATCCTCTCAGAAGCCATCCGCCGAATTCACAACGGGGAGTCCATGTCCTACCT TTTCAGAAACATAGGCTTAGATGACTGAACAGTTCTCCTATGGTAGAACTCCCACAGGTCAAGTGGGAGC CCAGCGTGTACGCTGGGAGGGCCCATGCCCGGCCAGACACACGTGACCCTCCCTTTCCCACATCTTCCTC ATTCATTCCTGTGAAGGTACAAGGACCAGCTTTTTAATGTTGGTTTAGGTTTTTGTGAGTTTTAGGGGTA ATTTTTATAACAGAAAGACCTCCTCTGAAATGAAAGAAGTTAAAACCTGATTGTTTTTAGTGTAACCATT TTAAATAATACACTTGTGAGATTTTTAAGCTCAGCCTTTTTTTAAAGTTGCTTGATCCAACTGTTCAAAA GAATTAAATAAAATGATCTGCTGTCTACTACCTAGAATCAAAAAGCTACTGTTGGTTCCAACACATGACC TTTTTAGGAACGCTTTTATAAATGGGCATATAGAGTATGTGGCTGTATTTATTTTCTGCAACTAAAAAAA GAATAAAACCTTGTGTTTGTTTTTTAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4947226 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4947226 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17094 same embryo
	EMAGE:17092 same embryo
	EMAGE:17097 same embryo
	EMAGE:17093 same embryo
	EMAGE:17095 same embryo
	EurExpress:euxassay_001483 same experiment
	MGI:4827446 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source