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EMAGE:17273

Asf1b ASF1 anti-silencing function 1 homolog B (S. cerevisiae) ( MGI:1914179)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17273 EMAGE:17273 EMAGE:17273 EMAGE:17273 EMAGE:17273
"Pseudo-wholemount" of euxassay_001590. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001590_01 euxassay_001590_02 euxassay_001590_03 euxassay_001590_04
EMAGE:17273 EMAGE:17273 EMAGE:17273 EMAGE:17273 EMAGE:17273
euxassay_001590_05 euxassay_001590_06 euxassay_001590_07 euxassay_001590_08 euxassay_001590_09
EMAGE:17273 EMAGE:17273 EMAGE:17273 EMAGE:17273 EMAGE:17273
euxassay_001590_10 euxassay_001590_11 euxassay_001590_12 euxassay_001590_13 euxassay_001590_14
EMAGE:17273 EMAGE:17273 EMAGE:17273 EMAGE:17273 EMAGE:17273
euxassay_001590_15 euxassay_001590_16 euxassay_001590_17 euxassay_001590_18 euxassay_001590_19
EMAGE:17273 EMAGE:17273 EMAGE:17273 EMAGE:17273
euxassay_001590_20 euxassay_001590_21 euxassay_001590_22 euxassay_001590_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17273Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17273_wholemount_strong.wlz
17273_wholemount_moderate.wlz
17273_wholemount_weak.wlz
17273_wholemount_possible.wlz
17273_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17273_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
thymus primordium
strong strong
homogeneousstrong expression: see section 08 moderate expression: see section 09 10 11 12
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 04 05 06 14 15 16 17
vibrissa
moderate moderate
regionalmoderate expression: see section 03 04 05 18 19
hypothalamus ventricular layer
moderate moderate
regionalmoderate expression: see section 11 12 13
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 11 12
olfactory cortex ventricular layer
strong strong
homogeneousstrong expression: see section 09 10 14 15 16
telencephalon ventricular layer
strong strong
homogeneousstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
rest of cerebellum marginal layer
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 13 14 15 16 17 18 19
rest of cerebellum ventricular layer
moderate moderate
regionalmoderate expression: see section 04 05 06 07 13 14 15 16 17 18 19
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 12 13 14
otic capsule
moderate moderate
regionalmoderate expression: see section 01 02 19 20 21 22
neural retina
moderate moderate
regionalmoderate expression: see section 01 02 21 22 23
liver lobe
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 22
exoccipital bone
moderate moderate
regionalmoderate expression: see section 22 23
temporal bone
moderate moderate
regionalmoderate expression: see section 23
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 03 04 17 19 20 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5690
Entity Detected:Asf1b, ASF1 anti-silencing function 1 homolog B (S. cerevisiae) ( MGI:1914179)
Sequence:sense strand is shown

>T5690
GCTGAGCGGAGGAGCGCTTCAGAGGGAAGAGTTGGGAGACGCAGGGAGGTTTCCGTCCTGTCGTCCCCCC
CTTCTTCTCTGTCGCAAGTCACTGTGAAGAAGTCTCCACAGCAGCTGCGGCTCCCGGGCGCGGTGATGGC
CAAGGTGTCGGTGCTGAATGTGGCTGTGCTGGAGAACCCGAGCCCTTTCCACAGCCCCTTCCGGTTCGAG
ATCAGCTTCGAATGCAGTGAGGCCCTGTCTGACGACCTGGAGTGGAAGATCATTTATGTGGGCTCAGCTG
AGAGTGAGGAGTTTGATCAGATCCTAGATTCAGTGCTGGTGGGCCCTGTCCCTGCAGGAAGGCATATGTT
CGTCTTTCAGGCTGATGCCCCAAACCCATCCCTCATTCCTGAGACGGACGCCGTGGGTGTGACTGTGGTT
CTCATCACCTGCACCTACCATGGACAAGAGTTCATCCGTGTGGGCTACTATGTCAACAATGAGTACCCAG
ACCCAGAGCTTCGAGAGAACCCACCCCCAAAGCCAGACTTCTCTCAGCTACAGCGGAACATCTTGGCCTC
TAACCCCCGGGTGACCCGATTCCATATCAACTGGGACAACAATCCAGACAGTCTGGAAGCCATAGAAAAC
CAGGACCCCAATGTGGACTTCAGCCTTTCCCTCAGTTGCACTCCTGTTAAAAGCCTGGGCCTCCCTAGTT
GCATCCCAGGCCTCCTCCCTGAGAACTCCATGGATTGCATCTGACAGGGGAGCCAGGTAGAGCTCAGTAG
GACCACAGCAGTCTCCTTGCACACCTGGAGGGGACCTACAAGCCCTCAGTCAGACATGCTTGGTCATCTC
AAGGATTTGGGGACCACCAGCTGTCTCCAGATCTGTCAGCCCCAAGAAAAAACAATGGGCCCCAATCTCT
TCCATCCAGGTCCAGAAGAATCATCTCACTTATGACCTACTGACCTCTAAAAAGCAGGTACTTGTATTGC
TCATTCTATGCGTCATTTTTCTTTATATATTATCAGTTTCTATGAGATGCTTTCTGAACACCACCTTCTG
TGAGGCTTGCTTGGGCAGTGTCTGGCTAGCATTGGCCAGAGGTTATCACTCCTTCCAACTCTAGTTCTTG
GGGACTAGGCAAGGAAGAAGTCCTAGTTCGAAGGCCAGCTCAATACAATGGCTATCCTTTCAGAGTGTCT
AGGACATTCAGTCTAACTAGTACTACAAAACGCAAAAGTGAGCTCCCCAGGCCAATGAGGGATGTCAGGT
ACTTTGGTCACAGCCACCTTTTCATCTCCTGACTGAGCCTGGCTTTCTGAGAGGATTGCCCTAGGTAGGT
ATATTGGAGGTCCTGCTTCATGCATTCTGGCCCCATAGAGTCATCAGGGTTTGCTTTGACCACATGTTCA
TTCTCATGCTATACCCAAATACTCTGGGATAAAGGAGGTGTTTGGCAGGCTGACCTGAAAAACTCCCATT
CCATCCACCTCTATCCTAAGCCCTTGGTTCCCTTGCTGCTACATGTATGCTATTGTGATTGCAGTGTGTA
TATTAAAAGGTTTTATATTAAAAGTTTTGGTTGTCTTAAGAATAAAAGCACTTCATCTAAAAAAAAAAAA
AAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:2645378 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2645378 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17272 same embryo
 EMAGE:17271 same embryo
 EMAGE:17276 same embryo
 EMAGE:17274 same embryo
 EMAGE:17275 same embryo
 EurExpress:euxassay_001590 same experiment
 MGI:4823275 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS