EMAGE mouse gene expression spatial database

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EMAGE:17227

Wipf2 WAS/WASL interacting protein family, member 2 ( MGI:1924462)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008874. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008874_01	euxassay_008874_02	euxassay_008874_03	euxassay_008874_04

euxassay_008874_05	euxassay_008874_06	euxassay_008874_07	euxassay_008874_08	euxassay_008874_09

euxassay_008874_10	euxassay_008874_11	euxassay_008874_12	euxassay_008874_13	euxassay_008874_14

euxassay_008874_15	euxassay_008874_16	euxassay_008874_17	euxassay_008874_18	euxassay_008874_19

euxassay_008874_20	euxassay_008874_21	euxassay_008874_22	euxassay_008874_23	euxassay_008874_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17227_wholemount_strong.wlz

17227_wholemount_moderate.wlz

17227_wholemount_weak.wlz

17227_wholemount_possible.wlz

17227_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17227_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5641
Entity Detected:	Wipf2, WAS/WASL interacting protein family, member 2 ( MGI:1924462)
Sequence:	sense strand is shown >T5641 GACTAAACCAGGACTGAGGTGACGGAGGATTCGCTCCCGGAGCAGCCCCCCGCCGAGTGGGAAAGACGCC GGGGCGGCTGAGCCCGCCGCTGAGGTATATGAATGACCTAAAGCGGCAAATAAAGACGGAGAGACAACGG TGCCAACTGGAGCAGGGCAAGAATGCCAATTCCTCCTCCCCCTCCGCCCCCGCCTGGTCCTCCCCCACCT CCCACATTTAATCAGGCAAACACAGAACAGCCCAAACTCAGTAGAGATGAGCAGCGGAATCGAGGCGCCC TCTTACAAGACATTTGCAAAGGGACCAAACTAAAGAAGGTGACCAACGTTAATGATCGGAGTGCTCCTGT CATCGAGAAGCCCAGAGGGAGCAGTGGTGGCTACGGCCCCGGAGCTGCTGCTCTGCAGCCCAAAGGAGGT CTCTTTCAAGGAGGAGTGCCGAAGCTCCGACCTGTGGGAGCCAAGGATGCTTCAGAGGCTCCAGCTGGTA AGCCGGCCCTGCAAGTCCCCAGCTCTCGAGCTGCTGCTCCAAGGCCTCCGGGGTCTGCAGCCAGTGGGCG TCCTCACGATGATACAGACAGCAACCGAGCCTCCCTCCCAGAACTGCCCCGGATGCAGAGACCCTCCTTA CCGGACCTCTCTAGGCCCAACACCGCCAGTGGCACAGGCATGAAGCACAGCTCCTCTGCACCTCCCCCAC CACCTCCAGGGCGGCGTGCCAACGCGCCCCCCACCCCTCTGCCTCTGCACAGCAACAAAGCCCAAGCCTA CAACAGGGAGAAACCATTGCCTCCAACACCTGGACAGAGGCTGCACCCTGGACGAGAAGGACATCCTGCT CCACCCCCTGTAAAGCCACCTCCTTCCCCTGTGAACATCAGAACAGGACCCAGTGGCCAGTCTCTGGCTC CTCCCCCACCGCCTTACCGCCAGCCTCCTGGGGTCCCCAATGGACCCTCAAGCCCCACCAATGAGTCAGC CCCTGAGCTGCCACAGAGACACAATTCTTTGCATAGGAAAACGCCAGGGCCCGTCAGAGGCCTTGCACCT CCTCCACCCACCTCAGCCACCCCCTCCTTGTTGAGTAACAGGCCACCTCCCCCAGCCCGAGAGCCTCCTA GCAGGGGAGCAGCTCCCCCGCCCCCACCGCCCATGATCCGAAATGGTGCCAGGGATGCTCCCCCTCCTCC CCCACCTTACCGGATGCATGGGTCAGAGCCACCAAGCAGAGGAAAGCCCCCACCTCCACCCTCCAGGACA CCAGCTGGGCCGCCCCCTCCCCCACCACCCCCCTTGCGGAATGGCCATAGAGACTCCATCACCACTGTCC GCTCCTTCTTGGATGACTTTGAATCCAAGTACTCCTTCCATCCAGTGGAGGACTTCCCTGCTCCAGAAGA ATATAAACATCTTCAAAGAGTATACCCCAGCAAAACAAACCGAGCTGCCCGTGGAGCCCCACCTCTCCCA CCCATTCTCAGGTGAAGCTTGGCGTAGTCCTGCTCCTCAGGAAAAGGATGGACCTCCTCTTCTCAGATGG CCTCCCAACCCTAATCCCTGCATGAGAGCTCCTGCAGTGTTTCTCCAGTGCAGCCAACCCAGGCTCTGAG CATCCTCCAGCCCAGCTCCATCTATGCATCTCCTCTCTGGACTCGGTGATTGGACTCTTTCTATTGGCAG TAGGGTCTACTCCCATCTCCACCTTCTTCCAGCAAGCCCTGCTAGCCAGAGGAGGAGAAAGCTTCCACGC CTCCTGCTTTCTTCCAGGGAAAGGTGCCTTGTTGTGGTGAATGGACTCACATTGGGCAGGGCAGAAAGTG TAACCCCTGTCTCTGTCATTCACCGAGGGCGAAGTGTGGTGTGTGCATTATAGCCCATAGTTTAGGAGGT TGGTGTGGCCAGAACTTCTGAGGAGAGGTGTTCCATGATGCAGGGAGGGGGTTTGCAGAGAAGTGATCTG TTTTAAAGGTCGGGTTACAGAAAGGACAAGGACATGGGTCTTCTTCATTTTCAAGTGTTTTGGTTTTGTT CTCATCCCCTTCCCCCATCCCAAGAGATGAGCAGTGCATACACACTAAGTACTGATCCGCTAACTAAACG GTAAAGGGCACGAAGCAACGGAGGGTCATTTCAGAGCCAGTCAGCTCTTGTGCAGCTAAGGGCCAGGGCT GTCCGAGCTCTGGGACCTCCTGCCCCTTCCCCAGGGTGCTAGGCTGAACTGTGTCCCTTCCCGATCCCAC AAGAGAAGAGCCACTTTGTTGAGGGCATTGTCTATTCTCAAGTCACAAACCCTACAGACTTCCTATAAGA CATGGAAAGATAGGGCCTTAGCCACAGCCTCCTGATGGCTTCAGTTTTTTCTGATTAACCCTGAAGTGGC CAGCAGCCTGAGTCCTTCAGCCCTTGGGGAAAGCTCCAGGGAGACTTAAGAAGGGAACCGAAGCCCTGGC TTCATGGGAGTGTCTGGCTCTTCAGGGCCAGTAAGGTCTTGGCAGCCTGCTCATAGCGAGACTCTCACAC CCATCAGACGGCAGGCTCTTCCTGTTTTCTCTGCCACCCACCCATACCTTACTGAGACTGCAGGGTTTAA GGCCACAGCGTAGAAAAGGGAGCTTCAGGAGGAATACAGAGTCCTGTAAGCCAAGCACAAAGACATAGGG AGGCATGCCGCCCGCCATCTAGGAGGTGGGTCTTCCCTGGCGGCCAGCAATACATCGTTTCTCAGTCTTC CAAGGTGTAGGGAAACAGCACAGGAAGTGGGTGTTCCAACGGAGGCCGCTCTGTTGGCCAGGAAGTGTCT CTTCTCCACTAGCAGTGTGGGGCGCCTGAACTCCACACCCAGAGAAGTTGGTTTCTGACAGTCCTTGACC TGGGCACAAGCTGCTCAGATCTTACATCACGGTAGGGAGACCAGGCTGTTCTCTGAGTTGGGTAGGGGAA GCTAGTGAAAAACTTCCAGATACACCATGGGGTTAAATTATCGTAGGGGCTGTCCTTTTTTTTTTTATTA TTATTATTATTTTTATTTTTATTCTCCGCCCACCCCACTCCACTCCCACCCCCTAAGACAGGGTTTCTCT GTGCATTCCTGGCTGTCCTGGAACTTATTTTCTAGACCAGGCTGGCCTCAAACTCAGAGATCCCCTGTCT CTGCCTCCCAGGTGCTGGGACTAAAGGTGTGCGCCACCACGCTTGGCTCATAGGGACCTTTCTAATCTAG TGGACCATAGGCTAAGGATCTGTGTGTAATGCCAGGTCAGACCCAAATCTTCTAGGACTGGAGAAAGTGG ACCTAACTTAGTTCATTTATTTATTGAGTGTTGCCCATTGCCAGCTCTTGGACCACTTGGACTTTTGGGA CAGGCAGTGGTTAGATCTTACCGGACTTTTAATTGTTCACTATTTATCTTCTTCTCAAATCTGTTAGCAG TTACGCTCCCAGCATCCTAACTTGAACAAAGGGAAGAGAGGGAAAAAAAGCTGACACTTGGAGAGTTCCA GGGTAGATTCTAATGGTTGAACTGTGAAGTCTTCCCATTTGTTGTTGAAATGGGAGCTGATGCCTTTTGT ACAATGTTATCAAAGTGTGAAAATTTTCCCTTACTGAAGCATAGACCATCAGATATATGCGTGCGTATTT ATGTATGTGTATATATATATATATGTATATACACACACACATATAGATTTGTATACACACACATACATCT ACTCACAAAGCCAAACCCGGGCTTTTTGCTTGGGCTGATGGTACCTTCCTCGACTTGACCTGCCACCCTT TCTAATCCCCAGAATCAGACTGTGCCGCGTGGGTATTGATGATATATTTTCGTTGTCTTTGCCTGGTGGA ACCACCACCAAAAAAAGGCTTGAATTTAGATCTACATGAGTGAGTTTGCTCCTCCAGTTAACGGATTGTG TTGATGACACAACCCCTAACTTAAATGTCTTTGCCTTATTCATCATTTGGTCCTGCTGTTAATAACAAAT TTATTATCATGTATATTTGCTATTGAAGATGGGAGTGTGATCCTCAAAATTGTTGGTCTGTTTTATATAT TGTACACAGCTTGTAATGCAGGTTTAAGGTAGCCGGGCTGTGAAAACCCTGACACTGTTCCCCTTTAAAC TGTCCTTGATGTCCTCATGGGAATGTCGTCTCTTCAGTGGAAGATTGGGTGGTCTCATGTTGAGGCTATT ACTTAGTCCCTTTAATCCCCTTGTCCCCTCCCAAAGGGGAGAGACATTGCCCAGCTAAGCAGCCAGAAGC TCTGTCACAGCTTTCGTGGTATTTTTCCCTAGTGGGAAACGCTGGATGGCTGTTTCTTACTGCCCTGCCC TGTCAGGCGGTGGTTCTGATTTTCAAATGAGCTAGATGCTCCTCTTTCCCTTTCTCCAGTCACCAAACCT GGACCAAAGTACTTCATATTCCAGGTGTGGGTTCCTCTCTCCTGGGATTTGCTGCAGCTGCAGAGCCCTG CCCTTTTCGCAGCCTTGGCCACTTGTGTGAGCCACTAGGGCCATCCCAGCCTGATGGACGGGAGGCGGTC CTCACCAGGAATTCAATTCATTCCCTGCCCCCTTTCCTAGCCTAGGCAGAGAAACTAGTCACTGGCCATT GCCCTGGGGGAATCCCCTCACGCAGTGGCTTCCCATCTTAGAACCCGGACTTACCTCCAGGCGGAGGTGA GGGGTGGGGAGTACTGTAAATAGACTTGCTGCAGAGCAACTCAGGGTTGGGGTATGTTTGAGTTCTCCTG ATCACTTGAAAACTAATCTGTAGGCTGAATGCTTCTGGAGTGTGGGGGAAATGTGACTCCGGGGTCCTTT GTCCTCTGAGGCTCTGCGGGTGGAGGGTAGGACTTCAGAGGCCCTTTGATAGCACGGCTGCGCTGTCTGC CCTTCTGGAAACCCAACCTGCCATCAGCTGTAAATCAGATTCTTCACATTCCAGGTGTAAGCTTTCCCTT CCCTGTGACTCTCAGTCCCTAGCTACATGGTCAGGGGGACTCTGTTAAACCACCCAAATTTAGGGAATGA GAGGGGAGAGAGGAGGCCATTAGGAGTCTGCCTTCAAGACTCATTTCTTGAAAACAAAATGGAAAAAAAA AAAGATAACCACCTTCAATCACAGGCAAAAACTAAACCAAGAAAAAAAAAAAAAAAAGCAAACAAACCAA AAAACCCAACAGCAACCATGTAACTGAAGGAAGGGTCTGGTTCCGCTCCATCCCGTGTTCATTGTGCCTT TACTTGTGTTAGATCTCTGTGCTTTCTTCCTCCCCCTCTATGACGTCATTAATTAAAGCCTTCCTTGATA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6494304 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6494304 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17228 same embryo
	EMAGE:17230 same embryo
	EMAGE:17229 same embryo
	EMAGE:17231 same embryo
	EurExpress:euxassay_008874 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source